Evaluation of the Phytochemical, Antioxidant, Antibacterial and Anticancer Activity of Prunus domestica Fruit

Hossam S. EL-BELTAGI, Abeer E. EL-ANSARY, Mai A. MOSTAFA, Teba A. KAMEL, Gehan SAFWAT

Abstract


Plants have been used long ago through man history of life for their use in food and medicinal drives. In modern life, natural products have been extracted and isolated from several kinds of plants for the development of new drugs. There are numerous interests in natural antioxidants extracted from medicinal plants, vegetables and fruits, which might help to prevent oxidative damage. One of such plants is plum Prunus domestica L., family Rosaceae. Samples from ‘African Rose’, and ‘Santa Rosa’ plum cultivars were collected from local market in Giza governorate, Egypt. The main phytochemicals of plums (fruit flesh and skin) were analyzed. Total polyphenols, flavonoids, tannins, anthocyanins, and reducing power were higher in ‘African Rose’ fruit. The ethanolic and ethyl acetate extracts of two plum cultivars were both high in the antioxidant effect with IC50 13.923 and 18.416 μg/ml of ethanolic extract of ‘African Rose’, and ‘Santa Rosa’ respectively. The IC50 of ‘African Rose’ and ‘Santa Rose’ extract against Caco-2 was 4 and 8.5 μg/ml. GC-MS analysis was carried out, fourteen and twenty one compound were identified in ‘Santa Rosa’ and ‘African Rose’ respectively. The fruits had an antimicrobial action against gram positive and negative bacteria. There was anticancer activity against 3 cell lines: Liver cell line (HepG2), colorectal adenocarcinoma (Caco-2) cell line, and breast cell line (MCF-7).


Keywords


antibacterial; anticancer activity; antioxidant; DPPH; flavonoid; phenols; plum extract

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References


Abdel-Rahim EA, El-Beltagi HS (2010). Constituents of apple, parsley and lentil edible plants and their therapy treatments for blood picture as well as liver and kidneys functions against lipidemic disease. Electronic Journal of Environmental, Agricultural and Food Chemistry 9(6):1117-1127.

Ateya AM, Abou-Hashem M, El-Sayed Z, Abbas F (2014). Biological activity of the Egyptian medicinal plants: part 4 Cytotoxicity of 50 Egyptian plants and spices against hepatocellular carcinoma. Journal of Ethnomedicine 1:56-63.

Bagavathi PE, Ramasamy N (2012). GC-MS analysis of phytocomponents in the ethanol extract of Polygonum chinense L. Pharmacognosy Research 4(1):11-14.

Bauer AW, Kirby WM, Sherris JC, Turck M (1966). Antibiotic susceptibility test-ing by a standardized single disk method. American Journal of Clinical Pathology 45(4):493-496.

Cai Y, Luo Q, Sun M, Corke H (2004). Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sciences 74(17):2157-2184.

Celep E, Aydın A, Yeşilada E (2012). A comparative study on the in vitro antioxidant potentials of three edible fruits: Cornelian cherry, Japanese persimmon and cherry laurel, Food and Chemical Toxicology 50(9):3329-3335.

Celik F, Gundogdu M, Alp S, Muradoglu F, Ercişli S,Gecer MK, Canan I (2017). Determination of phenolic compounds, antioxidant capacity and organic acids contents of Prunus domestica L., Prunus cerasifera Ehrh. and Prunus spinosa L. fruits by HPLC. Acta Chromatographica 29(4):507-510.

Chai Q, Wu B, Liu W, Wang L, Yang C, Wang Y, Fang J, Liu Y, Li S (2012). Volatiles of plums evaluated by HSSPME with GC–MS at the germplasm level. Food Chemistry 130(2):432-440.

Chou HJ, Kuo JT, Lin ES (2009). Comparative antioxidant properties of water extracts from different parts of beefsteak plant (Perilla frutescens). Journal of Food and Drug Analysis 17(6):489-496.

Coelho E, Rocha SM, Delgadillo I, Coimbra MA (2006). Headspace-SPME applied to varietal volatile compounds evolution during Vitis vinifera L. cv. ‘Baga’ ripening. Analytica Chimica Acta 563(1-2):204-214.

Costa JGM, Leite GO, Dubois AF, Seeger RL, Boligon AA, Athayde ML, Campos AR, Rocha JBT (2012). Antioxidant effect of Stryphnodendron rotundifolium Martius extracts from Cariri-Ceará state (Brazil): Potential involvement in its therapeutic use. Molecules 17(1):934-950.

Cuvelier ME, Richard H, Berset C (1992). Comparison of the antioxidative activity of some acid-phenols: Structure activity relationships. Bioscience, Biotechnology, and Biochemistry 56(2):324-325.

Dai J, Mumper RJ (2010). Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules 15(10):7313-7352.

