Effect of salinity stress on yield and quality parameters in flax (Linum usitatissimum L.)
DOI:
https://doi.org/10.15835/nbha48211861Keywords:
antioxidant enzymes; carotenoid; flax; flavonoids; phenolics; salt stressAbstract
Flax is one of the oldest utilitarian plants that have been grown for fibre as well as an oilseed crop. The species has been recognized as an excellent source of micronutrients, dietary fibre, protein, vitamin B1, lignan, and essential fatty acids (EFA), namely linoleic and α-linolenic acids. However, in spite of immense nutritional and industrial importance of the crop no work has been carried out on the effect of salinity induced by different salts on seed yield and quality traits in Linum usitatissimum. A high yielding germplasm line ‘Mukta’ was subjected to five concentrations (0 mM, 50 mM, 100 mM, 150 mM and 200 mM) of three salts viz. sodium chloride (NaCl), potassium chloride (KCl) and calcium chloride (CaCl2) for two seasons. Data were collected for seed yield along with different quality traits and a range of antioxidant enzymes. Seed yield decreased with rise in salt concentration and was minimum at 200 mM for all the salts. Chlorophyll a, chlorophyll b and carotenoid content continuously decreased with increase in salt concentration for all the salt treatments. Catalase and GST content increased with increase in salt concentration and was maximum at 200 mM.
References
Aebi H (1974). Catalase. In: Bergmeyer HU (Ed). Methods of enzymatic analysis, Verlag Chemie/Academic Press Inc, Weinheim/NewYork, pp 673-680.
Aghaei K, Ehsanpour AA, Komatsu S (2009). Potato responds to salt stress by increased activity of antioxidant enzymes. Journal of Integrative Plant Biology 51:1095-1103.
Akar Z, Küçük M, Doğan H (2017). A new colorimetric DPPH• scavenging activity method with no need for a spectrophotometer applied on synthetic and natural antioxidants and medicinal herbs. Journal of Enzyme Inhibition and Medicinal Chemistry 32:640-647.
Arora I, Sharma M, Tollefsbol TO (2019). Combinatorial Epigenetics Impact of Polyphenols and Phytochemicals in cancer prevention and therapy. International Journal of Molecular Sciences 20:E4567.
Bari G, Godward MBE (1970). Interspecific crosses in Linum. Euphytica 19:443-446.
Bayrak A, Kiralan M, Ipek A, Arslan N, Cosge B, Khawar KM (2010). Fatty acid composition of linseed (Linum usitatissimum L.) genotypes of different origin cultivated in Turkey. Biotechnology and Biotechnology Equipment 24:1836-1842.
Bennett MD, Leitch IJ (2004). Plant DNA C-values database (release 3.0).
Bhargava A, Shukla S, Katiyar RS, Ohri D (2003). Selection parameters for genetic improvement in Chenopodium grain in sodic soil. Journal of Applied Horticulture 5:45-48.
Bhattacharya A, Sood P, Citovsky V (2010). The roles of plant phenolics in defence and communication during Agrobacterium and Rhizobium infection. Molecular Plant Pathology 11:705-719.
Blois MS (1958). Antioxidant determinations by the use of a stable free radical. Nature 181:1199-1200.
Bose J, Rodrigo-Moreno A, Shabala S (2014). ROS homeostasis in halophytes in the context of salinity stress tolerance. Journal of Experimental Botany 65:1241-1257.
Boudet A (2007). Evolution and current status of research in phenolic compounds. Phytochemistry 68:2722-2735.
Bradford MM (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248-254.
Bui EN (2017). Causes of soil salinization, sodification, and alkalinization. Oxford Research Encyclopedia of Environmental Science.
Chaves MM, Flexas J, Pinheiro C (2009). Photosynthesis under drought and salt stress: Regulation mechanisms from whole plant to cell. Annals of Botany 103:551-560.
Chennaveeraiah MS, Joshi KK (1983). Karyotypes in cultivated and wild species of Linum. Cytologia 48:833-841.
Dai J, Mumper RJ (2010) Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules 15:7313-7352.
Das K, Roychoudhury A (2014). Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants. Frontiers in Environmental Science 2:53.
Demiral T, Turkan I (2005). Comparative lipid peroxidation, antioxidant defense systems and proline content in roots of two rice cultivars differing in salt tolerance. Environmental and Experimental Botany 53:247-257.
