Evaluation of the cytogenetic effects of Quantis biostimulant in Allium sativum cells

  • Aurel L. OLARU University of Craiova, Faculty of Agronomy, 19 Libertatii Street, Craiova
  • Elena ROSCULETE University of Craiova, Faculty of Agronomy, 19 Libertatii Street, Craiova
  • Elena BONCIU University of Craiova, Faculty of Agronomy, 19 Libertatii Street, Craiova
  • Catalin A. ROSCULETE University of Craiova, Faculty of Agronomy, 19 Libertatii Street, Craiova
  • Ioan SARAC Banat’s University of Agricultural Science and Veterinary Medicine „King Michael I of Romania”, 119 Calea Aradului Street, Timisoara
Keywords: cytotoxicity; garlic; genotoxicity; mitotic index

Abstract

The integration of plant biostimulants use to improve the efficiency of crop production can contribute to increasing the agronomic efficiency. Through this study we aimed to investigate the cytogenetic effects of the Quantis biostimulant on the meristematic cells of Allium sativum (garlic), at different concentrations (1, 1.5 and 2.5%) for 8 and 24 h. The results obtained showed that, at 1 and 1.5% concentration, Quantis induced the increase of the mitotic division intensity in A. sativum cells, both during at 8 and 24 h of treatment, compared to the untreated control variant. This fact suggests that, at the respective concentrations, Quantis improves the growth and development processes of the plant. In contrast, at 2.5% concentration, the index of mitotic division decreased by 36.6% (at 8 h), respectively by 64.9% (at 24 h of treatment), compared to the control. Also, at 2.5% Quantis, some chromosomal aberrations and nuclear anomalies were recorded: sticky and laggards’ chromosomes, C-mitosis, micronucleus, nuclear bud and nuclear dissolution. These results suggest that when is used in high concentrations, Quantis induces some cytotoxic and genotoxic effects on A. sativum, which can disrupt the plant growth.

Metrics

Metrics Loading ...

References

Abu Ngozi E, Ezeugwu SC (2008). Risk evaluation of industrial wastewater on plants using onion (Allium cepa L.) chromosome aberration assay. Journal of Tropical Agriculture, Food, Environment and Extension 7(3):242-248.

Adegbite AE, Ayodele MS, Odunbaku KR, Idehen EO (2009). Mutagenic effect of Neem leaf extract used in traditional medicine on Allium cepa (L.). Scientific Research and Essays 4(11):1315-1321.

Akaneme FI, Iyioke IV (2008). Mutagenic potentials of the sterilizing fluid-Purital on root tip mitosis of Allium cepa. Bio-Research 6(1):293-297.

Ali RT, Abdel-Ghany EM, Mohamed FI, Hanna EM, Elashery ZM (2019). Usage of the cyto-genetics and cytology to identify the action mechanisms of two biofertilizers on Allium cepa meristematic cells. Bulletin of the National Research Centre 43:138.

Asli S, Neumann PM (2010). Rhizosphere humic acid interacts with root cell walls to reduce hydraulic conductivity and plant development. Plant and Soil 336:313-322.

Bonciu E, Roșculete E, Olaru AL, Roșculete CA (2018). Evaluation of the mitodepresive effect, chromosomal aberrations and nuclear abnormalities induced by urea fertilization in the meristematic tissues of Allium cepa L. Caryologia 71:350-356.

Bostan C, Butnariu M, Butu M, Ortan A, Butu A, Rodino S, Parvu C (2013). Allelopathic effect of Festuca rubra on perennial grasses. Romanian Biotechnological Letters 18(2):8190-8196.

Bulgari R, Franzoni G, Ferrante A (2019). Biostimulants application in horticultural crops under abiotic stress conditions. Agronomy 9:306.

Butnariu M (2012). An analysis of Sorghum halepense's behavior in presence of tropane alkaloids from Datura stramonium extracts. Chemistry Central Journal 6:75.

Butnariu M, Caunii A (2013). Design management of functional foods for quality of life improvement. Annals of Agricultural and Environmental Medicine 20(4):736-741.

Cerdán M, Sánchez-Sánchez A, Oliver M, Juárez M, Sánchez-Andreu JJ (2009). Effect of foliar and root applications of aminoacids on iron uptake by tomato plants. Acta Horticulturae 830:481-488.

