Biological activities of in vitro liverwort Marchantia polymorpha L. extracts
DOI:
https://doi.org/10.15835/nbha48211884Keywords:
biological activities; in vitro biomass; liverwort; Marchantia polymorpha L.Abstract
To overcome the problems in liverwort collecting such as small size and easily mixed with other species in the wild, we have successfully cultivated Marchantia polymorpha L. under in vitro conditions in the previous study. The aim of this study is to evaluate the biological activities of this in vitro biomass as a confirmation of the sufficient protocol in cultivation this species. Cultured biomass was dried at a temperature of 45-50 oC to constant weight and ground into a fine powder. The coarse powder was extracted with organic solvents of increasing polarization including n-hexane, chloroform, ethyl acetate, and ethanol using the maceration technique. Four extracts were investigated antioxidant (iron reduction power, DPPH), antibacterial (agar diffusion), tyrosinase inhibitory activity, anti-proliferation on MCF-7 cells. Additionally, the presence of natural metabolite groups of the extracts was detected by using specific reagents. For antioxidant activity, ethyl acetate fraction extract had the highest iron reducing power and DPPH free radical scavenging ability with IC50 = 439.31 µg ml-1. All three n-hexane, chloroform, and ethyl acetate extracts possessed resistance to the bacterial strain tested. At a concentration of 2 mg ml-1, n-hexane and chloroform extracts had the highest percentage of tyrosinase inhibition (69.54 and 69.10%, respectively). The n-hexane extract is a potent extract that inhibits the proliferation of MCF-7 cells with the lowest IC50 of 38.15 µg ml-1. A preliminary chemical composition survey showed that the cultured biomass liverwort contains many bioactive compounds, particularly the compounds of range of non- and less-polarized fractions.
Metrics
References
Abalaka ME, Mann A, Adeyemo SO (2011). Studies on in-vitro antioxidant and free radical scavenging potential and phytochemical screening of leaves of Ziziphus mauritiana L. and Ziziphus spinachristi L. compared with ascorbic acid. Journal of Medical Genetics and Genomics 3(2):28-34.
Amudha M, Rani S (2016). Evaluation of in vitro antioxidant potential of Cordia retusa. Indian Journal of Pharmaceutical Sciences 78(1):80-86.
Aneta S, Marina S, Jasmina G, Ana C, Milorad V, Boris P and Marko S (2011). Bio-activities of extracts from axenically farmed and naturally grow bryophytes. Journal of Medicinal Plants Research 5(4):565-571.
https://academicjournals.org/journal/JMPR/article-full-text-pdf/A4D3C9F26089
Asakawa Y (1995). Chemical constituents of the Bryophytes. In: Herz W, Kirby GW, Moore RE, Steglich W, Tamm CH (Eds.) Progress on the chemistry of organic natural products, Springer-Verlag, Vienna 65:1-618.
Asakawa Y, Ludwiczuk A (2013). Bryophytes: liverworts, mosses, and hornworts: extraction and isolation procedures. In: Roessner U, Dias D (Eds). Metabolomics Tools for Natural Product Discovery. Methods in Molecular Biology (Methods and Protocols). Humana Press, Totowa, NJ 1055:1-20. https://doi.org/10.1007/978-1-62703-577-4_1
Asakawa Y, Ludwiczuk A (2017). Chemical constituents of bryophytes: structures and biological activity. Journal of Natural Products 81:641-660. https://doi.org/10.1021/acs.jnatprod.6b01046
Asakawa Y, Ludwiczuk A, Nagashima F (2013). Phytochemical and biological studies of bryophyte. Phytochemistry 91:52-80. https://doi.org/10.1016/j.phytochem.2012.04.012
Asakawa Y, Tori M, Masuya T, Frahm JP (1990). Ent-sesquiterpenoids and cyclic bis(bibenzyls) from the German liverwort Marchantia polymorpha L. Phytochemistry 29(5):1577-1584.
