Growth, Photosynthetic Pigments, Phenolic, Glucosinolates Content and Antioxidant Capacity of Broccoli Sprouts in Response to Nanoselenium Particles Supply

  • Simona Ioana VICAS University of Oradea, Faculty of Environmental Protection, 26 Gen. Magheru St., 410048, Oradea http://orcid.org/0000-0002-8944-9458
  • Simona CAVALU University of Oradea, Faculty of Medicine and Pharmacy, 10 P-ta 1 Decembrie, 410087, Oradea
  • Vasile LASLO University of Oradea, Faculty of Environmental Protection, 26 Gen. Magheru St., 410048, Oradea
  • Mariana TOCAI University of Oradea, Biomedical Sciences Doctoral School, 1 University St., 410087, Oradea
  • Traian O. COSTEA University of Oradea, Industrial Engineering Doctoral School, 1 University St., 410087, Oradea
  • Liviu MOLDOVAN University of Oradea, Faculty of Electrical Engineering and Information Technology, 1 University St., 410087, Oradea
Keywords: Brassica oleracea; DPPH; FRAP; FTIR; glucosinolates; HPLC; hyperspectral microscopy; selenium

Abstract

Improving the nutritional quality of plants has emerged from the fact that macro- and micro- nutrients are limited in various agricultural areas. The aim of our study was the biofortification of broccoli sprouts with selenium nanoparticles (NSePs) and evaluation of growth parameters, assimilator pigments content, total phenols, glucosinolates content along with antioxidant capacity, in order to boost value added output, such as improved nutrition and food functionality. NSePs were prepared by reduction of NaHSeO3 using glucose as reducing agent, and characterized from structural and morphological point of view. The growth of broccoli seedlings was dependent on NSePs concentration. The treatment with 10 and 50 ppm NSePs caused a slight increase in total biomass, by contrast with 100 ppm treatment. Chlorophyll content, total carotenoid and total phenols content was not affected by the treatment of broccoli sprouts with different concentrations of NSePs. Instead, the content of individual glucosinolates varied between the samples, depending on the levels of NSePs. The highest antioxidant capacity was obtained for 100 ppm NSePs concentration. The effective uptake of NSePs was further demonstrated by FTIR spectroscopy and Hyperspectral Microscopy. NSePs did not induce any toxicity on broccoli sprouts. Moreover, broccoli supply with NSePs may target higher nutritional impact and health benefits.

Metrics

Metrics Loading ...

References

Abdulah R, Faried A, Kobayashi K, Yamazaki C, Suradji EW, Ito K, … Koyama H (2009). Selenium enrichment of broccoli sprout extract increases chemo sensitivity and apoptosis of LNCaP prostate cancer cells. BMC Cancer 9(1):414.

Ávila FW, Yang Y, Faquin V, Ramos SJ, Guilherme LRG, Theodore W, … Li L (2014). Impact of selenium supply on Se-methylselenocysteine and glucosinolate accumulation in selenium-biofortified Brassica sprouts. Food Chemistry 165:578-586.

Bachiega P, Salgado JM, Ernesto de Carvalho J, Ruiz ALTG, Schwarz K, Tezotto T, Morzelle MC (2016). Antioxidant and antiproliferative activities in different maturation stages of broccoli (Brassica oleracea Italica) biofortified with selenium. Food Chemistry 190:771-776.

Barbieri G, Pernice R, Maggio A, De Pascale S, Fogliano V (2008). Glucosinolates profile of Brassica rapa L. subsp. sylvestris L. Janch. var. esculenta. Hort. Food Chemistry 107(4):1687-1691.

Barickman TC, Kopsell DA, Sams CE (2014). Impact of selenium fertilization on glucosinolate concentration in Arabidopsis thaliana and rapid cycling Brassica oleracea. Journal of Plant Nutrition 37(3):343-356.

Bassem MR, Samir WA, Mahmoud HK (2012). Met-hemoglobin concentration reduction and methemoglobin reductase enhancement after applying of α-Tocopherol and selenium combination as a radio protector against ionizing radiation exposure. Wulfenia Journal 19(10):373-389.

Benzie IF, Strain JJ (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochemistry 239(1):70-76.

Brand-Williams W, Cuvelier ME, Berset C (1995). Use of free radical method to evaluate antioxidant activity. LWT-Food Science and Technology 28(1):25-30.

Cavalu S, Prokisch J, Laslo V, Vicas S (2017). Preparation, Structural Characterisation and Release Study of Novel Hybrid Microspheres Entrapping Nano-Selenium, Produced by Green Synthesis. IET Nanobiotechnology 11 (4):426 -432 .

Cavalu S, Antoniac IV, Fritea L, Mates IM, Milea C, Laslo V, Vicas S, Mohan A (2018). Surface modifications of the titanium mesh for cranioplasty using selenium nanoparticles coating. Journal of Adhesion Science and Technology 32 (22):2509-2522.

Da Luz BR (2006). Attenuated total reflectance spectroscopy of plant leaves: a tool for ecological and botanical studies. New Phytologist 172(2):305-318.

Ducsay L, Ložek O (2006). Effect of selenium foliar application on its content in winter wheat grain. Plant, Soil and Environment 52(2):78-82.

