High-Density Kinetic Analysis of the Metabolomic and Transcriptomic Response of Ginkgo biloba Flavonoids Biosynthesis to Selenium Treatments

  • Linling LI Wuhan Polytechnic University, College of Biology and Pharmaceutical Engineering, Wuhan, 430023
  • Jie YU Economic Forest Germplasm Improvements and Comprehensive Utilization of Resources of Hubei Key Laboratories, Hubei Huanggang, 438000
  • Honghui YUAN Economic Forest Germplasm Improvements and Comprehensive Utilization of Resources of Hubei Key Laboratories, Hubei Huanggang, 438000
  • Sanxing ZHA Wuhan Polytechnic University, College of Biology and Pharmaceutical Engineering, Wuhan, 430023
  • Kun DENG Wuhan Polytechnic University, College of Biology and Pharmaceutical Engineering, Wuhan, 430023
  • Xian XIAO Wuhan Polytechnic University, College of Biology and Pharmaceutical Engineering, Wuhan, 430023
  • Yanyan LUO Wuhan Polytechnic University, College of Biology and Pharmaceutical Engineering, Wuhan, 430023
  • Shuiyuan CHENG Wuhan Polytechnic University, College of Biology and Pharmaceutical Engineering, Wuhan, 430023
  • Hua CHENG Economic Forest Germplasm Improvement and Comprehensive Utilization of Resources of Hubei Key Laboratories, Hubei Huanggang, 438000
Keywords: flavonoids; foliage dressing; Gingko biloba; metabolome; sodium selenite; transcriptome

Abstract

As one of the rare and precious wood species since the ancient times, Gingko is also known as “living fossil”, which is a special plant resource of China. Gingko leaves, containing rich flavonoids, are valued with great medicinal significances. This paper treated Ginkgo seedlings by exogenous Sodium selenite (SS) in two ways: Foliage dressing (FD) and Root application (RA). Then transcriptome sequencing and metabolome test are performed. Results show that external SS has significant influence on the related gene expression level of flavonoids synthesis ways of Gingko, the FD can significantly induce gene expressions as CHS, FLS, FOMT, PAL, MYB1 and MYB2, and RA can significantly induce gene expressions as FOMT, MYB1 and MYB2. Compared with the control group, FA selenium application can help to accumulation of flavonoids, flavonols, flavonoids-C and isoflavones, especially quercetin and kaempferol that had a remarkable increase. This proved that a proper concentration of inorganic SS could promote the synthesis and accumulation of flavonoids in Gingko. qRT-PCR analysis also depicts that leaves treatment of sodium selenite can remarkably enhance the gene expression of CHS, FLS, FOMT and PAL, and RA selenium application can induce the gene expression of FLS and FOMT, but restrain the gene expression of CHS and PAL. Through the ways of FD and RA selenium application, this paper basically studied the regulatory effect of SS on ginkgo flavonoids synthesis and has laid a theoretical basis to improve flavonoids content in Ginkgo leaves through cultivation control means.

 

*********

 

In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.

 

*********

Metrics

Metrics Loading ...

References

Beek TAV, Montoro P (2009). Chemical analysis and quality control of Ginkgo biloba leaves, extracts, and phytopharmaceuticals. Journal of Chromatography A 1216(11):2002-2032.

Beek TAV, Montoro P (2009). Chemical analysis and quality control of Ginkgo biloba leaves, extracts, and phytopharmaceuticals. Journal of Chromatography A 1216(11):2002-2032.

Bilia AR (2002). Ginkgo biloba L. Fitoterapia 73(3):276-279.

Chen W, Gong L, Guo Z, Wang W, Zhang H, Liu X, … Luo J (2013). A novel integrated method tor large-scale detection, identification, and quantification of widely targeted metabolites: application in the study of rice metabolomics. Molecular Plant 6(6):1769-1780.

Chen D, Sun S, Cai D, Kong G (2017). Induction of mitochondrial-dependent apoptosis in T24 cells by a selenium (Se)-containing polysaccharide from Ginkgo biloba L. leaves. International Journal of Biological Macromolecules 101:126-130.

Chen J (2008). Ginkgo physiological growth change response to selenium. Nanjing Forestry University, Place Nanjing Forestry University Published.

Cheng H, Li L, Cheng S, Cao F, Wang Y, Yuan H (2011). Molecular cloning and function assay of a chalcone isomerase gene (GbCHI) from Ginkgo biloba. Plant Cell Reports 30(1):49-62.

Cheng H, Li L, Feng X, Yan W, Yuan H, Wu C, … Wang Y (2013a). Expression patterns of an isoflavone reductase-like gene and its possible roles in secondary metabolism in Ginkgo biloba. Plant Cell Reports 32(5):637-650.

Cheng H, Linling L, Shuiyuan C, Fuliang C, Feng X, Honghui Y, Conghua W (2013b). Molecular cloning and characterization of three genes encoding dihydroflavonol-4-reductase from Ginkgo biloba in anthocyanin biosynthetic pathway. PloS One 8:e72017.

Feng X, Cheng SY, Zhu J, Zhang WW, Yan W (2011). Effects of 5-aminolevulinic acid on chlorophyll, photosynthesis, soluble sugar and flavonoids of Ginkgo biloba. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 39(1):41-47.

Fischer TC, Gosch C, Pfeiffer J, Halbwirth H, Halle C, Stich K, Forkmann G (2007). Flavonoid genes of pear (Pyrus communis). Trees 21(5):521-529.