Asakawa Y, Toyota M, Tori M, Hashimoto T (2000). Chemical structures of macrocyclic bis-(bibenzyls) isolated from liverworts (Hepaticae). Journal of Spectroscopy 14(4):149-175. https://doi.org/10.1155/2000/570265
Balouiri M, Sadiki M, Ibnsouda SK (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis 6(2):71-79. https://doi.org/10.1016/j.jpha.2015.11.005
Bowmann JL, Floyd SK, Sakakibara K (2007). Green genes-comparative genomics of the green branch of life. Cell 129:229-234. https://doi.org/10.1016/j.cell.2007.04.004
Chandra S, Chandra D, Barh A, Pandey R K, and Sharma IP (2016). Bryophytes: hoard of remedies, an ethno-medicinal review. Journal of Traditional and Complementary Medicine 7(1):94-98. https://doi.org/10.1016/j.jtcme.2016.01.007
Divya N, Vijaya Anand A (2015). In vitro antioxidant activity of ethanolic extract of Terminalia catappa leaves. Asian Journal of Pharmaceutical and Clinical Research 8(5):244-246.
Fischer S, Bottcher U, Reuber S, Anhalt S, Weissenbock G (1995). Chalcone synthase in the liverwort Marchantia polymorpha. Phytochemistry 39:1007. https://doi.org/10.1016/0031-9422(95)00097-Q
Gahtori D, Chaturvedi P (2011). Antifungal and antibacterial potential of methanol and chloroform extracts of Marchantia polymorpha L. Archives of Phytopathology and Plant Protection 44(8):726-731. https://doi.org/10.1080/03235408.2010.516083
Gawel-Beben K, Osika P, Asakawa Y, Antosiewicz B, Glowniak K, Ludwiczuk A (2019). Evaluation of anti-melanoma and tyrosinase inhibitory properties of marchantin A, natural macrocyclis bisbibenzyl isolated from Marchantia species. Phytochemistry Letters 31:192-195. https://doi.org/10.1016/j.phytol.2019.04.008
Ghasemi K, Ghasemi Y, Ebrahimzadeh MA (2009). Antioxidant activity, phenol and flavonoid contents of 13 citrus species peels and tissues. Pakistan Journal of Pharmaceutical Sciences 22(3):277-281. http://www.pjps.pk/wp-content/uploads/pdfs/CD-PJPS-22-3-09/Paper-7.pdf
Gokbulut A, Satilmis B, Batcioglu K, Cetin B, Sarer E (2012). Antioxidant activity and luteolin content of Marchantia polymorpha L. Turkish Journal of Biology 36:381-386. https://doi.org/10.3906/biy-1106-15
Hanh NTM, Phung NKP, Phuong QND (2017). Studying on tyrosinase inhibition activity of some Vietnamese folk plants aims to use in skin-whitening cosmetics. American Journal of Plant Sciences 8:1319-1328. https://www.scirp.org/pdf/AJPS_2017052315321467.pdf
Hans DZ, Hans B, Theophil E (1991). Bryophytes, a source of biologically active, naturally occurring material. Angewandte Chemie International Edition in English 30:130-147. https://doi.org/10.1002/anie.199101301
Harborne JB (1984). Phytochemical methods; A guide to modern techniques of plant analysis. London: Chapman and Hall (2nd ed) pp 4-16.
