Effect of PEG-induced drought stress on germination of ten chickpea (Cicer arietinum L.) genotypes

  • Avraam KOSKOSIDIS University of Thessaly, School of Agricultural Science, Laboratory of Genetics and Plant Breeding, Fytokou Str., 38446 Volos
  • Ebrahim KHAH University of Thessaly, School of Agricultural Science, Laboratory of Genetics and Plant Breeding, Fytokou Str., 38446 Volos
  • Athanasios MAVROMATIS Aristotle University of Thessaloniki, Laboratory of Genetics and Plant Breeding, School of Agriculture, 54124 Thessaloniki
  • Ourania PAVLI University of Thessaly, School of Agricultural Science, Laboratory of Genetics and Plant Breeding, Fytokou Str., 38446 Volos
  • Dimitrios N. VLACHOSTERGIOS Hellenic Agricultural Organization Demeter, Institute of Industrial and Forage Crops, 41335 Larissa
Keywords: chickpea; drought; germination; tolerance; water stress

Abstract

Chickpea (Cicer arietinum L.) is one of the most important legume crops worldwide, with its importance mainly relying on its high protein content. Chickpea productivity is strictly threatened by abiotic stresses, of which drought exerts the most crucial role in terms of growth inhibition and yield losses encountered. Given that germination is a critical stage that is negatively affected by drought, the aim of this study was to estimate the genotypic variability among ten chickpea genotypes and to determine the seed germination and seedling growth ability under drought stress conditions. Seeds were subjected to water stress by polyethylene glycol (PEG-6000) at five stress levels (0, 5, 10, 20, 30 and 50% PEG). Germination percentage, seed water content, seed water absorbance, root and shoot development and seedling vigour index were evaluated. The analyses revealed significant genetic variability in relation to genotypic performance under drought stress. Drought significantly affected germination as well as all other associated traits, with the effects of stress being analogous to the stress level applied. Findings point to the fact that seedling vigour index is a suitable selection criterion for drought tolerance as it allowed the classification of genotypes as tolerant, moderately tolerant, moderately susceptible and susceptible. Taken together, the commercial varieties ‘Thiva’, ‘Keryneia’ and ‘Gavdos’ as well as the landrace ‘Lemnos’ showed an increased drought tolerance at high stress level, indicating their possible exploitation as valuable genetic material for breeding programs or for commercial use.

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Published
2020-03-31
How to Cite
KOSKOSIDIS, A., KHAH, E., MAVROMATIS, A., PAVLI, O., & VLACHOSTERGIOS, D. N. (2020). Effect of PEG-induced drought stress on germination of ten chickpea (Cicer arietinum L.) genotypes. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48(1), 294-304. https://doi.org/10.15835/nbha48111799
Section
Research Articles