Identification and characterization of a circadian clock-associated pseudo-response regulator 7 gene from trifoliate orange

Authors

  • Yu-E DING Yangtze University, College of Horticulture and Gardening, Jingzhou, Hubei 434025 (CN)
  • Wenkai HUANG Huazhong Agricultural University, College of Life Science and Technology, Wuhan 430070 (CN)
  • Bo SHU Yangtze University, College of Horticulture and Gardening, Jingzhou, Hubei 434025 (CN)
  • Ying-Ning ZOU Yangtze University, College of Horticulture and Gardening, Jingzhou, Hubei 434025 (CN) http://orcid.org/0000-0003-2607-2689
  • Qiang-Sheng WU Yangtze University, College of Horticulture and Gardening, Jingzhou, Hubei 434025; University of Hradec Kralove, Faculty of Science, Department of Chemistry, Hradec Kralove 50003 (CN)
  • Kamil KUČA University of Hradec Kralove, Faculty of Science, Department of Chemistry, Hradec Kralove 50003 (CZ) http://orcid.org/0000-0001-9664-1109

DOI:

https://doi.org/10.15835/nbha48111785

Keywords:

citrus; diurnal rhythm; drought; pseudo-response regulator

Abstract

Circadian clock is usually involved in many physiological processes of plants, including responses to abiotic stress, whilst pseudo-response regulator 7 (PRR7) gene is the main component of the circadian clock. In this study, the cDNA of the PRR7 gene was obtained from trifoliate orange (Poncirus trifoliata). Based on the sequence analysis, the PtPRR7 gene had an open reading frame of 2343 bp, encoded 780 amino acids, and contained proteins of the REC and CCT domains. Subcellular localization indicated that PtPRR7 was mainly localized in the nucleus and a small amount of cytoplasm. qRT-PCR analysis revealed the highest expression level of PtPRR7 in roots than in both shoots and leaves. The PtPRR7 gene during 24 hours of soil water deficit exhibited a circadian rhythmic expression pattern: the expression peak at 9:00 am in leaves and at 21:00 pm in roots. Drought treatment affected PtPRR7 gene expression. Such data provide important references for understanding the characteristics of PtPRR7 gene in citrus plants.

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Published

2020-03-31

How to Cite

DING, Y.-E., HUANG, W., SHU, B., ZOU, Y.-N., WU, Q.-S., & KUČA, K. (2020). Identification and characterization of a circadian clock-associated pseudo-response regulator 7 gene from trifoliate orange. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48(1), 128–139. https://doi.org/10.15835/nbha48111785

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Research Articles
CITATION
DOI: 10.15835/nbha48111785