Genetic Diversity of some Daffodil (Narcissus L. spp.) Genotypes in Turkey by Using SRAP Markers

  • Emrah ZEYBEKOĞLU University of Ege, Faculty of Agriculture, Department of Horticulture, Bornova, Izmir
  • Tansal KAYGISIZ ASCIOGUL University of Ege, Faculty of Agriculture, Department of Horticulture, Bornova, Izmir
  • Hulya ILBI University of Ege, Faculty of Agriculture, Department of Horticulture, Bornova, Izmir
  • Mustafa E. OZZAMBAK University of Ege, Faculty of Agriculture, Department of Horticulture, Bornova, Izmir
Keywords: flower bulb; genetic relationships; populations; SRAP

Abstract

Daffodil (Narcissus L. spp.) is one of the oldest ornamental flowers that have been cultivated from ancient times to nowadays. The species Narcissus tazetta L. and N. serotinus L. have spread out in different regions of Turkey and some other species have been naturalized. The purpose of this study was to characterize some daffodil populations that were collected from different regions of Turkey by using SRAP markers. Twenty-two daffodil populations of different species such as N. tazetta, N. serotinus, N. pseudonarcissus L., N. poeticus L. and N. jonquilla L. were used. Thirty-seven of 48 SRAP primer combinations showed scorable and polymorphic bands between samples. The similarity coefficients were used to construct a dendrogram by Unweighted Pair-Group Method with Arithmetic Averages (UPGMA) using NTSYS-PC. Cophenetic correlation between ultrametric similarities of tree and similarity matrix were high (r = 0.97). The genotypes of Narcissus species were divided into 2 main groups. Although there was less genetic variation among N. tazetta species, even collected from different regions of Turkey, N. poeticus, N. pseudonarsis, N. jonquilla and N. serotinus had more genetic divergence among them. It can be concluded that genetic variation of Narcissus species can be used for daffodil breeding programs.

Metrics

Metrics Loading ...

References

Barrett SCH, Cole WW, Herrera CM (2004). Mating patterns and genetic diversity in the wild daffodil Narcissus longispathus (Amaryllidaceae). Heredity 92(5):459-465.

Baytop T, Mathew B (1984). The bulbous plants of Turkey. BT Batsforf Ltd. London.

Davis PH (1984). Flora of Turkey and the East Aegean Islands. Volume 8. Edinburgh University Press, Edinburgh.

Dice LR (1945). Measures of the amount of ecologic association between species. Ecology 26(3):297-302.

Doorenbos J (1954). Notes on the history of bulb breeding in the Netherlands. Euphytica 3(1):1-8.

Doyle JJ, Doyle JL (1990). Isolation of plant DNA from fresh tissue. Focus 12(13):13-15.

Ferriol M, Pico B, Nuez F (2003). Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers. Theoretical and Applied Genetics 107(2):271-282.

Graham SW, Barrett SCH (2004). Phylogenetic reconstruction of the evolution of stylar polymorphisms in Narcissus (Amaryllidaceae). American Journal of Botany 91(7):1007-1021.

Jimenez JF, Sanchez-Gomez P, Guerra J, Molins A, Rosello JA (2009). Regional speciation or taxonomic inflation? The status of several narrowly distributed and endangered species of Narcissus using ISSR and nuclear ribosomal ITS markers. Folia Geobotanica 44(2):145-158.

Li G, Quiros CF (2001). Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theoretical and Applied Genetics 103(2-3):455-461.

Li G, Gao M, Yang B, Quiros CF (2003). Gene for gene alignment between the Brassica and Arabidopsis genomes bydirect transcriptome mapping. Theoretical and Applied Genetics 107(1):168-180.

Mathew B (2002). Classification of the genus Narcissus. Narcissus and Daffodil. Hanks GR (Ed). Taylor & Francis pp 30-52.

Nunez DR, De Castro CO, Ruiz SR, Ariza FA (2003). The origin of cultivation and wild ancestors of daffodils (Narcissus subgenus Ajax) (Amaryllidaceae) from an analysis of early illustrations. Scientia Horticulturae 98(4):307-330.

Perez-Barrales R, Vargas P, Arroyo J (2003). Convergent evolution of flower polymorphism in Narcissus (Amaryllidaceae). New Phytologist 161(1):235-252.

Rohlf FJ (2000). NTSYS-pc, numerical taxonomy and multivariate analysis system, version 2.11. New York, Exeter, Setauket.

Tucci GF, Winfield MO, D’amato GF, Gregori C, Trombetta B, De Dominicis RI (2004). Genetic diversity in Narcissus poeticus L. and N. radiiflorus Salisb. (Amaryllidaceae) in two different populations: AFLP and karyological studies. Caryologia 57(4):405-411.

Upadhyaya HD, Bramel PJ, Ortiz R, Sing, S (2002). Geographical patterns of diversity for morphological and agronomic traits in the groundnut germplasm collection. Euphytica 128(2):191-204.

Wylie AP (1952). The history of the garden Narcissi. Heredity 6(2):137-156.

Published
2019-12-05
How to Cite
ZEYBEKOĞLU, E., KAYGISIZ ASCIOGUL, T., ILBI, H., & OZZAMBAK, M. E. (2019). Genetic Diversity of some Daffodil (Narcissus L. spp.) Genotypes in Turkey by Using SRAP Markers . Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47(4), 1293-1298. https://doi.org/10.15835/nbha47411664
Section
Research Articles