Seed Treatment Techniques to Improve Germination of Wild Asparagus (Asparagus acutifolius L.), a Potential New Crop


  • Nikolaos KATSENIOS Agricultural University of Athens, Department of Crop Science, Laboratory of Agronomy, 75 Iera Odos Str., 11855 Athens (GR)
  • Ioannis E. ROUSSIS Agricultural University of Athens, Department of Crop Science, Laboratory of Agronomy, 75 Iera Odos Str., 11855 Athens (GR)
  • Aspasia EFTHIMIADOU Hellenic Agricultural Organization-Demeter, Institute of Soil and Water Resources, Department of Soil Science of Athens, Lycovrisi (GR)
  • Ioanna KAKABOUKI Agricultural University of Athens, Department of Crop Science, Laboratory of Agronomy, 75 Iera Odos Str., 11855 Athens (GR)
  • Dimitrios BILALIS Agricultural University of Athens, Department of Crop Science, Laboratory of Agronomy, 75 Iera Odos Str., 11855 Athens (GR)



Asparagus acutifolius; germination; scarification; seed dormancy; stratification


Pre-sowing seed treatment techniques of stratification and scarification were used in order to find the most appropriate method to overcome dormancy of wild asparagus (Asparagus acutifolius L.) seeds. An indoor and an outdoor experiment were carried out at the Agricultural University of Athens. For the indoor experiment, the pre-sowing treatments of stratification (fresh and stratified seeds) and scarification (non-scarified, mechanical scarification and chemical scarification with sulfuric acid) were used as the main treatments, and three different temperatures (15, 20 and 25 °C) as sub-treatments. For the outdoor experiment, there were only the treatments of stratification and scarification. The stratification of asparagus seeds was found to have a positive effect on germination and vigor indices compared to non-stratified (fresh) seeds. The highest germination percentage, vigor index I and II were recorded with the stratified seeds that had been mechanically scarified in both indoor and outdoor conditions. The results indicate that just the scarification cannot improve germination of fresh collected seeds of wild asparagus. Stratification increased the germination percentage from 2.7-6.6% to 45.1-75.3%. The mechanical and the chemical scarification had a positive effect on the root length compared to the non-scarified seeds that have been stratified. These pre-sowing treatments can increase the germination percentage and produce vigorous seedlings that can be used to establish plantations of this potential new crop.


Abdul-Baki AA, Anderson JD (1973). Vigor determination in soybean by multiple criteria. Crop Science 13(6):630-633.

Akoumianaki-Ioannidou A, Gerasimidou E, Salta A, Roussis I, Bilalis D (2019). Sexual and vegetative propagation of Hypericum empetrifolium Willd. subsp. empetrifolium. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 47(2):282-287.

Benincasa P, Tei F, Rosati A (2007). Plant density and genotype effects on wild asparagus (Asparagus acutifolius L.) spear yield and quality. HortScience 42(5):1163-1166.

Bilalis D, Katsenios N, Efthimiadou A, Karkanis A, Efthimiadis P (2012). Investigation of pulsed electromagnetic field as a novel organic pre-sowing method on germination and initial growth stages of cotton. Electromagnetic Biology and Medicine 31(2):143-150.

Bilusic T, Sola I, Rusak G, Poljuha D, Culic VC (2019). Antiproliferative and pro-apoptotic activities of wild asparagus (Asparagus acutifolius L.), black bryony (Tamus communis L.) and butcher’s broom (Ruscus aculeatus L.) aqueous extracts against T24 and A549 cancer cell lines. Journal of Food Biochemistry 43(4):e12781.

Boubetra K, Amirouche N, Amirouche R (2017). Comparative morphological and cytogenetic study of five Asparagus (Asparagaceae) species from Algeria including the endemic A. altissimus Munby. Turkish Journal of Botany 41(6):588-599.

Conversa G, Elia A (2009). Effect of seed age, stratification, and soaking on germination of wild asparagus (Asparagus acutifolius L.). Scientia Horticulturae 119(3):241-245.

Conversa G, Lazzizera C, Elia A (2010). Effects of after-ripening, stratification and GA3 on dormancy release and on germination of wild asparagus (Asparagus acutifolius L.) seeds. Scientia Horticulturae 125(3):196-202.

Di Maro A, Pacifico S, Fiorentino A, Galasso S, Gallicchio M, Guida V,… Parente A (2013). Raviscanina wild asparagus (Asparagus acutifolius L.): A nutritionally valuable crop with antioxidant and antiproliferative properties. Food Research International 53(1):180-188.

