Optimization cultivation of Chlamydomonas reinhardtii in a tubular photobioreactor (2000 Liter) for biomass and green bioenergy (biodiesel) production
Keywords:biodiesel production; C. reinhardtii; lipids; photobioreactor
The biodiesel can be produced from diverse microalgae lipids as alternative and renewable fuel. Thus, the aim of this study was to optimize the Chlamydomonas reinhardtii promising species as biodiesel feedstock for large-scale cultivation in Egypt. To understand some of the triggers required for the metabolic pathway switch to lipid accumulation, the effect of carbon sources and the three elements availability (N, P, S) in C. reinhardtii growth medium was determined. A local microalgae C. reinhardtii was cultured in modified Sueoka medium containing various concentrations of CO2 and bicarbonate (NaHCO3) (in 2-liter flasks) as a carbon source. The optimal source in term biomass, high lipid productivity (10.3 mgL-1d-1) and a higher lipid content (22.76%) were obtained in 6% CO2 culture. Then, the availability of N, P, S (various concentrations of N, P and S) nutrients elements was added to 6% CO2 culture, for produce a highest lipid content and lipid productivity. As expected, under low availability N-1.78 mM; P-0.14mM and S-0.10 mM mediums, C. reinhardtii showed a high accumulation lipid content. Therefore, to improve the economic feasibility of microalgae biofuels production, its concentrations were selected to combine (N+P+S) in order to cultivation of C. reinhardtii in a multi-tubular photobioreactor (400 liter) to produce high lipid contents. Under limited condition, the biomass dry weight, biomass productivity, lipid content and lipid productivity were found to be 3.11 (gL-1), 0.15±0.012 (g-1L-1d-1), 22.76% (w/w %) and 1.9± 0.35 (mg-1L-1d-1), respectively. The extracted lipid was found to have physical and chemical properties similar that plant oils using for biodiesel production. The FAME profiling of prepared biodiesel shows the presence of considerable amount of 36.97% saturated fatty acids (palmitic acid and stearic acid, together) with 27.33% unsaturated (oleic acid and linoleic acid) fatty acids. The FAME had a low iodine value and high CN, which meet with the appropriate of biodiesel standards (EN 14214 and ASTM D6751). Thus, C. reinhardtii appears to be more feasible for high quality biodiesel production.
Abd El Baky HH, El Baroty GS, Bouaid A, Mercedes M, Aracil J (2012). Enhancement of lipid accumulation in Scenedesmus obliquus by optimizing CO2 and Fe3+ levels for biodiesel production. Bioresource Technology 119: 429-432. https://doi.org/10.1016/j.biortech.2012.05.104
Abd El Baky HH, El-Baroty GS (2013). The potential use of microalgal carotenoids as dietary supplements and natural preservative ingredient. Journal of Aquatic Food Product Technology 4:392-406. https://doi.org/10.1080/10498850.2011.654381
Abd El Baky HH, El-Baroty GS (2016). Potential of macroalgae Ulva lactuca L as a source feedstock for biodiesel production. Recent Patents on Food, Nutrition & Agriculture 8(3):199-204. https://doi.org/10.2174/2212798409666170602080725
Abd El Baky HH, El-Baroty GS, Bouaid A (2014). Lipid induction in Dunaliella salina culture aerated with various levels CO2 and its biodiesel production. Journal of Aquaculture Research and Development 5:1-6. https://doi.org/10.4172/2155-9546.1000223
Abd El Baky HH, El-Baroty GS, Ibrahem AE (2015). Functional characters evaluation of biscuits sublimated with pure phycocyanin isolated from Spirulina and Spirulina biomass. Nutricion Hospitalaria 32(1):231-241
American Oil Chemists’ Society (2004). Official Methods and Recommended Practices of the AOCS, 5th ed.; AOCS Press: Boulder, CO, USA.
Benvenuti G, Bosma R, Cuaresma M, Janssen M, Barbosa, MJ, Wijffels RH (2015). Selecting microalgae with high lipid productivity and photosynthetic activity under nitrogen starvation. Journal of Applied Phycology 27(4):1425-1431. https://doi.org/10.1007/s10811-014-0470-8
Cesar AD, Conejero MA, Ribeiro EC, Batalha MA (2019). Competitiveness analysis of “social soybeans” in biodiesel production in Brazil. Renewable Energy 133:1147-1157.
