Optimization of a Rapid Propagation System for Mass Production of High-Quality Plantlets of Trichosanthes kirilowii cv. ‘Yuelou-2’ via Organogenesis

Authors

  • Xiao Dong CAI Yangtze University, College of Horticulture and Gardening, Jingzhou, Hubei 434025 (CN)
  • Jin Jin CHENG Yangtze University, College of Horticulture and Gardening, Jingzhou, Hubei 434025 (CN)
  • Yuan Shan ZHANG Yangtze University, College of Horticulture and Gardening, Jingzhou, Hubei 434025 (CN)

DOI:

https://doi.org/10.15835/nbha47311450

Keywords:

in vitro; morphological characterization; plant growth regulator; rapid propagation; Trichosanthes kirilowii

Abstract

Trichosanthes kirilowii Maxim is a perennial plant possessing great medicinal and edible value. In this study, an efficient rapid propagation system was developed for in vitro production of high-quality plantlets of T. kirilowii cv. ‘Yuelou-2’ via organogenesis. Shoots were established from the nodal stems of this plant by cultured on Murashige and Skoog (MS) basal medium containing different concentrations of naphthalene acetic acid (NAA), indole-3-butyric acid (IBA) and 6-benzyladenine (6-BA) according to a L9 (34) orthogonal array design. MS medium supplemented with 0.5 mg l-1 6-BA alone conferred significant enhancement of shoot induction and development by variance and statistical analysis of shoot induction frequency, shoot length, as well as lateral shoot number and total node number per explant. Rooting percentage, root morphological characteristics, plantlet survival rate, as well as plantlet growth performance in vitro and ex vitro were comprehensively assessed, and results showed that the addition of 1.0 mg l-1 NAA to 1/2 MS medium was the most responsive for root induction and production of high-quality plantlets from the regenerated shoots. The in vitro propagation system described here has the potential for large-scale and rapid production of in vitro plantlets of seed variety of T. kirilowii in a sustainable manner.

References

Aremu AO, Plačková L, Pěnčík A, Novák O, Doležal K, Van Staden J (2016). Auxin-cytokinin interaction and variations in their metabolic products in the regulation of organogenesis in two Eucomis species. New Biotechnology 33(6):883-890.

Asayesh ZM, Vahdati K, Aliniaeifard S (2017). Investigation of physiological components involved in low water conservation capacity of in vitro walnut plants. Scientia Horticulturae 224:1-7.

Barrales-López A, Robledo-Paz A, Trejo C, Espitia-Rangel E, Rodríguez-De La OJL (2015). Improved in vitro rooting and acclimatization of Capsicum chinense Jacq. plantlets. In Vitro Cellular & Developmental Biology-Plant 51(3):274-283.

Colombi T, Walter A (2017). Genetic diversity under soil compaction in wheat: root number as a promising trait for early plant vigor. Frontiers in Plant Science 8:420.

Devendra NK, Rajanna L, Sheetal C, Seetharam YN (2008). In vitro clonal propagtion of Trichosanthes cucumerina L. var. cucumerina. Plant Tissue Culture and Biotechnology 18(2):103-111.

EL-Kazzaz AA, Ebad FA, EL-Sadek MEA (2018). Acclimation of potato via in vitro microtubers versus plantlets under saline conditions. Scientia Agriculturae 21(2):49-56.

Guo XL, Wang M, Shu SH (2009). Tissue culture of male and female Trichosanthes kirilowii Maxim. Journal of Wuhan Botanical Research 27(6):684-687 (in Chinese with English abstract).

Huang Y, He P, Bader KP, Radunz A, Schmid GH (2000). Seeds of Trichosanthes kirilowii, an energy-rich diet. Zeitschrift für Naturforschung 55(3-4):189-194.

Li MX, Yeung HW, Pan LP, Chan SI (1991). Trichosanthin, a potent HIV-1 inhibitor, can cleave supercoiled DNA in vitro. Nucleic Acids Research 19(22):6309-6312.