Efthimiadou A, Karkanis A, Bilialis D, Katsenios N (2012). Review: Cultivation of cow cockle (Vaccaria hispanica (Mill.) Rauschert): an industrial-medicinal weed. Industrial Crops and Products 40:307-311.

Ferrara L, Dosi R, Di Maro A, Guida V, Cefarelli G, Pacifico S, … Parente A (2011). Nutritional values, metabolic profile and radical scavenging capacities of wild asparagus (Asparagus acutifolius L.). Journal of Food Composition and Analysis 24(3):326-333.

García-Herrera P, Cortes Sánchez-Mata M, Cámara M, Tardío J, Olmedilla-Alonso B (2013). Carotenoid content of wild edible young shoots traditionally consumed in Spain (Asparagus acutifolius L., Humulus lupulus L., Bryonia dioica Jacq. and Tamus communis L.). Journal of the Science of Food and Agriculture 93(7):1692-1698.

Guida V, Cantarella M, Chambery A, Mezzacapo MC, Parente A, Landi N, … Di Maro A (2014). Purification and characterization of novel cationic peroxidases from Asparagus acutifolius L. with biotechnological applications. Molecular Biotechnology 56(8):738-746.

Gupta S, Kumar A, Sharma SN (2002). Improvement of seed germination in Asparagus racemosus Willd. Journal of Herbs, Spices & Medicinal Plants 9(1):3-9.

Hartmann HT, Kester DE, Davies FT, Geneve RL (2011). Hartmann & Kester’s Plant Propagation. Principles and Practices (8th Edition), Prentice-Hall, USA.

Karaguzel O, Cakmakci S, Ortacesme V, Aydinoglu B (2004). Influence of seed coat treatments on germination and early seedling growth of Lupinus varius L. Pakistan Journal of Botany 36(1):65-74.

Kaska A, Deniz N, Mammadov R. (2018). Biological activities of wild asparagus (Asparagus acutifolius L.). International Journal of Secondary Metabolite 5(3):243-251.

Kasture S, Kasture A, Ballero M, Maxia A (2009). Antioxidant, anti-inflammatory, and adaptogenic activity of Asparagus acutifolius extract. Oriental Pharmacy and Experimental Medicine 9(1):83-89.

Katsenios N, Bilalis D, Efthimiadou A, Aivalakis G, Nikolopoulou AE, Karkanis A, Travlos I (2016). Role of pulsed electromagnetic field on enzyme activity, germination, plant growth and yield of durum wheat. Biocatalysis and Agricultural Biotechnology 6:152-158.

Kokko EG, Volkmar KM, Gowen BE, Entz T (1993). Determination of total root surface area in soil core samples by image analysis. Soil and Tillage Research 26(1):33-43.

Martins D, Barros L, Carvalho AM, Ferreira ICFR (2011). Nutritional and in vitro antioxidant properties of edible wild greens in Iberian Peninsula traditional diet. Food Chemistry 125(2):488-494.

Molina M, Pardo-de-Santayana M, García E, Aceituno-Mata L, Morales R, Tardío J (2012). Exploring the potential of wild food resources in the Mediterranean region: natural yield and gathering pressure of the wild asparagus (Asparagus acutifolius L.). Spanish Journal of Agricultural Research 10(4):1090-1100.

Molina M, Pardo-de-Santayana M, Tardío J (2016). Natural production and cultivation of Mediterranean wild edibles. In: Sánchez-Mata M, Tardío J (Eds). Mediterranean wild edible plants. Springer, New York pp 81-107.

Sánchez-Mata M., Matallana-González M., Morales P (2016). The contribution of wild plants to dietary intakes of micronutrients (I): Vitamins. In: Sánchez-Mata M, Tardío J (Eds) Mediterranean Wild Edible Plants. Springer, New York pp 111-139.

Shanmugavalli M, Renganayaki PR, Menaka C (2007). Seed dormancy and germination improvement treatments in fodder sorghum. International Crops Research Institute Semi-Arid Tropics 3:1-3.

Traveset A, Riera N, Mas RE (2001). Passage through bird guts causes interspecific differences in seed germination characteristics. Functional Ecology 15(5):669-675.




How to Cite

KATSENIOS, N., ROUSSIS, I. E., EFTHIMIADOU, A., KAKABOUKI, I., & BILALIS, D. (2019). Seed Treatment Techniques to Improve Germination of Wild Asparagus (Asparagus acutifolius L.), a Potential New Crop. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47(3), 995–1000.



DOI: 10.15835/nbha47311554

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