Phytochemical Composition and Antioxidant Activity of Various Grain Amaranth Cultivars


  • Andreea STĂNILĂ University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Faculty of Food Science and Technology, 3-5 Mănăștur Street 400372, Cluj-Napoca (RO)
  • Bogdan CIOANCA University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Faculty of Horticulture, 3-5 Mănăștur Street, 400372, Cluj-Napoca (RO)
  • Zorița DIACONEASA University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Faculty of Food Science and Technology, 3-5 Mănăștur Street 400372, Cluj-Napoca (RO)
  • Sorin STĂNILĂ University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Faculty of Agriculture, 3-5 Mănăștur Street, 400372, Cluj-Napoca (RO)
  • Nicușor SIMA University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Faculty of Animal Husbandry and Biotechnology, 3-5 Mănăștur Street, 400372, Cluj-Napoca (RO)
  • Rodica Maria SIMA University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Faculty of Horticulture, 3-5 Mănăștur Street, 400372, Cluj-Napoca (RO)



Amaranthus; antioxidant activity; flavonoids; manure; polyphenols; protein


This study quantified differences in methanolic extracts composition among four grain amaranth cultivars (e.g. ‘Hopy Red Dye’, ‘Amont’, ‘Plenitude’, and ‘Golden Giant’) farmed under three planting conditions: no irrigation/no fertilization (NN), no irrigation/fertilization (NF), irrigation/no fertilization (IN). The study main outcomes were total flavonoids, polyphenols, antioxidant activity, and protein content. Antioxidant activity was assessed using two Single Electron Transfer (SET) based assays: the 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) radical cation decolorization assay (ABTS) and cupric reducing antioxidant capacity (CUPRAC). The total protein content was assessed by Gornall spectrophotometric method, the total flavonoid content (TFC) was determined using a colorimetric technique, while total polyphenols content (TPC) was assessed using the Folin-Ciocalteu method. Mean differences in outcomes were calculated using ANOVA and Dunnet’s test for multiple comparisons. The findings revealed that TPC ranged from 5 to 18 mg gallic acid equivalents (GAE)/100 g dry weight (DW), being highest in ‘Plenitude’ under NF conditions. The highest TFC (7.5 mg quercetin equivalent (QE)/100 g DW) and the highest protein content (37.25%) were revealed for the ‘Hopi Red Dye’ cultivar under the NF planting conditions. Amaranth seeds represent a potential rich source of polyphenols and protein gluten-free compounds, with the ‘Hopi Red Dye’ representing the richest cultivar in such compounds. Fertilized and non-irrigated soil provided the optimal planting conditions across all amaranth cultivars.


Adekayode FO (2004). The use of manure to increase the yield and quality of amaranthus to feed rabbit in a humid tropical region. Journal of Animal and Veterinary Advances 3(11):758-762.

Akin-Idowu PE, Ademoyegun OT, Olagunju YO, Aduloju AO, Adebo UG (2017). Phytochemical content and antioxidant activity of five grain amaranth species. American Journal of Food Science and Technology 5(6):249-255.

Alonso-Miravalles L, O'Mahony JA (2018). Composition, protein profile and rheological properties of pseudocereal-based protein-rich ingredients. Foods 7(5):1-17.

Alpinar K, Özyürek M, Kolak U, Güçlü K, Aras Ç, Altun M, … Apak R (2009). Antioxidant capacities of some food plants wildly grown in Ayvalik of Turkey. Food Science and Technology Research 15(1):59-64.

Alvarez-Jubete L, Wijngaard H, Arendt EK, Gallagher E (2010). Polyphenol composition and in vitro antioxidant activity of amaranth, quinoa buckwheat and wheat as affected by sprouting and baking. Food Chemistry 119(2):770-778.

Apak R, Gorinstein S, Böhm V, Schaich Karen M, Özyürek M, Güçlü K (2013). Methods of measurement and evaluation of natural antioxidant capacity/activity (IUPAC Technical Report). Pure and Applied Chemistry 85(5):957-998.