Huang WJ, Wu CL, Lin CW, Chi LL, Chen PY, Chiu CJ, … Chen CN (2010). Marchantin A, a cyclic bis (bibenzyl ether), isolated from the liverwort Marchantia emarginata subsp. tosana induces apoptosis in human MCF-7 breast cancer cells. Cancer Letters 291:108-119. https://doi.org/10.1016/j.canlet.2009.10.006
Jahan N, Parvin MS, Das N, Islam MS, Islam ME (2014). Studies on the antioxidant activity of ethanol extract and its fractions from Pterygota alata leaves. Journal of Acute Medicine 4(3):103-108. https://doi.org/10.1016/j.jacme.2014.05.001
Jayaveera KN, Yoganandham RK, Govindarajula Y, Kumanan R (2010) Phytochemical screenings, antibacterial activity and physical chemical constants of ethanolic extract of Euphorbia thymifolia. International Journal of Pharmacy and Pharmaceutical Sciences 2(3):81-82. https://innovareacademics.in/journal/ijpps/Vol2Issue3/579.pdf
Komala I, Ito T, Yagi Y, Nagashima F, Asakawa Y (2011). Cytotoxic bibenzyls, germacrane- and pinguisane- type sesquiterpenoids from the Indonesian, Tahitian and Japanese liverworts. Natural Product Communications 6(3):303-309.
Manmohan S, Arindam P, Hemendra PS (2014). Synthesis and reducing power assay of methyl semicarbazone derivates. Journal of Saudi Chemical Society 18(2):121-127. https://doi.org/10.1016/j.jscs.2011.06.004
Markham KR, Porter IJ (1974). Flavonoids of liverwort Marchantia polymorpha. Phytochemistry 13:1937-1942. https://doi.org/10.1016/0031-9422(74)85120-4.
My-Nuong TN, Thuy-Duong HH (2016). Selective cytotoxicity of a Vietnamese traditional formula, Nam Dia Long, against MCF-7 cells by synergistic effects. BMC Complementary and Alternative Medicine 16:220-230. https://doi.org/10.1186/s12906-016-1212-z
Nada C, Katarina S, Teodora J, Dejan P, Gordana Z, Svettana (2016). Optimization of polyphenols extraction from dried chokeberry using maceration as traditional technique. Food Chemistry 194:135-142. https://doi.org/10.1016/j.foodchem.2015.08.008
Remya K and Murugan K (2013). Polyphenols from Marchantia polymorpha L. a bryophyte: a potential source as antioxidant. World Journal of Pharmacy and Pharmaceutical Sciences 2(6):5182-5198. https://www.wjpps.com/Wjpps_controller/abstract_id/558
Sabovljevic MS, Vujicic M, Wang X, Garraffo HM, Bewley CA and Sabovljevic A (2017). Production of the macrocyclic bis-bibenzyls in axenically farmed and wild liverwort Marchantia polymorpha L. subsp. ruderalis Bisch. et Boisselier. Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology 151 (3):414-418. https://doi.org/10.1080/11263504.2016.1179692
Shimamura M (2015). Marchantia polymorpha: taxonomy, phylogeny and morphology of a model system. Plant and Cell Physiology 57(2):230-256. https://doi.org/10.1093/pcp/pcv192
Singh M, Singh S, Nath V, Sahu V, Singh RAK (2011). Antibacterial activity of some bryophytes used traditionally for the treatment of burn infections. Pharmaceutical Biology 49(5):526-530. https://doi.org/10.3109/13880209.2010.523007
Tan TQ, Hoang PN, Vy LN, Anh BL, Nhut DT, Phuong QND (2020). Improving in vitro biomass and evaluating α-glucosidase inhibition activity of liverwort Marchantia polymorpha L. Asian Journal of Plant Sciences 19:133-145. http://dx.doi.org/10.3923/ajps.2020.133.145
Tanaka M, Esaki T, Kenmoku H, Koeduka T, Kiyoyama T, Masujima T, … Matsui K (2016). Direct evidence of specific localization of sesquiterpenes and marchantin A in oil body cells of Marchantia polymorpha L. Phytochemistry 130:77-84. https://doi.org/10.1016/j.phytochem.2016.06.008.
Venkatesan T, Choi YW, Kim YK (2019). Impact of different extraction solvents on phenolic content and antioxidant potential of Pinus densiflora bark extract. BioMed Research International 2910 (ID 3520675). https://doi.org/10.1155/2019/3520675
Downloads
Published
How to Cite
Issue
Section
License
License:
Open Access Journal:
The journal allows the author(s) to retain publishing rights without restriction. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author.
.png)
