Chitosan nanoparticle and pyridoxine seed priming improves tolerance to salinity in milk thistle seedling

  • Ali Asghar MOSAVIKIA Islamic Azad University, Department of Agronomy, Birjand Branch, Birjand
  • Seyed Gholamreza MOSAVI Islamic Azad University, Department of Agronomy, Birjand Branch, Birjand
  • Mohammadjavad SEGHATOLESLAMI Islamic Azad University, Department of Agronomy, Birjand Branch, Birjand
  • Reza BARADARAN Islamic Azad University, Department of Agronomy, Birjand Branch, Birjand
Keywords: free proline content; photosynthetic pigments; salinity threshold; seed vigour index; sodium chloride

Abstract

Application of growth regulators plays important role under salt conditions. Perspectives to overcome these limitations by chitosan nanoparticle (CSNP: 0, 0.25, 0.5, and 1%) and pyridoxine (PN: 0, 0.03, 0.06, and 0.09%) seed priming was studied in both experiments with milk thistle seeds exposed to NaCl as salt stress (0, 50, 100, and 150 mM). Salinity threshold and EC50 (the salinity level that 50% of germination reduction) achieved 74.85 and 213.5 mM, respectively. A significant reduction in germination percentage (49.12%), seedling length (50.07%), and seedling vigor index (67.39%) while, a significant increase in superoxide dismutase activity (54.63%) were achieved at 150 mM NaCl in compared to the control treatment. The highest germination rate was resulted by 100 mM NaCl and 0.25% CSNP and the least (2.86 seed/day) by 150 mM NaCl and without CSNP. The salt stress significantly decreased photosynthetic pigments; however, the largest value of chlorophyll a, b, and total was related to without NaCl and 1% CSNP and the least value of traits (6.1, 1.67, and 7.77 µg/g FW) to non-application of CSNP under 150 mM NaCl. PN application was caused decrease in free proline content compared to the non-application treatment. The most pronounced effects of CSNP and PN were recorded in 0.25 and 0.09% concentrations, respectively. The finding of this study leads to the conclusion that seed priming with CSNP and PN by improving physiological mechanisms such as photosynthetic pigment synthesis, antioxidant enzyme activities, and free proline content increased salt tolerance in milk thistle seedling.

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References

Aghighi Shahverdi M, Omidi H, Tabatabaei SJ (2017). Effect of nutri-priming on germination indices and physiological characteristics of stevia seedling under salinity stress. Journal of Seed Science 39(4):353-362.

Aghighi Shahverdi M, Omidi H, Tabatabaei SJ (2019). Stevia (Stevia rebaudiana Bertoni) responses to NaCl stress: Growth, photosynthetic pigments, diterpene glycosides and ion content in root and shoot. Journal of the Saudi Society of Agricultural Sciences 18:355-360.

Andrzejewska J, Sadowska K, Mielcarek S (2011). Effect of sowing date and rate on the yield and flavolignan content of the fruits of milk thistle (Silybum marianum L. Gaertn.) on light soil in a moderate climate. Industrial Crops and Products 33:462-468.

Anusuya S, Banu KN (2016). Silver-chitosan nanoparticles induced biochemical variations of chickpea (Cicer arietinum L.). Biocatalysis and Agricultural Biotechnology 8:39-44.

Bates L, Waldren R, Teare I (1973). Rapid determination of free proline for water-stress studies. Plant and Soil 39:205-207.

Beauchamp C, Fridovich I (1971). Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry 44:276-287.

Burguieres E, McCue P, Kwon YI, Shetty K (2007). Effect of vitamin C and folic acid on seed vigor response and phenolic-linked antioxidant activity. Bioresource Technology 98:1393-1404.

Cha-Um S, Kirdmanee C (2009). Effect of salt stress on proline accumulation, photosynthetic ability and growth characters in two maize cultivars. Pakistan Journal of Botany 41:87-98.

Demidchik V (2015). Mechanisms of oxidative stress in plants: from classical chemistry to cell biology. Environmental and Experimental Botany 109:212-228.

Dolatabadian A, Modares Sanavy SAM, Chashmi NA (2008). The effects of foliar application of ascorbic acid (Vitamin C) on antioxidant enzymes activities, lipid peroxidation and proline accumulation of canola (Brassica napus L.) under conditions of salt stress. Journal of Agronomy and Crop Science 194:206-213.