Davey MP, Alison G, Smith AG, Horst I, Duong G, Tomsett BV, … Howe CG (2014). Triacylglyceride production and autophagous responses in Chlamydomonas reinhardtii depend on resource allocation and carbon source. Eukaryotic Cell 13 (3):392-400. https://doi.org/10.1128/EC.00178-13
Demirbas A (2009). Progress and recent trends in biodiesel fuels. Energy Conversion and Management 50:14-34. https://doi.org/10.1016/j.enconman.2008.09.001
Demirbas A (2011). Biodiesel from oilgae, biofixation of carbon dioxide by microalgae: a solution to pollution problems. Applied Energy 88:3541-3547. https://doi.org/10.1016/j.apenergy.2010.12.050
El-Kassas HY (2013). Growth and fatty acid proﬁle of the marine microalga Picochlorum Sp. grown under nutrient stress conditions. Egyptian Journal of Aquatic Research 39:233-239. http://dx.doi.org/10.1016/j.ejar.2013.12.007
Fan LH, Zhang YT, Zhang L, Chen HL (2008). Evaluation of a membrane-sparged helical tubular photobioreactor for carbon dioxide bioﬁxation by Chlorella vulgaris. Journal of Membrane Science 325:336-345. https://doi.org/10.1016/j.memsci.2008.07.044
Giakoumis EG (2013). A statistical investigation of biodiesel physical and chemical properties, and their correlation with the degree of unsaturation. Renewable Energy 50:858-878. https://doi.org/10.1016/j.renene.2012.07.040
Goh BH, Ong HC, Cheah MY, Chen W, Yu KL Mahlia TM (2019) Sustainability of direct biodiesel synthesis from microalgae biomass: A critical review. Renewable and Sustainable Energy Reviews 107:59-74. https://doi.org/10.1016/j.rser.2019.02.012
Griffiths MJ, Harrison ST (2009). Lipid productivity as a key characteristic for choosing algal species for biodiesel production. Journal of Applied Phycology 21:493-507. https://doi.org/10.1007/s10811-008-9392-7.
Hoekman SK, Broch A, Robbins C, Ceniceros E, Natarajan M (2012). Review of biodiesel composition, properties, and speciﬁcations. Renewable and Sustainable Energy Reviews 16:143-169. http://dx.doi.org/10.1016/j.rser.2011.07.143
Hu Q, Sommerfeld M, Jarvis E, Ghirardi M, Posewitz M, Seibert M, Darzins A (2008). Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant Journal 54:621-639. https://doi.org/10.1111/j.1365-313X.2008.03492.x
James GO, Hocart CH, Hillier W, Chen H, Kordbacheh F, Price D, Djordjevic MA (2011). Fatty acid proﬁling of Chlamydomonas reinhardtii under nitrogen deprivation. Bioresource Technology 102:3343-3351. https://doi.org/10.1016/j.biortech.2010.11.051
Juergens MT, Disbrow B, Shachar-Hill Y (2016). The relationship of triacylglycerol and starch accumulation to carbon and energy ﬂows during nutrient deprivation in Chlamydomonas reinhardtii, Plant Physiology 171:2445-2457. https://doi.org/10.1104/pp.16.00761
Kaisan MU, Pam GY, Kulla DM, Kehinde AJ (2015). Effects of oil extraction method on biodiesel production from wild grape seeds: a case study of Soxhlet extraction method and mechanical press engine driven expeller method. STM - Journal of Alternate Energy and Technologies 6(1):35-41.
Karpagam R, Preeti R, Ashokkumar B, Varalakshmi P (2015). Enhancement of lipid production and fatty acid proﬁling in Chlamydomonas reinhardtii, CC1010 for biodiesel production. Ecotoxicology and Environmental Safety 121:253-257. https://doi.org/10.1016/j.ecoenv.2015.03.015
Kropat J, Hong-Hermesdorf A, Casero D, Ent P, Castruita M, Pellegrini M, Merchant SS, Malasarn D (2011) A revised mineral supplement increase biomass and growth rate in Chlamydomonas reinhardtii. Plant Journal 66:770-780. https://doi.org/10.1111/j.1365-313X.2011.04537.x
Lam MK, Lee KT (2014). Cultivation of Chlorella vulgaris in a pilot-scale sequential bafﬂed column photobioreactor for biomass and biodiesel production. Energy Conversion and Management 88:399-410.
Lee S, Kim TY (2017). Performance and emission characteristics of a DI diesel engine operated with diesel/DEE blended fuel. Applied Thermal Engineering 121:454-461. https://doi.org/10.1016/j.applthermaleng.,04.112
Lichtenthaler HK, Wellburn AR (1983). Determinations of total carotenoids and chlorophyll a and b in leaf extracts in different solvents. Biochemical Society Transactions 11:591-592. https://doi.org/10.1042/bst0110591
Ma Y, Gao Z, Wang Q, Liu Y (2018). Biodiesels from microbial oils: Opportunity and challenges. Bioresource Technology 263:631-641. https://doi.org/10.1016/j.biortech.2018.05.028
Martins AA, Marques F, Cameira M, Santos E, Sara B, Costa L, … Mata TM (2018). Water footprint of microalgae cultivation in photobioreactor. Energy Procedia 153:426-443. https://doi.org/10.1016/j.egypro.2018.10.031
Mathimani T, Mallick N (2018). A comprehensive review on harvesting of microalgae for biodiesel - Key challenges and future directions. Renewable and Sustainable Energy Reviews 91:1103-1120. https://doi.org/10.1016/j.rser.2018.04.083
Moser B (2009). Biodiesel production, properties, and feedstocks. In Vitro Cellular and Developmental Biology 45:229-266.