Cerdan M, Sanchez-Sanchez A, Jorda JD, Juarez M, Sanchez-Andreu J (2013). Effect of commercial amino acids on iron nutrition of tomato plants grown under lime-induced iron deficiency. Journal of Plant Nutrition and Soil Science 176:859-866.

Chen J, Lü S, Zhang Z, Zhao X, Li X, Ning P, Liu M (2018). Environmentally friendly fertilizers: A review of materials used and their effects on the environment. Science of the Total Environment 613:829-839.

Csizinszky AA (1990). Response of two bell peppers (Capsicum annuum L.) cultivars to foliar and soil-applied biostimulants. Soil and Crop Science Society of Florida Proceedings 49:199-203.

du Jardin P (2015). Plant biostimulants: definition, concept, main categories and regulation. Scientia Horticulturae 196:3-14.

Firbas P, Amon T (2014). Chromosome damage studies in the onion plant Allium cepa L. Caryologia 67(1):25-35.

Gajc-Wolska J, Radzanowska J, Lyszkowska M (2009). The influence of grafting and biostimulators on physical and sensorial traits of greenhouse tomato fruit (Lycopersicon esculentum Mill.) in field production. Acta Scientiarum Polonorum Hortorum Cultus 8:37-43.

Georgieva N, Nikolova I, Dimitrov V, Dimitrova D (2016). Economic evaluation of forage pea organic production. Banat's Journal of Biotechnology VII(14):60-67.

Heckman JR (1995). Evaluating phosphorus fertilization and commercial biostimulants for producing cabbage. HortTechnology 5:298-300.

Hossain MM, Jahan I (2015). Azospirillum as biofertilizer and Bangladesh perspective. Banat's Journal of Biotechnology VI(11):69-82.

Janas KM, Posmyk MM (2013). Melatonin, an underestimated natural substance with great potential for agricultural application. Acta Physiologiae Plantarum 35:3285-3292.

Kaushik PS, Ramya M, Gargi GP (2017). Mitodepressive activity of two common food dyes on Allium cepa L. root meristem. International Journal for Research in Applied Science & Engineering Technology 5(IX):954-967.

Kara M, Sanda MA, Ates A (1994). Cytogenetic effects of the insecticide Cypermethin on the root meristems of Allium cepa L. Turkish Journal of Biology 18:323-331.

Kelting M, Harris JR, Fanelli J, Appleton B, Niemiera A (1997). Humate-based biostimulants do not consistently increased growth of container-grown turkish hazelnut. Journal of Environmental Horticulture 15:197-199.

Khanna N, Sharma S (2013). Allium cepa root chromosomal aberration assay: a review. Indian Journal of Pharmaceutical and Biological Research 1(3):105-119.

Kirn A, Kashif SR, Yaseen M (2010). Using indigenous humic acid from lignite to increase growth and yield of okra (Abelmoschus esculentus L.). Soil & Environment 29:187-191.

Leme DM, Marin-Morales MA (2009). Allium cepa test in environmental monitoring: A review on its application. Mutation Research 682(1):71-81.

Liman R, Ciğerci IH, Öztürk NS (2015). Determination of genotoxic effects of Imazethapyr herbicide in Allium cepa root cells by mitotic activity, chromosome aberration, and comet assay. Pesticide Biochemistry and Physiology 118:38-42.

Lisiecka J, Knaflewski M, Spizewski T, Fraszczak B, Kaluzewicz A, Krzesinski W (2011). The effect of animal protein hydrolysate on quantity and quality of strawberry daughter plants cv ‘Elsanta’. Acta Scientiarum Polonorum Hortorum Cultus 10:31-40.

Liu E, Yan C, Mei X, He W, Bing SH, Ding L (…) Fan T (2010). Long-term effect of chemical fertilizer, straw, and manure on soil chemical and biological properties in northwest China. Geoderma 158(3):173-180.

Lyszkowska M, Gajc-Wolska J, Kubi K (2008). The influence of biostimulators on yield and quality of leaf and iceberg lettuce - grown under field conditions. In: Dbrowski ZT (Ed). Biostimulators in modern agriculture. Wie Jutra Press, Warszawa pp 28-34.