El-Keblawy A, Al-Rawai A (2005). Effects of seed maturation time and dry storage on light and temperature requirements during germination in invasive Prosopis juliflora. Flora 201:135-143.

El-Miniawy SM, Ragab ME, Youssef SM, Metwally AA (2013). Response of strawberry plants to foliar spraying of chitosan. Research Journal of Agriculture and Biological Science 9(6):366-372.

Farooq M, Hussain M, Wakeel A, Siddique KHM (2015). Salt stress in maize: Effects, resistance mechanisms, and management. A review. Agronomy for Sustainable Development 35:461-481.

Farooq M, Wahid A, Kobayashi N, Fujita D, Basra SMA (2009). Plant drought stress: Effects, mechanisms, and management. In: Lichtfouse E, Navarrete M, Debaeke P, Véronique S, Alberola C (Eds). Sustainable Agriculture. Springer, Dordrecht, pp 153-188.

Ferreira T, Pavia I, Baltazar M, Rocha L, Pereira JM, Lima-Brito J, Correia C (2016). Vitamin B6 ameliorates germination and early growth of Triticum durum L. under water stress conditions. 24th International Symposium of the International Scientific Centre of Fertilizers. Coimbra (Portugal), September 6-8, 2016.

Genuchten MTh van (1983). Analysing crop salt tolerance data: model description and user’s manual. Research Report No. 120. U.S. Salinity Laboratory, USDA-ARS, Riverside, California.

Ghavami N, Ramin AA (2007). Salinity and temperature effects on seed germination of Milk thistle. Communications in Soil Science and Plant Analysis 38:2681-2691.

Ghoulam C, Fares K (2001). Effect of salinity on seed germination and seedling growth of sugar beet (Beta vulgaris L.). Seed Science and Technology 29:357-364.

Gornik K, Grzeisk B, Duda R (2008). The effect of chitosan on rooting of grapevine cuttings and on subsequent plant growth under drought and temperature stress. Journal of Fruit Ornamental Plant Research 16:333-343.

Gorzi A, Omidi H, Bostani AB (2018). Morpho-physiological responses of stevia (Stevia rebaudiana Bertoni) to various priming treatments under drought stress. Applied Ecology and Environmental Research 16(4):4753-4771.

Guan YJ, Hu J, Wang XJ, Shao CX (2009). Seed priming with chitosan improves maize germination and seedling growth in relation to physiological changes under low-temperature stress. Journal of Zhejiang University Science B 10(6):427-433.

Hameed A, Sheikh MA, Hameed A, Farooq T, Basra SMA, Jamil A (2014). Chitosan seed priming improves seed germination and seedling growth in Wheat (Triticum aestivum L.) under osmotic stress induced by polyethylene glycol. Philippine Agricultural Scientist 97(3):294-299.

Harish P, Dharmesh MS, Jagannatha Rao KS, Tharanatha RN (2007). Free radical-induced chitosan depolymerized products protect calf thymus DNA from oxidative damage. Carbohydrate Research 342(2):190-195.

Imran M, Bolet B, Muhling KH (2018). Zinc seed priming improves salt resistance in maize. Journal of Agronomy and Crop Science 1-10.

ISTA (2009). Workshop reports – ISTA online – International seed testing Association.

Kar M, Mishra D (1976). Catalase, peroxidase and polyphenol oxidase activity during rice leaf senescence. Plant Physiology 57:315-319.

Karkanis A, Bilalis D, Efthmiadou A (2011). Cultivation of milk thistle (Silybum marianum L. Gaertn.), a medicinal weed. Industrial Crops and Products 34:825-830.

Keshavarz H, Moghadam RSG (2017). Seed priming with cobalamin (vitamin B12) provides significant protection against salinity stress in the common bean. Rhizosphere 3:143-149.

Lauchli A, Luttge U (2012). Salinity: environment plants molecules. the Netherlands: Springer.

Lehmann S, Funck D, Szabados L, Rentsch D (2010). Proline metabolism and transport in plant development. Amino Acids 39(4):949-962.

Lichtenthaler HK (1987). Chlorophylls and carotenoids. Pigments of photosynthetic membranes. Method Enzyme 148:350-382.

Lipiec J, Doussan C, Nosalewicz A, Kondracka K (2013). Effect of drought and heat stresses on plant growth and yield: a review. International Agrophysics 27(4):463-477.

Misra N, Dwivedi UN (2004). Genotype differences in salinity tolerance of green gram cultivars. Plant Science 166:1135-1142.

