Effect of Irrigation and Fertilization Levels on Mineral Composition of Cannabis sativa L. Leaves


  • Eleni WOGIATZI Technological Educational Institute of Thessaly, Department of Agronomy Technology, 41100 Larissa (GR)
  • Nikolaos GOUGOULIAS Technological Educational Institute of Thessaly, Department of Agronomy Technology, 41100 Larissa (GR)
  • Kyriakos D. GIANNOULIS Technological Educational Institute of Thessaly, Department of Agronomy Technology, 41100 Larissa; University of Thessaly, Department of Agriculture, Crop Production and Rural Environment, Laboratory of Agronomy and Applied Crop Physiology, Fytokoy St., 38446 Volos (GR)
  • Christina-Anna KAMVOUKOU Technological Educational Institute of Thessaly, Department of Agronomy Technology, 41100 Larissa; Aristotle University of Thessaloniki, School of Pharmacy (GR)




cannabis; fertilization; irrigation; mineral composition; nutrient uptake


A field experiment was conducted in central Greece to study the effect of two irrigation (I1: 100% ETo, I2: 60% ETo) and N-fertilization levels (N1: 244, N2: 184 kg ha-1), on the nutrients concentration of Cannabis sativa leaves (cv. ‘Fibranova’). The  N, K, Ca, Mg, P -concentration in the leaves was ranged by 2.8 to 3.51%, 1.8 to 2.57%, 1.96 to 2.17%, 0.86 to 0.88%, and 0.3 to 0.37% respectively, while by the micronutrients the iron showed the highest concentration that ranged by 129 to 139.8 mg kg-1dw. The treatment I1F1, where the highest level of irrigation and N -fertilization was applied, compared to the other treatments, showed the highest dry biomass yield, however, in the leaves the highest concentrations of N, K, Mn and Cu were not observed. Moreover, it was found that the N, K, Ca, Mg, P and Fe removal only by one ton dry biomass of leaves was ranged by 28 to 35.12 kg, 18.01 to 25.65 kg, 19.6 to 21.7 kg, 8.34 to 8.75 kg, 3.01 to 3.70 kg and 0.129 to 0.140 kg, respectively. These results could contribute optimal fertilizer application and therefore to the reduction of production costs of the crop.


Amaducci S, Colauzzi M, Bellocchi G, Cosentino SL, Pahkala K, Stomph TJ, Venturi G (2012). Evaluation of a phenological model for strategic decisions for hemp (Cannabis sativa L.) biomass production across European sites. Industrial Crops and Products 37(1):100-110.

Angelini LG, Tavarini S, Cestone B, Beni C (2014).Variation in mineral composition in three different plant organs of five fibre hemp (Cannabis sativa L.) cultivars. Agrochimica 58(1):1-18.

Benner BL, Bazzaz FA (1988). Carbon and mineral element accumulation and allocation in two annual plant species in response to timing of nutrient addition. The Journal of Ecology 76(1):19-40.

Bertoli A, Tozzi S, Pistelli L, Angelini L G (2010). Fiber hemp inflorescences: From crop-residues to essential oil production. Industrial Crops and Products 32(3):329-337.

Bocsa I, Karus M (1998). The cultivation of hemp: botany, varieties, cultivation and harvesting. Hemptech.Sebastopol, CA, USA.

Bouloc P, Van Der Werf HMG (2013). The role of hemp in sustainable development. Hemp: In: Bouloc P, Allegret S, Arnaud L (Eds). Industrial Production and Uses pp 278-289.

Bouyoucos GJ (1962). Hydrometer method improved for making particle size analysis of soils. Agronomy Journal 54(5):464-465.

Cosentino SL, Testa G, Scordia D, Copani V (2012). Sowing time and prediction of flowering of different hemp (Cannabis sativa L.) genotypes in southern Europe. Industrial Crops and Products 37(1):20-33.

Finnan J, Styles D (2013). Hemp: a more sustainable annual energy crop for climate and energy policy. Energy Policy 58:152-162.

Ghani A, Saeed S, Ali Z, Ahmad I, Ishtiaq M (2012). Heavy metals and nutritional composition of some selected herbal plants of Soon Valley, Khushab, Punjab, Pakistan. African Journal of Biotechnology 11(76):14064-14068.

Hakala K, Keskitalo M, Eriksson C, Pitkänen T (2009). Nutrient uptake and biomass accumulation for eleven different field crops. Agricultural and Food Science 18:366-387.

Ivanyi I (2011). Relationship between leaf nutrient concentration and the yield of fiber hemp (Cannabis sativa L.). Research Journal of Agricultural Science 43(3):70-76.

Jones Jr JB, Case VW (1990). Sampling, handling and analyzing plant tissue samples. Sampling, handling and analyzing plant tissue samples. Ed. 3, pp 389-427.

Jones Jr LJB (1998). Plant nutrition manual CRC Press. New York.

Mengel K, Kirkby EA, Kosegarten H, Appel T (2001). Principles of plant nutrition. Kluwer Academic Publishers. Dordrecht/Boston/London, pp 464-469.

Mihoc M, Pop G, Alexa E, Radulov I (2012). Nutritive quality of Romanian hemp varieties (Cannabis sativa L.) with special focus on oil and metal contents of seeds. Chemistry Central Journal 6(1):122.

Page AL, Miller RH, Keeney DR (1982). Methods of soil analysis. Part 2: Chemical and microbiological properties. Agronomy, ASA and SSSA, Madison,Wisconsin, USA.

Ryan BF, Joiner BL, Cryer JD (2005). MINITAB Handbook: Updated for release 14, 5th edition. Brooks/Cole-Thomson Learning Inc., Kentaky, KY.

Small E, Marcus D (2002). Hemp: a new crop with new uses for North America. Trends in New Crops and New Uses 284-326.

Small E, Marcus D (2003). Tetrahydrocannabinol levels in hemp (Cannabis sativa L.) germplasm resources. Economic Botany 57(4):545-558.

Struik PC, Amaducci S, Bullard MJ, Stutterheim NC, Venturi G, Cromack HTH (2000). Agronomy of fiber hemp (Cannabis sativa L.) in Europe. Industrial Crops and Products 11(2-3):107-118.

Tang K, Struik PC, Yin X, Thouminot C, Bjelková M, Stramkale V, Amaducci S (2016). Comparing hemp (Cannabis sativa L.) cultivars for dual-purpose production under contrasting environments. Industrial Crops and Products 87:33-44.

Van der Werf HM, Turunen L (2008). The environmental impacts of the production of hemp and flax textile yarn. Industrial Crops and Products 27(1):1-10.

Varian M (1989). Flama atomic absorption spectroscopy. Analytical Methods. Varian Australia. Publ. No: 85-100009-00.

Zatta A, Monti A, Venturi G (2012). Eighty years of studies on industrial hemp in the Po Valley (1930-2010). Journal of Natural Fibers 9(3):180-196.

Zerihun A, Chandravanshi BS, Debebe A, Mehari B (2015). Levels of selected metals in leaves of Cannabis sativa L. cultivated in Ethiopia. SpringerPlus 4(1): 359.




How to Cite

WOGIATZI, E., GOUGOULIAS, N., GIANNOULIS, K. D., & KAMVOUKOU, C.-A. . (2019). Effect of Irrigation and Fertilization Levels on Mineral Composition of Cannabis sativa L. Leaves. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47(4), 1073–1080. https://doi.org/10.15835/nbha47411527



Research Articles
DOI: 10.15835/nbha47411527