Durazzo A, Lucarini M, Souto EB, Cicala C, Caiazzo E, Izzo AA, Novellino E, Santini A (2019). Polyphenols: a concise overview on the chemistry, occurrence, and human health. Phytotherapy Research 33:2221-2243.
Esfandiari E, Gohari G (2017). Response of ROS-scavenging systems to salinity stress in two different wheat (Triticum aestivum L.) cultivars. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 45:287-291.
Ellman GL (1959). Tissue sulphydryl groups. Archives of Biochemistry and Biophysics 82:70-77.
Fatma A, Sokindra K, Shah AK (2013). Estimation of total phenolic content, in-vitro antioxidant and anti-inflammatory activity of flowers of Moringa oleifera. Asian Pacific Journal of Tropical Biomedicine 3:623-627.
Feller U, Vaseva II (2014). Extreme climatic events: impacts of drought and high temperature on physiological processes in agronomically important plants. Frontiers in Environmental Sciences 2:39.
Foyer CH, Noctor G (2005). Redox homeostasis and antioxidant signaling: a metabolic interface between stress perception and physiological responses. The Plant Cell 17:1866-1875.
Fraga CG, Croft KD, Kennedy DO, Tomás-Barberán FA (2019) The effects of polyphenols and other bioactives on human health. Food and Function 10:514‐528.
Goyal A, Sharma V, Sihag MK, Singh AK, Arora S, Sabikhi L (2016). Fortification of dahi (Indian yoghurt) with omega-3 fatty acids using microencapsulated flaxseed oil microcapsules. Journal of Food Science and Technology 53:2422-2433.
Habig WH, Pabst MJ, Jacoby WB (1974). Glutathione S-transferases: the first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry 249:7130-7139.
Hair JF, Anderson RE, Tatham RL, Black WC (1992). Multivariate data analysis. New York, Macmillan Publishing Company.
Harris BD (1968). Chromosome numbers and evolution in North American species of Linum. American Journal of Botany 55:1197-1204.
Heim KE, Tagliaferro AR, Bobilya DJ (2002). Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships. Journal of Nutritional Biochemistry 13:572-584.
Hernández JA (2019). Salinity Tolerance in Plants: Trends and Perspectives. International Journal of Molecular Sciences 20:2408.
Heywood VH (1993). Flowering plants of the world. Oxford, Oxford Univ. Press.
Hickey M (1988). 100 families of flowering plants. Cambridge Univerity Press, Cambridge, UK.
Hu Y, Fromm J, Schmidhalter U (2005). Effect of salinity on tissue architecture in expanding wheat leaves. Planta 220:838-848.
Infostat (2019). InfoStat, Versión 2019. Grupo InfoStat, Facultad de Ciencias Agrarias, Universidad Nacional de Córdoba, Córdoba, Argentina.
Jakovljević DZ, Topuzović MD, Stanković MS, Bojović BM (2017). Changes in antioxidant enzyme activity in response to salinity-induced oxidative stress during early growth of sweet basil. Horticulture, Environment and Biotechnology 58:240-246.
Jamil A, Riaz S, Ashraf M, Foolad MR (2011). Gene expression profiling of plants under salt stress. Critical Reviews in Plant Science 30:435-458.
Jensen A (1978). Chlorophylls and carotenoids. In: Hellebust JA, Craigie JS (Eds). Handbook of physiological methods: physiological and biochemical methods, Cambridge University Press, Cambridge, pp 5-70.
Jiang N, Doseff AI, Grotewold E (2016). Flavones: From biosynthesis to health benefits. Plants (Basel) 5:27.
Kajla P, Sharma A, Sood DR (2015). Flaxseed- a potential functional food source. Journal of Food Science and Technology 52:1857-1871.
Kakkar PS, Das B, Viswanathan PN (1984). A modified spectrophoto-metric assay of superoxide dismutase. Indian Journal of Biochemistry and Biophysics 21:130-132.
Kawai Y (2018). Understanding metabolic conversions and molecular actions of flavonoids in vivo: toward new strategies for effective utilization of natural polyphenols in human health. Journal of Medical Investigation 65:162-165.
Kedare SB, Singh RP (2011). Genesis and development of DPPH method of antioxidant assay. Journal of Food Science and Technology 48:412-422.
Kevin AL, Emmanuel G, Ellen P (2002). Flavonoid quantification in onion by spectrophotometric and high-performance liquid chromatography analysis. HortScience 37:682-685.