Demir S, Turan I, Demir F, Ayazoglu Demir E, Aliyazicioglu Y (2017). Cytotoxic effect of Laurocerasus officinalis extract on human cancer cell lines. Marmara Pharmaceutical Journal 21:121-126.

Dhingra N, Sharma R, Kar A (2014). Evaluation of the antioxidant activities of Prunus domestica whole fruit: an in vitro study. International Journal of Pharmacy and Pharmaceutical Sciences 6:271-276.

Díaz-Mula HM, Zapata PJ, Guillén F, Martínez-Romero D, Castillo S, Serrano M, Valero D (2009). Changes in hydrophilic and lipophilic antioxidant activity and related bioactive compounds during postharvest storage of yellow and purple plum cultivars. Postharvest Biology and Technology 51(3):354-363.

Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocker P, Vidal N (2006). Antioxidant activity of some algerian medicinal plants extracts containing phenolic compounds. Food Chemistry 97(4):654-660.

Dugalic K, Sudar R, Viljevac M, Josipovic M, Cupic T (2014). Sorbitol and sugar composition in plum fruits influenced by climatic conditions. Journal of Agricultural Science and Technology 16(5):1145-1155.

Eivind V, Rune SS (2006). Methods to determine antioxidative capacity in fruit. Journal of Fruit and Ornamental Plant Research 14:123-131.

El-Beltagi HES (2011). Effect of roasting treatments on protein fraction profiles, some enzyme activities of Egyptian peanuts. International Journal of Food Science and Nutrition 62(5):453-456.

El-Beltagi HS, Mohamed HI, Megahed BMH, Gamal M, Safwat G (2018). Evaluation of some chemical constituents, antioxidant, antibacterial and anticancer activities of Beta vulgaris L. root. Fresenius Environmental Bulletin 27(9):6369-6378.

Erkan N (2012). Antioxidant activity and phenolic compounds of fractions from Portulaca oleracea L. Food Chemistry 133(3):775-781.

Gil MI, Tomás-Barberán FA, Hess-Pierce B, Kader AA (2002). Antioxidant capacities, phenolic compounds, carotenoids, and vitamin C contents of nectarine, peach, and plum cultivars from California. Journal of Agricultural and Food Chemistry 50(17):4976-4982.

Goupy P, Hugues M, Biovin P, Amiot MJ (1999). Antioxidant composition and activity of barley (Hordeum vulgare) and malt extracts and of isolated phenolic compounds. Journal of the Science of Food and Agriculture 79(12):1625-1634.

Harbone JB (1998). A guide to modern techniques of plant analysis. Second Ed, Chapman and Hall, London, pp 54-84.

Islam NU, Amin R, Shahid M, Amin M, Zaib S, Iqbal J (2017). A multi-target therapeutic potential of Prunus domestica gum stabilized nanoparticles exhibited prospective anticancer, antibacterial, urease-inhibition, anti-inflammatory and analgesic properties. BMC Complementary and Alternative Medicine 17(1):276.

Jeyanthi RL, Sharmila S, Das MP, Seshiah C (2014). Extraction and purification of carotenoids from vegetables. Journal of Chemical and Pharmaceutical Research 6(4):594-598.

Karabegovic IT, Stojicevic SS, Velickovic DT, Nikolic NC, Lazic ML (2014). Optimization of microwave-assisted extraction of cherry laurel fruit. Separation Science and Technology 49(3):416-423.

Kayano S, Kikuzaki H, Fukutsuka N, Mitani T, Nakatani N (2002). Antioxidant activity of prune (Prunus domestica L.) constituents and a new synergist. Journal of Agricultural and Food Chemistry 50(13):3708-3712.

Kesba HH, El-Beltagi HS (2012). Biochemical changes in grape rootstocks resulted from humic acid treatments in relation to nematode infection. Asian Pacific Journal of Tropical Biomedicine 2(4):287-293.

Kikuzaki H, Kayano SI, Fukutsuka N, Aoki A, Kasamatsu K, Yamasaki Y, Mitani T, Nakatani N (2004). Abscisic acid related compounds and lignans in prunes (Prunus domestica L.) and their oxygen radical absorbance capacity (ORAC). Journal of Agricultural and Food Chemistry 52(2):344-349.

Kim DO, Jeong SW, Lee CY (2003). Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chemistry 81(3):321-326.

Kobeasy MI, El-Beltagi HS, El-Shazly MA, Khattab EA (2011). Induction of resistance in Arachis hypogaea L. against Peanut mottle virus by nitric oxide and salicylic acid. Physiological and Molecular Plant Pathology 76(2):112-118.

Lee J, Durst RW, Wrolstad RE (2005). Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: collaborative study. Journal of AOAC International 88(5):1269-1278.

Li Y, Brown RW, Bonner MR, Deng F, Tian L, Mu L (2014). Positive relationship between total antioxidant status and chemo-kines observed in adults. Oxidative Medicine and Cellular Longevity 693680.

