Arbuscular Mycorrhiza Improves Leaf Food Quality of Tea Plants

  • Ya-Dong SHAO Yangtze University, College of Horticulture and Gardening, Jingzhou, Hubei 434025
  • De-Jian ZHANG Yangtze University, College of Horticulture and Gardening, Jingzhou, Hubei 434025
  • Xian-Chun HU Yangtze University, College of Horticulture and Gardening, Jingzhou, Hubei 434025
  • Qiang-Sheng WU Yangtze University, College of Horticulture and Gardening, Jingzhou, Hubei 434025
  • Chang-Jun JIANG Anhui Agricultural University, State Key Laboratory of Tea Plant Biology and Utilization, Hefei, Anhui 230036
  • Xiu-Bing GAO Tea Research Institute, Guizhou Province Academy of Agricultural Science, Guiyang, Guizhou 550006
  • Kamil KUČA University of Hradec Kralove, Faculty of Science, Department of Chemistry, Hradec Kralove 50003, Czech Republic
Keywords: soil microorganism; sucrose; symbiotic fungi; tea polyphenol; white tea

Abstract

Tea (Camellia sinensis) plants inhabit arbuscular mycorrhizal fungi (AMF) in rhizosphere, whereas it is not clear whether AMF improves leaf food quality of tea plants. A potted study was conducted to determine effects of Claroideoglomus etunicatum, Diversispora spurca, D. versiformis and a mixture of the three AMF species on leaf sugar, amino acid, soluble protein, tea polyphenol, catechuic acid, and flavonoid contents of Camellia sinensis ‘Fuding Dabaicha’ seedlings. After 12 weeks of AMF inoculation, mycorrhizal plants recorded significantly higher shoot biomass and total leaf area, whilst the effect was ranked as C. etunicatum > D. spurca > mixed-AMF > D. versiformis in the decreasing order. AMF treatments significantly increased leaf total amino acid concentrations, accompanied with up-regulation of amino acid synthetic enzymes genes glutamine synthetase (CsGS), glutamate synthase (CsGOGAT) and glutamate dehydrogenase (CsGDH). Leaf glucose, sucrose, total soluble protein, tea polyphenol, catechuic acid, and flavonoid contents were significantly higher in AMF- than in non-AMF-inoculated plants. In addition, mycorrhizal inoculation notably up-regulated the expression level of leaf 3-hydroxy-3-methylglutaryl coenzyme gene (CsHMGR), ascorbate peroxidase gene (CsAPX), and tea caffeine synthase 1 gene (CsTCS1). These results implied that AMF inoculation had positive effects on leaf food quality partly by means of up-regulation of relevant gene expression in ‘Fuding Dabaicha’ seedlings.

 

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.

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Published
2019-04-10
How to Cite
SHAO, Y.-D., ZHANG, D.-J., HU, X.-C., WU, Q.-S., JIANG, C.-J., GAO, X.-B., & KUČA, K. (2019). Arbuscular Mycorrhiza Improves Leaf Food Quality of Tea Plants. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47(3). https://doi.org/10.15835/nbha47311434
Section
Research Articles