Identification and characterization of a circadian clock-associated pseudo-response regulator 7 gene from trifoliate orange

  • Yu-E DING Yangtze University, College of Horticulture and Gardening, Jingzhou, Hubei 434025
  • Wenkai HUANG Huazhong Agricultural University, College of Life Science and Technology, Wuhan 430070
  • Bo SHU Yangtze University, College of Horticulture and Gardening, Jingzhou, Hubei 434025
  • Ying-Ning ZOU Yangtze University, College of Horticulture and Gardening, Jingzhou, Hubei 434025 http://orcid.org/0000-0003-2607-2689
  • Qiang-Sheng WU Yangtze University, College of Horticulture and Gardening, Jingzhou, Hubei 434025; University of Hradec Kralove, Faculty of Science, Department of Chemistry, Hradec Kralove 50003
  • Kamil KUČA University of Hradec Kralove, Faculty of Science, Department of Chemistry, Hradec Kralove 50003 http://orcid.org/0000-0001-9664-1109
Keywords: citrus; diurnal rhythm; drought; pseudo-response regulator

Abstract

Circadian clock is usually involved in many physiological processes of plants, including responses to abiotic stress, whilst pseudo-response regulator 7 (PRR7) gene is the main component of the circadian clock. In this study, the cDNA of the PRR7 gene was obtained from trifoliate orange (Poncirus trifoliata). Based on the sequence analysis, the PtPRR7 gene had an open reading frame of 2343 bp, encoded 780 amino acids, and contained proteins of the REC and CCT domains. Subcellular localization indicated that PtPRR7 was mainly localized in the nucleus and a small amount of cytoplasm. qRT-PCR analysis revealed the highest expression level of PtPRR7 in roots than in both shoots and leaves. The PtPRR7 gene during 24 hours of soil water deficit exhibited a circadian rhythmic expression pattern: the expression peak at 9:00 am in leaves and at 21:00 pm in roots. Drought treatment affected PtPRR7 gene expression. Such data provide important references for understanding the characteristics of PtPRR7 gene in citrus plants.

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References

Atamian HS, Harmer SL (2016). Circadian regulation of hormone signaling and plant physiology. Plant Molecular Biology 91:691-702.

Bhattacharya A, Khanale V, Char B (2017). Plant circadian rhythm in stress signaling. Indian Journal of Plant Physiology 22:147-155.

Chen H, Zhang J, Neff MM, Hong SW, Zhang H, Deng XW, Xiong L (2008). Integration of light and abscisic acid signaling during seed germination and early seedling development. Proceedings of the National Academy of Sciences 105:4495-4500.

Covington MF, Maloof JN, Straume M, Kay SA, Harmer SL (2008). Global transcriptome analysis reveals circadian regulation of key pathways in plant growth and development. Genome Biology 9:R130.

De Caluwé J, Xiao Q, Hermans C, Verbruggen N, Leloup JC, Gonze D (2016). A compact model for the complex plant circadian clock. Frontiers in Plant Science 7:74.

Dixon DC, Cutt JR, Klessig DF (1991). Differetial targeting of the tobacco PR-1 pathogenesis-related proteins to the extracellular space and vacuoles of crystal idioblasts. The EMBO Journal 10:1317-1324.

Du SY, Zhang XF, Lu Z, Xin Q, Wu Z, Jiang T, Lu Y, Wang XF, Zhang DP (2012). Roles of the different components of magnesium chelatase in abscisic acid signal transduction. Plant Molecular Biology 80:519-537.

Elvira MI, Galdeano MM, Gilardi P, Garcialuque I, Serra M (2008). Proteomic analysis of pathogenesis-related proteins (PRs) induced by compatible and incompatible interactions of pepper mild mottle virus (PMMoV) in Capsicum chinense L3 plants. Journal of Experimental Botany 59:1253-1265.

