Physiological Effects of the Fungicide Azoxystrobin on Wheat Seedlings under Extreme Heat

Chiu-Yueh LAN; Kuan-Hung LIN; Wen-Dar HUANG; Chang-Chang CHEN

doi:10.15835/nbha47311448

Authors

Chiu-Yueh LAN National Taiwan University, Department of Agronomy, Daan, Taipei 101 (TW)
Kuan-Hung LIN Chinese Culture University, Department of Horticulture and Biotechnology, Shilin, Taipei 114 (TW)
Wen-Dar HUANG National Taiwan University, Department of Agronomy, Daan, Taipei 101 (TW)
Chang-Chang CHEN National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei (TW)

DOI:

https://doi.org/10.15835/nbha47311448

Keywords:

antioxidant activity; azoxystrobin; chlorophyll fluorescence; heat stress; wheat

Abstract

Azoxystrobin (AZ) is not only a fungicide used for disease control, but also a protective chemical for crops against specific stresses. The physiological mechanism of the fungicide AZ in protecting against heat (HT, 46 °C) stress in wheat (Triticum aestivum L.) seedlings was investigated. ‘Taichung SEL-2’ variety seedlings were pretreated with 0.4, 4, 40, 80, and 120 mg L^-1 of AZ for 4 d. Next AZ-pretreated and untreated seedlings were subjected to HT for 1 h followed by 1000 μmol m^-2s^-1 lighting for 20 min. HT induced oxidant stress which resulted in a decrease in the reducing power, an increase in malondialdehyde, and enhanced enzyme activities of ascorbate peroxidase (APX) and catalase (CAT) in leaves of untreated seedlings. However, AZ-pretreated seedlings under HT displayed reductions in chlorophyll fluorescence, APX and CAT activities, and the 1,1-diphenyl-2-picryl-hydrazyl scavenging capacity. Physiological damage caused by HT was aggravated by an increase in the AZ concentration. In addition, increased photosynthetic pigments were also observed in leaves of AZ-pretreated and HT-exposed seedlings. The results suggest that AZ does not provide a protective effect against HT stress.

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