Berger A, Monnard I, Dionisi F, Gumy D, Hayes KC, Lambelet P (2003). Cholesterol-lowering properties of amaranth flakes, crude and refined oils in hamsters. Food Chemistry 81(1):119-124.

Çekiç SD, Başkan KS, Tütem E, Apak R (2009). Modified cupric reducing antioxidant capacity (CUPRAC) assay for measuring the antioxidant capacities of thiol-containing proteins in admixture with polyphenols. Talanta 79(2):344-351.

Czerwinski J, Bartnikowska E, Leontowicz H, Lange E, Leontowicz M, Katrich E, … Gorinsteine S (2004). Oat (Avena sativa L.) and amaranth (Amaranthus hypochondriacus) meals positively affect plasma lipid profile in rats fed cholesterol-containing diets. The Journal of Nutritional Biochemistry 15(10):622-629.

Elbehri A, Putnam DH, Schmitt M (1993). Nitrogen fertilizer and cultivar effects on yield and nitrogen-use efficiency of grain amaranth. Agronomy Journal 85(1):120-128.

Gorinstein S, Pawelzik E, Delgado-Licon E, Haruenkit R, Weisz M, Trakhtenberg S (2002). Characterization of pseudocereal and cereal proteins by protein and amino acid analyses. Journal of the Science of Food and Agriculture 82(8):886-981.

Gorinstein S, Lojek A, Číž M, Pawelzik E, Delgado-Licon E, Medina OJ, … Goshev I (2008). Comparison of composition and antioxidant capacity of some cereals and pseudocereals. International Journal of Food Science & Technology 43(4):629-637.

Hlinková A, Bednárová A, Havrlentová M, Šupová J, Čičová I (2013). Evaluation of fatty acid composition among selected amaranth grains grown in two consecutive years. Biologia 68(4):641-650.

Huang D, Boxin Ou, Prior RL (2005). The chemistry behind antioxidant capacity assays. Journal of Agricultural and Food Chemistry 53(6):1841-1856.

Kim D-O, Jeong SW, Lee CY (2003). Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chemistry 81(3):321-326.

Law-Ogbomo KE, Ajayi SO (2009). Growth and yield performance of Amaranthus cruentus influenced by planting density and poultry manure application. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 37(1):195-199.

Lopez VR, Razzeto GS, Gimenez MS, Escudero NL (2011). Antioxidant properties of Amaranthus hypochondriacus seeds and their effect on the liver of alcohol-treated rats. Plant Foods for Human Nutrition 66(2):157-162.

Matuz J, Bartók T, Mórocz-Salamon K, Bóna L (2000). Structure and potential allergenic character of cereal proteins: I. Protein content and amino acid composition. Cereal Research Communications 28(3):263-270.

Martirosyan DM, Miroshnichenko LA, Kulakova SN, Pogojeva AV, Zoloedov VI (2007). Amaranth oil application for coronary heart disease and hypertension. Lipids in Health and Disease 6(1):1-12.

Mlakar SG, Jakop M, Turinek M, Robačer M, Bavec M, Franc Bavec (2012). Protein concentration and amino acid composition in grain amaranth (Amaranthus cruentus L.) as affected by sowing date and nitrogen fertilization. African Journal of Agricultural Research 7(37):5238-5246.

Nana FW, Hilou A, Millogo JF, Nacoulma OG (2012). Phytochemical composition, antioxidant and xanthine oxidase inhibitory activities of Amaranthus cruentus L. and Amaranthus hybridus L. extracts. Pharmaceuticals 5(6):613-628.

Okarter N (2012). Phenolic compounds from the insoluble-bound fraction of whole grains do not have any celular antioxidant activity. Live Science and Medicine Research 1:1-10.

Oyedeji S, Animasaun DA, Bello AA, Agboola OO (2014). Effect of NPK and poultry manure on growth, yield, and proximate composition of three amaranths. Journal of Botany 828750.

Park Y-S, Jung S-T, Kang S-G, Delgado-Licon E, Katrich E, Tashma Z, … Gorinstein S (2006). Effect of ethylene treatment on kiwifruit bioactivity. Plant Foods for Human Nutrition 61(3):151-156.