Fang LJ, Qin RL, Liu Z, Liu CR, Gai YP, Ji XL (2019). Expression and functional analysis of a PR-1 gene, MuPR1, involved in disease resistance response in mulberry (Morus multicaulis). Journal of Plant Interactions 14:376-385.

Farré EM, Harmer SL, Harmon FG, Yanovsky MJ, Kay SA (2005). Overlapping and distinct roles of PRR7 and PRR9 in the Arabidopsis circadian clock. Current Biology 15:47-54.

Farré EM, Kay SA (2007). PRR7 protein levels are regulated by light and the circadian clock in Arabidopsis. The Plant Journal 52:548-560.

Farré EM, Liu T (2013). The PRR family of transcriptional regulators reflects the complexity and evolution of plant circadian clocks. Current Opinion in Plant Biology 16:621-629.

Filichkin SA, Breton G, Priest HD, Dharmawardhana P, Jaiswal P, Fox SE, … Mockler TC (2011). Global profiling of rice and poplar transcriptomes highlights key conserved circadian-controlled pathways and cis-regulatory modules. PLoS ONE 6:e16907.

Fukushima A, Kusano M, Nakamichi N, Kobayashi M, Hayashi N, Sakakibara H, Mizuno T, Saito K (2009). Impact of clock-associated Arabidopsis pseudo-response regulators in metabolic coordination. Proceedings of the National Academy of Sciences 106:7251-7256.

Garg A, Orru R, Ye W, Distler U, Chojnacki JE, Kohn M, … Wolf E (2019). Structural and mechanistic insights into the interaction of the circadian transcription factor BMAL1 with the KIX domain of the CREB-binding protein. Journal of Biological Chemistry 294(45):16604-16619.

Greenham K, Mcclung CR (2015). Integrating circadian dynamics with physiological processes in plants. Nature Reviews Genetics 16:598-610.

He JD, Dong T, Wu HH, Zou YN, Wu QS, Kuča K (2019). Mycorrhizas induce diverse responses of root TIP aquaporin gene expression to drought stress in trifoliate orange. Scientia Horticulturae 243:64-69.

He JD, Zou YN, Wu QS, Kuča K (2020). Mycorrhizas enhance drought tolerance of trifoliate orange by enhancing activities and gene expression of antioxidant enzymes. Scientia Horticulturae 262:108745.

Jain N, Vergish S, Khurana JP (2018). Validation of house-keeping genes for normalization of gene expression data during diurnal/circadian studies in rice by RT-qPCR. Scientific Reports 8:3203.

Juliana MG, Aparecida RF, Renata FP, Claire B, Thiago JN, Brandon C, … Alexandre N (2014). Diurnal oscillations of soybean circadian clock and drought responsive genes. PLoS ONE 9:e86402.

Khan S, Rowe SC, Harmon FG (2010). Coordination of the maize transcriptome by a conserved circadian clock. BMC Plant Biology 10:126.

Kim JA, Yang TJ, Kim JS, Park JY, Kwon SJ, Lim MH, Jin M, Lee SC, Lee SI, Choi BS, Um SH, Kim HI, Chun C, Park BS (2007). Isolation of circadian-associated genes in Brassica rapa by comparative genomics with Arabidopsis thaliana. Molecules and Cells 23:145-153.

Li J, Liu YH, Zhang Y, Chen C, Yu X, Yu SW (2017). Drought stress modulates diurnal oscillations of circadian clock and drought-responsive genes in Oryza sativa L. Hereditas 39:837-846.

Liu T, Carlsson J, Takeuchi T, Newton L, Farre EM (2013). Direct regulation of abiotic responses by the Arabidopsis circadian clock component PRR7. The Plant Journal 76:101-114.

Published
2020-03-31
How to Cite
DING, Y.-E., HUANG, W., SHU, B., ZOU, Y.-N., WU, Q.-S., & KUČA, K. (2020). Identification and characterization of a circadian clock-associated pseudo-response regulator 7 gene from trifoliate orange. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48(1), 128-139. https://doi.org/10.15835/nbha48111785
Section
Research Articles