Khataar, M, Mohammadi M, Shabani F (2018). Soil salinity and matric potential interaction on water use, water use efficiency and yield response factor of bean and wheat. Scientific Reports 8:2679.
Kikuchi M (1929). Cytological studies of the genus Linum L. Japanese Journal of Genetics 4:202-212.
Ksouri R, Megdiche W, Debez A (2007). Salinity effects on polyphenol content and antioxidant activities in leaves of the halophyte Cakile maritime. Plant Physiology and Biochemistry 45:244-249.
Lalhminghlui K, Jagetia GC (2018). Evaluation of the free-radical scavenging and antioxidant activities of Chilauni, Schima wallichii Korth in vitro. Future Science OA 4:FSO272.
Mahdi-Pour B, Jothy SL, Latha LY, Chen Y, Sasidharan S (2012). Antioxidant activity of methanol extracts of different parts of Lantana camara. Asian Pacific Journal of Tropical Biomedicine 2:960-965.
Manach C, Scalbert A, Morand C, Remesy C, Jimenez L (2004). Polyphenols: food sources and bioavailability. American Journal of Clinical Nutrition 79:727-747.
McDill J, Repplinger M, Simpson BB, Kadereit JW (2009). The phylogeny of Linum and Linaceae subfamily Linoideae, with implications for their systematics, biogeography, and evolution of heterostyly. Systematic Botany 34:386-405.
Meng F, Luo Q, Wang Q, Zhang X, Qi Z, Xu F, Lei X, Cao Y, Chow WS, Sun G (2016). Physiological and proteomic responses to salt stress in chloroplasts of diploid and tetraploid black locust (Robinia pseudoacacia L.). Scientific Reports 6:23098.
Mensor LL, Menezes FS, Leitao GG, Reis AS, dos Santos TC, Coube CS, Leitao SG (2001). Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytotherapy Research 15:127-130.
Mhamdi A, Queval G, Chaouch S, Vanderauwera S, Van Breusegem F, Noctor G (2010). Catalase function in plants: a focus on Arabidopsis mutants as stress-mimic models. Journal of Experimental Botany 61:4197-4220.
Mohamed E, Matsuda R., El-Khatib AA, Takechi K, Takano H, Takio S (2016). Differential tolerance to high salt with regard to cell growth and superoxide dismutase (SOD) activity in calluses of the halophyte Suaeda maritima from Japan and Egypt. Plant Omics Journal 9:81-89.
Munns R, Tester M (2008). Mechanisms of salinity tolerance. Annual Review of Plant Biology 59:651-681.
Muravenko OV, Lemesh VA, Samatadze TE, Amosova AV, Grushetskaya ZE, Popov VK, … Zelenin AV (2003). Genome comparisons with chromosomal and molecular markers for three closely related flax species and their hybrids. Russian Journal of Genetics 39:414-421.
Nounjan N, Theerakulpisut P (2012). Effects of exogenous proline and trehalose on physiological responses in rice seedlings during salt-stress and after recovery. Plant Soil and Environment 58:309-315.
Oomah BD, Kenaschuk EO, Mazza G (1995). Phenolic acids in flaxseed. Journal of Agricultural and Food Chemistry 43:2016-2019.
Pandolfi C, Bazihizina N, Giordano C, Mancuso S, Azzarello E (2017). Salt acclimation process: a comparison between a sensitive and a tolerant Olea europaea cultivar. Tree Physiology 37:380-388.
Parvin K, Hasanuzzaman M, Bhuyan MHMB, Nahar K, Mohsin SM, Fujita M (2019). Comparative physiological and biochemical changes in tomato (Solanum lycopersicum L.) under salt stress and recovery: role of antioxidant defense and glyoxalase systems. Antioxidants (Basel) 8:350.
Rahman MM, Rahman MA, Miah MG, Saha SR, Karim MA and Mostofa MG (2017). Mechanistic insight into salt tolerance of Acacia auriculiformis: the importance of ion selectivity, osmoprotection, tissue tolerance, and Na+ exclusion. Frontiers in Plant Science 8:155.
Rahneshan Z, Nasibi F, Moghadam AA (2018). Effects of salinity stress on some growth, physiological, biochemical parameters and nutrients in two pistachio (Pistacia vera L.) rootstocks, Journal of Plant Interactions 13:73-82.
Robertson KR (1971). The Linaceae in the southeastern United States. Journal of Arnold Arboretum 52: 649-665.
Rogers CM, Harris BD (1966). Some new chromosome numbers in Linum. Madrono 18:179-180.