Moser BR (2014). Impact of fatty ester composition on low temperature properties of biodiesel-petroleum diesel blends. Fuel 115:500-506.
Nascimento IA, Marques SSI, Cabanelas ITD, Pereira SA, Druzian JI, De Souza CO, … Nascimento MA (2013). Screening microalgae strains for biodiesel production: Lipid productivity and estimation of fuel quality based on fatty acid profiles as selective criteria. Bioenergy Research 6:1-13.
Patel A, Arora N, Mehtani J, Pruthi V, Pruthi PA (2017). Assessment of fuel properties on the basis of fatty acid proﬁles of oleaginous yeast for potential biodiesel production. Renewable and Sustainable Energy Reviews 77 604-616. https://doi.org/10.1016/j.rser.2017.04.016
Ramos MJ, Fernández CM, Casas A, Rodríguez L, Pérez A (2009). Inﬂuence of fatty acid composition of raw materials on biodiesel properties. Bioresource Technology 100(1):261-268. http://dx.doi.org/10.1016/j.biortech.2008.06.039
Sajjadi B, Chen W, Raman AA, Ibrahim S (2018). Microalgae lipid and biomass for biofuel production: A comprehensive review on lipid enhancement strategies and their eﬀects on fatty acid composition. Renewable and Sustainable Energy Reviews 97:200-232. https://doi.org/10.1016/j.rser.2018.07.050
Singh P, Guldhe A, Kumari S, Rawat I, Bux F (2015). Investigation of combined eﬀect of nitrogen, phosphorus and iron on lipid productivity of microalgae Ankistrodesmus falcatus KJ671624 using response surface methodology. Biochemical Engineering Journal 94:22-29. https://doi.org/10.1016/j.bej.2014.10.019
Singh TS, Verma TN, Nashine P, Shijagurumayum C (2018). BS-III diesel vehicles in imphal, India: an emission perspective. In: Sharma N, Agarwal A, Eastwood P, Gupta T, Singh A (Eds). Air Pollution and Control. Energy, Environment, and Sustainability. Springer, Singapore, 978-981-10-7184-3. https://doi.org/10.1007/978-981-10-7185-0_5
Sueoka N, Chiang, KS, Kates JR (1967). Deoxyribonucleic acid replication in meiosis of Chlamydomonas reinhardtii. Isotopic transfer experiments with a strain producing eight zoospores. Journal of Molecular Biology 25:44-67. https://doi.org/10.1016/0022-2836(67)90278-1
Sun LY, Cui WJ, Chen KM (2018). Two Mychonastes isolated from freshwater bodies are novel potential feedstocks for biodiesel production. Energy sources, Part A: Recovery, utilization, and environmental effects 40(12):1452-1460. https://doi.org/10.1080/15567036.2018.1477869
Tang D, Han W, Li P, Miao X, Zhong J (2009). CO2 bioﬁxation and fatty acid composition of Scenedesmus obliquus and Chlorella pyrenoidosa in response to different CO2 levels. Bioresource Technology 102(3):3071-3076. https://doi.org/10.1016/j.biortech.2010.10.047
Wang Y, Yu J, Wang P, Deng S, Chang J, Ran Z (2018). Response of energy microalgae Chlamydomonas reinhardtiito to nitrogen and phosphors stress. Environmental Science and Pollution Research 25(6):5762-5770. https://doi.org/10.1007/s11356-017-0931-0
Wang ZT, Ullrich, N, Joo, S, Waffenschmidt S, Goodenough U. (2009) Algal lipid bodies. Stress induction, purification, and biochemical characterization in wild-type and starchless Chlamydomonas reinhardtii. Eukaryotic Cell 8:1856-1868. https://doi.org/10.1128/EC.00272-09
Wu H, Miao X (2014). Biodiesel quality and biochemical changes of microalgae Chlorella pyrenoidosa and Scenedesmus obliquus in response to nitrate levels. Bioresource Technology 170:421-427. https://doi.org/10.1016/j.biortech.2014.08.017
Zhang L, Wang N, Yang M, Ding K, Yong-Zhong Wang Y, Huo D, Hou C (2019). Lipid accumulation and biodiesel quality of Chlorella pyrenoidosa under oxidative stress induced by nutrient regimes. Renewable Energy 143:1782-1790. https://doi.org/10.1016/j.renene.2019.05.081
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