Neri D, Lodolini EM, Savini G, Sabbatini P, Bonanomi G, Zucconi F (2002). Foliar application of humic acids on strawberry (cv. Onda). Acta Horticulturae 594:297-302.

Osuna D, Prieto P, Aguilar M (2015). Control of seed germination and plant development by carbon and nitrogen availability. Frontiers in Plant Science 6:1023.

Özkara A, Akyıl D, Eren Y, Erdoğmuş SF (2015). Potential cytotoxic effect of Anilofos by using Allium cepa assay. Cytotechnology 67(5):783-791.

Pandia O, Sărăcin I, Chiriac A, Bozga I, Oancea M, Ticu C (2012). Determination of NPK in some local populations of pepper in order to obtain adequate food compliant with the EU food safety rules. Scientific Papers. Series A. Agronomy 55:369-372.

Pandia O, Sărăcin I, Sărăcin AI (2018). Management of agricultural culture establishment works. Scientific Papers Series Management, Economic Engineering in Agriculture and Rural Development 18(2):315-318.

Prokkola S, Kivijärvi Parikka P (2003). Effects of biological sprays, mulching materials, and irrigation methods on grey mould in organic strawberry production. Acta Horticulturae 626:169-175.

Ricci M, Tilbury L, Daridon B, Sukalac K (2019). General principles to justify plant biostimulant claims. Frontiers in Plant Science 10:494.

Rosculete CA, Bonciu E, Rosculete E, Olaru LA (2019). Determination of the environmental pollution potential of some herbicides by the assessment of cytotoxic and genotoxic effects on Allium cepa. International Journal of Environmental Research and Public Health 16:75.

Ruiz JM, Castilla N, Romero L (2000). Nitrogen metabolism in pepper plants applied with different bioregulators. Journal of Agricultural and Food Chemistry 48:2925-2929.

Samfira I, Butnariu M, Rodino S, Butu M (2013). Structural investigation of mistletoe plants from various hosts exhibiting diverse lignin phenotypes. Digest Journal of Nanomaterials and Biostructures 8(4):1679-1686.

Saporta R, Bou C, Frías V, Mulet JM (2019). A method for a fast evaluation of the biostimulant potential of different natural extracts for promoting growth or tolerance against abiotic stress. Agronomy 9:143.

Sharma S, Vig AP (2012). Genotoxicity of atrazine, avenoxan, diuron and quizalofop-P-ethyl herbicides using the Allium cepa root chromosomal aberration assay. Terrestrial and Aquatic Environmental Toxicology 6(2):90-95.

Szparaga A, Kubo M, Kocira S, Czerwinska E, Pawłowska A, Hara P (…) Kwasniewski D (2019). Towards sustainable agriculture-agronomic and economic effects of biostimulant use in common bean cultivation. Sustainability 11:4575.

Tarantino A, Lops F, Disciglio G, Lopriore G (2018). Effects of plant biostimulants on fruit set, growth, yield and fruit quality attributes of ‘Orange Rubis®’ apricot (Prunus armeniaca L.) cultivar in two consecutive years. Scientia Horticulturae 239:26-34.

Valiente AC, Salgado YR, Remigio Montero AC, Arnaez GP, Fernandez Esperon N, Mancebo Rodriguez A (…) Ocana Arzuagain R (2013). In vivo genotoxic evaluation of biological and organic pesticides and fertilizers. Science International 1:98-102.

Vasileva V (2015). Root biomass accumulation in vetch (Vicia sativa L.) after treatment with organic fertilizer. Banat's Journal of Biotechnology VI(11):100-105.

Yüzbaşioğlu D, Ünal F, Sancak C, Kasap R (2003). Cytological effects of the herbicide racer “flurochloridone” on Allium cepa. Caryologia 56(1):97-105.

Zhang L, Yan C, Guo Q, Zhang J, Ruiz-Menjivar J (2018). The impact of agricultural chemical inputs on environment: global evidence from informetrics analysis and visualization. International Journal of Low-Carbon Technologies 13(4):338-352.

Published
2020-06-30
How to Cite
OLARU, A. L., ROSCULETE, E., BONCIU, E., ROSCULETE, C. A., & SARAC, I. (2020). Evaluation of the cytogenetic effects of Quantis biostimulant in Allium sativum cells. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48(2), 681-691. https://doi.org/10.15835/nbha48211788
Section
Research Articles