Nasiri Y, Feyzi P, Javanmard A (2014). Effects of hydro and hormonal seed priming on seed germination of milk thistle under saline stress condition. Notulae Scientia Biologicae 6(3):374-380.

Parmoon G, Ebadi A, Jahanbakhsh S, Moosav SA (2015). Effects of seed priming on catalase activity and storage reservoirs of aged milk thistle seeds (Silybum marianum (L.) Gaertn). Journal of Agriculture Sciences 21:363-372.

Pongprayoon W, Roytrakul S, Pichayangkura R, Chadchawan S (2013). The role of hydrogen peroxide in chitosan-induced resistance to osmotic stress in rice (Oryza sativa L.). Plant Growth Regulation 70(2):159-173.

Qu C, Liu C, Gong X, Li C, Hong M, Wang L, Hong F (2012). Impairment of maize seedling photosynthesis caused by a combination of potassium deficiency and salt stress. Environmental and Experimental Botany 75:134-141.

Raschke M, Boycheva S, Crevecoeur M, Nunes-Nesi A, Witt S, Fernie AR, … Fitzpatrick TB (2011). Enhanced levels of vitamin B6 increase aerial organ size and positively affect stress tolerance in Arabidopsis. The Plant Journal 66:414-432.

Rosinska A, Dorna H, Szopinska D, Irzykowska L, Seidler-Lozykowska K (2018). Evaluation of milk thistle (Silybum marianum (L.) Gaertn.) seed germination in relation to seed health and seedling emergence. Herba Polonica 64(3):1-10.

Sadeghi H, Khazaei F, Yari L, Sheidaei S (2011). Effect of seed osmo-priming on seed germination behavior and vigor of soybean (Glycine max L.). ARPN Journal of Agriculture and Biological Science 6(1):39-43.

Sedghi M, Nemati A, Amanpour-Balanji B, Gholipouri A (2010). Influence of different priming materials on germination and seedling establishment of milk thistle (Silybum marianum) under salinity stress. World Applied Sciences Journal 11(5):604-609.

Sharma P, Jha AB, Dubey RS, Pessarakli M (2012). Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of Botany 12:1-26.

Sheikha SAAK, AL-Malki FM (2011). Growth and chlorophyll responses of bean plants to the chitosan application. European Journal of Scientific Research 50(1):124-134.

Suchada B, Sarobol E, Meechoui S, Sooksathan I (2007). Drought recovery and grain yield potential of rice after chitosan application. Kasetsart Journal (Natural Science) 41:1-6.

Sudhakar C, Lakshmi A, Giridara KS (2001). Changes in the antioxidant enzyme efficacy in two high yielding genotypes of mulberry (Mours alba L.) under NaCl salinity. Plant Science 167:613-619.

Sultana S, Islam M, Khatun MA, Hassain MA, Huque R (2017). Effect of foliar application of oligo-chitosan on growth, yield and quality of tomato and eggplant. Asian Journal of Agricultural Research 11(2):36-42.

Teresa BF (2011). Vitamins B6 in plants: more than meets the eye. Advances in Botanical Research 59:1-38.

Van Genuchtan MT, Hoffman GJ (1984). Analysis of crop salt tolerance data. Soil salinity under irrigation-process and management. Ecological Studies 51:258-271.

Verma S, Mishra SN (2005). Putrescine alleviation of growth in salt-stressed Brassica juncea by inducing antioxidative defense system. Journal of Plant Physiology 162:669-677.

Walker-Simmons M, Ryan CA (1984). Proteinase inhibitor synthesis in tomato leaves: induction by chitosan oligomers and chemically modified chitosan and chitin. Plant Physiology 76(3):787-790.

Yin H, Bai XF, Du YG (2008). The primary study of oligochitosan inducing resistance to Sclerotinia sclerotiorum on B. napus. Journal of Biotechnology 136:600-601.

Yin H, Xavier CF, Chrestensen LP, Grevsen K (2011). Chitosan oligosaccharides promote the content of polyphenols in Greek oregano (Oregamum vulgare ssp. Hirtum). Journal of Agricultural and Food Chemistry 60(1):136-143.

Published
2020-03-31
How to Cite
MOSAVIKIA, A. A., MOSAVI, S. G., SEGHATOLESLAMI, M., & BARADARAN, R. (2020). Chitosan nanoparticle and pyridoxine seed priming improves tolerance to salinity in milk thistle seedling. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48(1), 221-233. https://doi.org/10.15835/nbha48111777
Section
Research Articles