Lombardi-Boccia G, Lucarini M, Lanzi S, Aguzzi A, Cappelloni M (2004). Nutrients and antioxidant molecules in yellow plums (Prunus domestica L.) from conventional and organic productions: A comparative study. Journal of Agricultural and Food Chemistry 52(1):90-94.

Mattila P, Astola J, Kumpulainen J (2000). Determination of flavonoids in plant material by HPLC with diode-array and electro-array detections. Journal of Agricultural and Food Chemistry 48(12):5834-5841.

Mehta S, Soni N, Satpathy G, Gupta RK (2014). Evaluation of nutritional, phytochemical, antioxidant and antibacterial activity of dried plum (Prunus domestica). Journal of Pharmacognosy and Phytochemistry 3(2):166-171.

Orhan EI, Akkol KE (2011). Estimation of neuroprotective effects of Laurocerasus officinalis Roem. (cherry laurel) by in vitro methods. Food Research International 44(3):818-822.

Park HR, Park E, Rim AR, Jeon KI, Huang JH, Lee SC (2006). Antioxidant activity of extracts from Acanthopanax senticosus. African Journal of Biotechnology 5(23):2388-2396.

Phongpaichit S, Nikom J Rungjindamai N, Sakayaroj J, Hutadilok-Towatana N, Rukachaisirikul V, Kirtikara K (2007). Biological activities of extracts from endophytic fungi isolated from Garcinia plants. FEMS Immunology and Medical Microbiology 51(3):517-525.

Poongodi T, Srikanth R, Lalitha G (2015). Phytochemistry, GC-MS analysis and in vitro cytotoxic activity of Prunus angustifolia leaves against MCF-7 breast cancer cell line. World Journal of Pharmacy and Pharmaceutical Sciences 4:1489-1499.

Prior RL (2003). Fruits and vegetables in the prevention of cellular oxidative damage. The American Journal of Clinical Nutrition 78(3):570-578.

Repetto G, del Peso A, Zurita JL (2008). Neutral red uptake assay for the estimation of cell viability/cytotoxicity. Nature Protocols 3(7):1125-1131.

Sandra V, Ante G, Zoran S, Nadica D, Stjepan P, Jasmina D (2009). Chemical composition and antioxidant capacity of three plum cultivars. Agriculturae Conspectus Scientificus 74(3):273-276.

Saxena V, Mishra G, Saxena A, Vish-wakarma KR (2013). A comparative study on quantitative estimation of tannins in Terminalia chebula, Terminalia belerica, Terminalia arjuna and Saraca indica using spectrophotometer. Asian Journal of Pharmaceutical and Clinical Research 6(3):148-149.

Shallan MA, El-Beltagi HS, Mona AM, Amera TM, Sohir NA (2010). Effect of amylose content and pre-germinated brown rice on serum blood glucose and lipids in experimental animal. Australian Journal of Basic and Applied Sciences 4(2):114-121.

Singleton V, Rossi J (1965). Colorimetry of total phenolics with phosphomolibdic-phosphotungstic acid reagents. The American Journal of Enology and Viticulture 16(3):144-158.

Sójka M, Kołodziejczyk K, Milala J, Abadias M, Viñas I, Guyot S, Baron A (2015). Composition and properties of the polyphenolic extracts obtained from industrial plum pomaces. Journal of Functional Foods 12:168-178.

Sumathy N, Sumathy J (2011). Antibacterial and antifungal activity of musa fruit peels against skin and gastrointestinal tract diseases. Herbal Tech Industry 2:9-11.

Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J (2007). Free radicals and antioxidants in normal physiological functions and human disease. The International Journal of Biochemistry and Cell Biology 39(1):44-84.

Vinson JA, Su X, Zubik L, Bose P (2001). Phenol antioxidant quantity and quality in foods: fruits. Journal of Agricultural and Food Chemistry 49(11):5315-5321.

Vlaic R, Socaci S, Muresan AE, Muresan C, Moldovan OP, Muste S, Muresan V (2017). Bioactive compounds and volatile profile dynamics during fruit growth of several plums cultivars. Journal of Agricultural Science and Technology 19:1565-1576.

Yang J, Martinson TE, Liu RH (2009). Phytochemical profiles and antioxidant activities of wine grapes. Food Chemistry 116(1):332-339.

Yaqeen Z, Naqvi N-u-H, Sohail T, Fatima N, Imran H (2013). Screening of solvent dependent antibacterial activity of Prunus domestica. Pakistan Journal of Pharmaceutical Sciences 26(2):409-414.

Zhishen J, Mengcheng T, Jianming W (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry 64(4):555-559.




DOI: http://dx.doi.org/10.15835/nbha47111402

June 1, 2018: Notulae Botanicae Horti Agrobotanici Cluj-Napoca in Scopus ElsevierCiteScore 2017=0.78,Horticulture; Agronomy and Crop Science; Plant Science

 
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