Paśko P, Bartoń H, Zagrodzki P, Gorinstein S, Fołta M, Zachwieja Z (2009). Anthocyanins, total polyphenols and antioxidant activity in amaranth and quinoa seeds and sprouts during their growth. Food Chemistry 115(3):994-998.

Paśko P, Sajewicz M, Gorinstein S, Zachwieja Z (2008). Analysis of selected phenolic acids and flavonoids in Amaranthus cruentus and Chenopodium quinoa seeds and sprouts. Acta Chromatographica 20(4):661-672.

Peiguo G, Al-Khatib K (2003). Temperature effects on germination and growth of redroot pigweed (Amaranthus retroflexus), palmer amaranth (Amaranthus palmeri), and common waterhemp (Amaranthus rudis). Weed Science 51(6):869-875.

Perales-Sánchez JXK, Reyes-Moreno C, Gómez-Favela MA, Milán-Carrillo J, Cuevas-Rodríguez EO, Valdez-Ortiz A, Gutiérrez-Dorado R (2014). Increasing the antioxidant activity, total phenolic and flavonoid contents by optimizing the germination conditions of amaranth seeds. Plant Foods for Human Nutrition 69(3):196-202.

Pospišil A, Pospišil M, Varga B, Svečnjak Z (2006). Grain yield and protein concentration of two amaranth species (Amaranthus spp.) as influenced by the nitrogen fertilization. European Journal of Agronomy 25(3):250-253.

Prakash D, Pal M (1991). Nutritional and antinutritional composition of vegetable and grain amaranth leaves. Journal of the Science of Food and Agriculture 57(4):573-583.

Rabie MA, Soliman AZ, Diaconeasa ZS, Bele C (2015). Effect of pasteurization and shelf life on the physicochemical properties of physalis (Physalis peruviana L.) juice. Journal of Food Processing and Preservation 39(6):1051-1060.

Ramakrishna A, Ravishankar GA (2011). Influence of abiotic stress signals on secondary metabolites in plants. Plant signaling and Behavior 6(11):1720-1731.

Repo-Carrasco-Valencia R, Hellström JK, Pihlava JM, Mattila PH (2010). Flavonoids and other phenolic compounds in Andean indigenous grains: quinoa (Chenopodium quinoa), kañiwa (Chenopodium pallidicaule) and kiwicha (Amaranthus caudatus). Food Chemistry 120(1):128-133.

Shamloo M, Babawale EA, Furtado A, Henry RJ, Eck PK, Jones PJH (2017). Effects of genotype and temperature on accumulation of plant secondary metabolites in Canadian and Australian wheat grown under controlled environments. Scientific Reports 7(1):1-13.

Singleton VL, Orthofer R, Lamuela-Raventós RM (1999). [14] Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology 299(152):152-178.

Steffensen SK, Rinnan Å, Mortensen AG, Laursen B, de Troiani RM, Noellemeyer EJ, … Fomsgaard IS (2011). Variations in the polyphenol content of seeds of field grown Amaranthus genotypes. Food Chemistry 129(1):131-138.

Vollmannová A, Margitanová E, Tóth T, Timoracká M, Urminská D, Bojňanská T, Čičová I (2013). Cultivar influence on total polyphenol and rutin contents and total antioxidant capacity in buckwheat, amaranth, and quinoa seeds. Czech Journal of Food Science 31(6):589-595.

Venskutonis PR, Kraujalis P (2013). Nutritional components of amaranth seeds and vegetables: a review on composition, properties, and uses. Comprehensive Reviews in Food Science and Food Safety 12(4):381-412.

Zhong Y, Shahidi F (2015). Methods for the assessment of antioxidant activity in foods. In: Handbook of Antioxidants for Food Preservation. Woodhead Publishing Series in Food Science, Technology and Nutrition pp 287-333.




How to Cite

STĂNILĂ, A., CIOANCA, B., DIACONEASA, Z., STĂNILĂ, S., SIMA, N., & SIMA, R. M. (2019). Phytochemical Composition and Antioxidant Activity of Various Grain Amaranth Cultivars. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47(4), 1153–1160.



Research Articles
DOI: 10.15835/nbha47411714

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