Grabherr MG, Haas BJ, Yassour M, Levin JZ, Thompson DA, Amit I, … Zeng Q (2011). Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nature Biotechnology 29(7):644.

Hahlbrock K, Scheel D (1989). Physiology and molecular biology of phenylpropanoid metabolism. Annual Review of Plant Biology 40(1):347-369.

Hua C, Linling L, Feng X, Yan W, Honghui Y, Conghua W, … Yuping W (2013). Expression patterns of an isoflavone reductase-like gene and its possible roles in secondary metabolism in Ginkgo biloba. Plant Cell Reports 32(5):637-650.

Jie C, Renke T, Xiaojuan G, Zhengli F, Zheng W, Zhiyan Z, Xiaoli T (2015). The functional annotation of unigenes in COG, GO, KEGG, Swissprot and Nr database.

Kennedy DO, Wightman EL (2011). Herbal extracts and phytochemicals: plant secondary metabolites and the enhancement of human brain function. Advances in Nutrition 2(1):32-50.

Khan N, Adhami VM, Mukhtar H (2010). Apoptosis by dietary agents for prevention and treatment of prostate cancer. Endocrine Related Cancer 17(1):R39-R52.

Li LL, Cheng H, Yuan HH, Xu F, Cheng SY, Cao FL (2014). Functional characterization of the Ginkgo biloba chalcone synthase gene promoter in transgenic tobacco. Genetics & Molecular Research 13(2):3446-3460.

Pang Y, Shen G, Wu W, Liu X, Lin J, Tan F, … Tang K (2005). Characterization and expression of chalcone synthase gene from Ginkgo biloba. Plant Science 168(6):1525-1531.

Qian L, Liu Y, Qi Y, Jiao S, Tian F, Jiang L, Wang Y (2014). Transcriptome sequencing and metabolite analysis reveals the role of delphinidin metabolism in flower colour in grape hyacinth. Journal of Experimental Botany 65(12):3157-3164.

Schiavon M, Dall'Acqua S, Mietto A, Pilonsmits EA, Sambo P, Masi A, Malagoli M (2013). Selenium fertilization alters the chemical composition and antioxidant constituents of tomato (Solanum lycopersicon L.). Journal of Agricultural & Food Chemistry 61:10542-10554.

Schmittgen TD, Livak KJ (2008). Analyzing real-time PCR data by the comparative C(T) method. Nature Protocols 3(6):1101.

Thavarajah D, Ruszkowski J, Vandenberg A (2008). High potential for selenium biofortification of lentils (Lens culinaris L.). Journal of Agricultural and Food Chemistry 56(22):10747-10753.

Tian XY, Wang ZY (2008). Effects of selenium application on content, distribution and accumulation of selenium, flavonoids and rutin in tartary buckwheat. Plant Nutrition & Fertilizer Science 14(4):721-727.

Winkel-Shirley B (2002). Biosynthesis of flavonoids and effects of stress. Current Opinion in Plant Biology 5(3):218-223.

Xu F, Cai R, Cheng S, Du H, Wang Y (2008). Molecular cloning, characterization and expression of phenylalanine ammonia-lyase gene from Ginkgo biloba. African Journal of Biotechnology 7(6):721-729.

Xu F, Cheng S, Cheng S, Wang Y, Du H (2007). Time course of expression of chalcone synthase gene in Ginkgo biloba. Journal of Plant Physiology and Molecular Biology 33(4):309.

Xu F, Li L, Zhang W, Cheng H, Sun N, Cheng S, Wang Y (2012). Isolation, characterization, and function analysis of a flavonol synthase gene from Ginkgo biloba. Molecular Biology Reports 39(3):2285-2296.

Xu F, Ning Y, Zhang W, Liao Y, Li L, Cheng H, Cheng S (2014a). An R2R3-MYB transcription factor as a negative regulator of the flavonoid biosynthesis pathway in Ginkgo biloba. Functional & Integrative Genomics 14(1):177-189.

Xu Q, Yin XR, Zeng JK, Ge H, Song M, Xu CJ, … Chen KS (2014b). Activator- and repressor-type MYB transcription factors are involved in chilling injury induced flesh lignification in loquat via their interactions with the phenylpropanoid pathway. Journal of Experimental Botany 65(15):4349-4359.

Ye J, Chen Q, Tao T, Xu F, Cheng S (2016). Promotive effects of genistein on photosynthesis, flavonoid biosynthesis and antioxidant enzyme activities in Ginkgo biloba. International Journal of Agriculture and Biology 18(5):917-922.

Zhai R, Wang Z, Zhang S, Meng G, Song L, Wang Z, … Xu L (2016). Two MYB transcription factors regulate flavonoid biosynthesis in pear fruit (Pyrus bretschneideri Rehd.). Journal of Experimental Botany 67(5):1275-1284.

Zhang W, Xu F, Cheng S, Liao Y (2018). Characterization and functional analysis of a MYB gene (GbMYBFL) related to flavonoid accumulation in Ginkgo biloba. Genes & Genomics 40(1):49-61.

Published
2019-06-06
How to Cite
LI, L., YU, J., YUAN, H., ZHA, S., DENG, K., XIAO, X., LUO, Y., CHENG, S., & CHENG, H. (2019). High-Density Kinetic Analysis of the Metabolomic and Transcriptomic Response of Ginkgo biloba Flavonoids Biosynthesis to Selenium Treatments. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47(3). https://doi.org/10.15835/nbha47311477
Section
Research Articles