El-Ramady H, Abdalla N, Taha HS, Alshaal T, El-Henawy A, Faizy SE-DA, … Fári M. (2015). Se and nano-Se in plant nutrition. Environmental Chemistry Letters 14(1):123-147.

Hassanin KMA, El-Kawi SHA, Hashem KS (2013). The prospective protective effect of selenium nanoparticles against chromium-induced oxidative and cellular damage in rat thyroid. International Journal of Nanomedicine 8:1713-1720.

Huang B, Zhang J, Hou J, Chen C (2003). Free radical scavenging efficiency of nano-Se in vitro. Free Radicals in Biology and Medicine 35(7):805-813.

Islam MZ, Mele MA, Baek JP, Kang H-M (2018). Iron, iodine and selenium effects on quality, shelf life and microbial activity of cherry tomatoes. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 46(2):388-392.

Jiang Y, Zeng ZH, Bu Y, Ren CZ, Li JZ, Han JJ, … Hu YG (2015). Effects of selenium fertilizer on grain yield, Se uptake and distribution in common buckwheat (Fagopyrum esculentum Moench). Plant, Soil and Environment 61(8):371-377.

Khoei NS, Lampis S, Zonaro E, Yrjälä K, Bernardi P, Vallini G (2017). Insights into selenite reduction and biogenesis of elemental selenium nanoparticles by two environmental isolates of Burkholderia fungorum. New Biotechnology 34:1-11.

Piekarska A, Kołodziejski D, Pilipczuk T, Bodnar M, Konieczka P, Kusznierewicz B, … Bartoszek A (2014). The influence of selenium addition during germination of Brassica seeds on health-promoting potential of sprouts. International Journal of Food Science and Nutrition 65(6):692-702.

Ramos SJ, Faquin V, Guilherme LRG, Castro EM, Ávila FW, Carvalho GS, Bastos CEA, Oliveira C (2010). Selenium bio fortification and antioxidant activity in lettuce plants fed with selenate and selenite. Plant, Soil and Environment 56(12):584-588.

Reilly C (2006). Selenium in food and health. 2nd ed., Springer, US, New York.

Rychlik J, Olejnik A, Olkowicz M, Kowalska K, Juzwa W, Myszka K, … Grajek W (2015). Antioxidant capacity of broccoli sprouts subjected to gastrointestinal digestion. Journal of Science of Food and Agriculture 95(9):1892-1902.

Sajedi N, Madani H, Naderi A (2011). Effect of microelements and selenium on superoxide dismutase enzyme, malondialdehyde activity and grain yield maize (Zea mays L.) under water deficit stress. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 39(2):153-159.

Sharma KD, Stähler K, Smith B, Melton L (2011). Antioxidant capacity, polyphenolics and pigments of broccoli-cheese powder blends. Journal of Food Science Technology 48(4):510-514.

Singleton VL, Orthofer R, Lamuela-Raventos RM (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymology 299:152-178.

Štolfa I, Velki M, Vuković R, Ečimović S, Katanić Z, Lončarić Z (2017). Effect of different forms of selenium on the plant-soil-earthworm system. Journal of Plant Nutrition and Soil Science 180(2):231-240.

Taran N, Batsmanova L, Kovalenko M, Okanenko A (2016). Impact of metal nanoform colloidal solution on the adaptive potential of plants. Nanoscale Research Letters 11(1):1-6.

Tarasevičienė Ž, Danilčenko H, Jarienė E, Paulauskienė A, Marek Gajewski M (2009). Changes in some chemical components during germination of broccoli seeds. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 37(2):173-176.

Tian Q, Rosselot RA, Schwartz SJ (2005). Quantitative determination of intact glucosinolates in broccoli, broccoli sprouts, Brussels sprouts, and cauliflower by high-performance liquid chromatography–electrospray ionization–tandem mass spectrometry. Analytical Biochemistry 343(1):93-99.

Trolove SN, Tan Y, Morrison SC, Feng L, Eason J (2018). Development of a method for producing selenium-enriched radish sprouts. LWT-Food Science and Technology 95:187-192.

Vicas SI, Teusdea A, Carbunar M, Socaci S, Socaciu C (2013). Glucosinolates profile and antioxidant capacity of Romanian brassica vegetables obtained by organic and conventional agricultural practices. Plant Foods for Human Nutrition 68(3):313-321.

Zhang J, Taylor EW, Wan X, Peng D (2012). Impact of heat treatment on size, structure and bioactivity of elemental selenium nanoparticles. International Journal of Nanomedicine 7: 815-825.

Zhang J, Wang X, Xu T (2008). Elemental selenium as a potential chemopreventive agent with reduced risk of selenium toxicity: comparison with methylselenocysteine in mice. Toxicology Science 101:22-31.

Published
2019-06-18
How to Cite
VICAS, S. I., CAVALU, S., LASLO, V., TOCAI, M., COSTEA, T. O., & MOLDOVAN, L. (2019). Growth, Photosynthetic Pigments, Phenolic, Glucosinolates Content and Antioxidant Capacity of Broccoli Sprouts in Response to Nanoselenium Particles Supply. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47(3), 821-828. https://doi.org/10.15835/nbha47311490
Section
Research Articles