Liu GQ, Gilding EK, Godwin ID (2013). Additive effects of three auxins and copper on sorghum in vitro root induction. In Vitro Cellular & Developmental Biology-Plant 49(2):191-197.

Lo HY, Li TC, Yang TY, Li CC, Chiang JH, Hsiang CY, Ho TY (2017). Hypoglycemic effects of Trichosanthes kirilowii and its protein constituent in diabetic mice: the involvement of insulin receptor pathway. BMC Complementary and Alternative Medicine 17(1):53.

Oliveira C, Degenhardt-Goldbach J, Bettencourt GMF, Amano E, Franciscon L, Quoirin M (2017). Micropropagation of Eucalyptus grandis × E. urophylla AEC 224 clone. Journal of Forestry Research 28(1):29-39.

Padilla IMG, Webb K, Scorza R (2003). Early antibiotic selection and efficient rooting and acclimatization improve the production of transgenic plum plants (Prunus domestica L.). Plant Cell Reports 22(1):38-45.

Resende CF, Braga VF, Pereira PF, Silva CJ, Vale VF, Bianchetti RE, … Peixoto PHP (2016). Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae). Brazilian Journal of Biology 76(1):218-227.

Sarasan V, Cripps R, Ramsay MM, Atherton C, McMichen M, Prendergast G, Rowntree JK (2006). Conservation in vitro of threatened plants-progress in the past decade. In Vitro Cellular & Developmental Biology-Plant 42(3): 206-214.

Schaller GE, Bishopp A, Kieber JJ (2015). The yin-yang of hormones: cytokinin and auxin interactions in plant development. The Plant Cell 27(1):44-63.

Shaw PC, Lee MK, Wong KB (2005). Recent advances in trichosanthin, a ribosome-inactivating protein with multiple pharmacological properties. Toxicon 45(6):683-689.

Shu SH, Xie GZ, Guo XL, Wang M (2009). Purification and characterization of a novel ribosome-inactivating protein from seeds of Trichosanthes kirilowii Maxim. Protein Expression and Purification 67(2):120-125.

Wang WF, Wang LZ, Jiang JX (2009). Fatty acid profile of Trichosanthes kirilowii Maxim. seed oil. Chemical Papers 63(4):489-492.

Xu KD, Chang YX, Zhang J, Wang PL, Wu JX, Li YY, … Li CW (2015). A lower pH value benefits regeneration of Trichosanthes kirilowii by somatic embryogenesis, involving rhizoid tubers (RTBs), a novel structure. Scientific Reports 5:8823.

Yu HL, Hu YH, Li J (2009). Study on rapid propagation in vitro of medicinal Plant Trichosanthes kirilowii. Hubei Agricultural Sciences 48(2):272-274 (in Chinese with English abstract).

Zhao FL, Wang R, Xue JP, Duan, YB (2018). Efficient callus-mediated regeneration and in vitro root tuberization in Trichosanthes kirilowii Maxim, a medicinal plant. In Vitro Cellular & Developmental Biology-Plant 54(6):621-625.

Zheng Q, Sun YF, Yang AX, Xing H, Zhao H (2015). New variety of high-yielding Trichosanthes kirilowii var. Yuelou-2. Journal of Changjiang Vegetables 23:22 (in Chinese).

Zheng SS, Yuan HY, Wang LJ, An CC, Chen ZL (2001). The tissue culture of medicinal plant Trichosanthes kirilowii and its protein analysis. Chinese Journal of Biotechnology 17(4):420-422 (in Chinese with English abstract).

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Published

2019-05-19

How to Cite

CAI, X. D., CHENG, J. J., & ZHANG, Y. S. (2019). Optimization of a Rapid Propagation System for Mass Production of High-Quality Plantlets of Trichosanthes kirilowii cv. ‘Yuelou-2’ via Organogenesis. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47(3), 722–728. https://doi.org/10.15835/nbha47311450

Issue

Vol. 47 No. 3 (2019)

Section

Research Articles
CITATION
DOI: 10.15835/nbha47311450

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Copyright (c) 2019 Xiao Dong CAI, Jin Jin CHENG, Yuan Shan ZHANG

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