Russo R, Reggiani R (2015). Phenolics and antioxidant activity in flax varieties with different productive attitude. International Food Research Journal 22:1736-1739.
Sairam RK, Tyagi A (2004). Physiology and molecular biology of salinity stress tolerance in plants. Current Science 86:407-421.
Scandalios JG (2002). The rise of ROS. Trends in Biochemical Sciences 27:483-486.
Shah SH, Houborg R, McCabe MF (2017). Response of chlorophyll, carotenoid and spad-502 measurement to salinity and nutrient stress in wheat (Triticum aestivum l.). Agronomy 7:61.
Shahbaz M, Ashraf M (2013). Improving salinity tolerance in cereals. Critical Reviews in Plant Sciences 32:237-249.
Shalata A, Mittova V, Volokita M, Guy M, Tal M (2001). Response of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii to salt-dependent oxidative stress: the root antioxidative system. Physiologia Plantarum 112:487-494.
Shrivastava P, Kumar R (2015). Soil salinity: A serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. Saudi Journal of Biological Science 22:123-131.
Singh KK, Mridula D, Rehal J, Barnwal P (2011). Flaxseed: a potential source of food, feed and fiber. Critical Reviews in Food Science and Nutrition 51:210-222.
Sowndhararajan K, Kang SC (2013). Free radical scavenging activity from different extracts of leaves of Bauhinia vahlii Wight & Arn. Saudi Journal of Biological Sciences 20:319-325.
Strogonov BP, Kabanov VV, Lapina LP, Prykhodko LS (1970). Structure and function of plant cells under salinity conditions. Nauka Publishing House Moscow.
Szymańska R, Ślesak I, Orzechowska A, Kruk J (2017) Physiological and biochemical responses to high light and temperature stress in plants. Environmental and Experimental Botany 139:165-177.
Taïbi K, Taïbi F, Abderrahim LA, Ennajah A, Belkhodja M, Mulet JM (2016). Effect of salt stress on growth, chlorophyll content, lipid peroxidation and antioxidant defence systems in Phaseolus vulgaris L. South African Journal of Botany 105:306-312.
Tang L, Ying RR, Jiang D, Zeng XW, Morel JL, Tang YT, Qiu RL (2013). Impaired leaf CO2 diffusion mediates cd-induced inhibition of photosynthesis in the zn/cd hyperaccumulator Picris divaricata. Plant Physiology and Biochemistry 73:70-76.
Tardieu F (2013). Plant response to environmental conditions: assessing potential production, water demand, and negative effects of water deficit. Frontiers in Physiology 4:17.
Tohge T, Watanabe M, Hoefgen R, Fernie, AR (2013). The evolution of phenylpropanoid metabolism in the green lineage. Critical Reviews in Biochemistry and molecular Biology 48:3123-152.
Tutin TG, Heywood VH, Burges NA, Murre DM, Valentine DH, Walters SM, Webb DM (1968). Flora Europaea: Rosaceae to Umbelliferae. Cambridge, Cambridge Univ. Press.
Valderrama R, Corpas FJ, Carreras A, Gomez-Rodriguez MV, Chaki M, Pedrajas JR, … Barroso JB (2006). The dehydrogenase-mediated recycling of NADPH is a key antioxidant system against salt-induced oxidative stress in olive plants. Plant Cell and Environment 29:1449-1459.
Vromans J (2006). Molecular genetic studies in flax (Linum usitatissimum L.). Ph.D. diss. Wageningen, Wageningen University.
Weisany W, Sohrabi Y, Heidari G, Siosemardeh A, Ghassemi-Golezani K (2012). Changes in antioxidant enzymes activity and plant performance by salinity stress and zinc application in soybean (Glycine max L.). Plant Omics Journal 5:60-67.
Yang T, Poovaiah BW (2002). Hydrogen peroxide homeostasis: Activation of plant catalase by calcium/calmodulin. PNAS 99:4097-4102.
Yonekura-Sakakibara K, Higashi Y, Nakabayashi R (2019). The origin and evolution of plant flavonoid metabolism. Frontiers in Plant Science 10:943.
Zeven AC (1982). Dictionary of cultivated plants and their centers of diversity, excluding ornamentals, forest trees and lower plants. Center for Agricultural Publishing and Documentation, Wageningen, The Netherlands.
Zohary D, Hopf M (2000). Domestication of plants in the old world. Oxford, Oxford University Press.
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