Influence of Selected Antibiotics on the Tomato Regeneration in In Vitro Cultures
Generally, antimicrobial agents are frequently used in micropropagation techniques to obtain free elite clones or after genetic transformation to select putative transformants. Their successful application minimizes bacterial contamination however; they may be phytotoxic and may diversely affect the regeneration ability in plant tissue cultures. The objective of the current study was to estimate the effects of four antibiotics i.e. ampicillin, carbenicillin, cefotaxime and kanamycin on morphogenesis of three Polish tomato cultivars, cultured on MS medium with phytohormones. In this experiment the ability of tomato cotyledon explants to regenerate entire plants via indirect organogenesis was tested. Among four antibiotics tested, kanamycin was most harmful for the explants. This antibiotic, even at low doses (10-20 mg/L), inhibited tomato morphogenesis. On the other hand, the current study revealed significant influence of different concentrations of ampicillin, carbenicillin, cefotaxime on the frequency of bud formation. While the addition of cefotaxime at low concentration (100-200 mg/L) stimulated the bud formation, its increasing concentration adversely affected the organogenesis of tomato. The results clearly pointed out that carbenicillin and ampicillin at low concentrations (100-400 mg/L) were not only non-toxic, but they promoted bud regeneration. The obtained results show the crucial role not only of use of efficient antibiotics, but also of their proper doses in obtaining successful transformation and regeneration of tomato.
Ahmed MB, Akhter MS, Hossain M, Islam R, Chaudhary TA, … Ahmed I (2007). An efficient Agrobacterium-mediated genetic transformation method of lettuce (Lactuca sativa L.) with an aphidicidal gene pta (Pinellia ternate Agglutinin). Middle East Journal of Scientific Research 2(2):155-160.
Bosela MJ (2009). Effect of β-lactam antibiotics, auxins, and cytokinins on shoot regeneration from callus cultures of two hybrid aspens, Populus tremuloides x P. tremula and P. x canescens x P. gradientata. Plant Cell Tissue and Organ Culture 98(3):249-261.
Farzaneh A, Adel Y, Ali N, Younes G (2013). Determine effective concentration of β-lactam antibiotics against three strains of Agrobacterium tumefaciens and phytotoxity on tomato and tobacco. International Journal of Agronomy and Plant Production 11(4):2919-2925.
Gambhir G, Kumar P, Srivastava DK (2017). Effect of antibiotic sensitivity on different cultured tissues and its significance in genetic transformation of cabbage Brassica oleracea. Bioscience Biotechnology Research Communications 10(4):652-661.
Gerszberg A (2018).Tissue culture and genetic transformation of cabbage (Brassica oleracea var. capitata): an overview. Planta 248:1037-1048.
Gerszberg A, Hnatuszko-Konka K, Kowalczyk T, Kononowicz AK (2016). Efficient in vitro callus induction and plant regeneration protocol for different Polish tomato cultivars. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 44(2):452-458.
Gerszberg A, Hnatuszko-Konka K, Kowalczyk T (2015a). In vitro regeneration of eight cultivars of Brassica oleracea var. capitata In Vitro Cellular and Developmental Biology-Plant 51(1):80-87.
Gerszberg A, Hnatuszko-Konka K. Kowalczyk T, Kononowicz AK (2015b). Tomato (Solanum lycopersicum L.) in the service of biotechnology. Plant Cell Tissue and Organ Culture 120(3):881-902.
Grewal D, Gill R, Gosal SS (2006). Influence of antibiotic cefatoxime on somatic embryogenesis and plant regeneration in indica rice. Biotechnology Journal 1(10):1158-1162.
Grzebelus E, Skop L (2014). Effect of β-lactam antibiotics on plant regeneration in carrot protoplast cultures. In Vitro Cellular and Developmental Biology-Plant 50(5):568-575.
Haddadi F, Aziz MA, Abdullah SNA, Tan SG, Kamaladini H (2015). An efficient Agrobacterium-mediated transformation of strawberry cv. Camarosa by dual plasmid system. Molecules 20(3):3647-3666.
Katayama N, Takano H, Sugiyama M, Takio S, Sakai A, … Ono K (2003). Effects of antibiotics that inhibit the bacterial peptidoglycan synthesis pathway on moss chloroplast division. Plant Cell Physiology 44(7):776-781.
Kazemi EM, Jonoubi P, Majd A, Pazhouhandeh, M (2014). Reduction of negative effects of cefatoxime in tomato transformation by using FeEDDHA. International Journal of Farming and Allied Sciences 3(5):538-542.
Khokan EH, Hayder A, Ara T, Alam MK, Sharma MD (2009). Enhancement of Agrobacterium-mediated transformation method for the production of heme-protein (Ferritin protein) rich potato. International Journal of Agriculture and Crop Sciences 4(2009):17-22.
Mahadev MD, Panathila CS, Naidu CV (2014). Influence of bavistin, cefotaxime, kanamycin and silver thiosulphate on plant regeneration of Solanum viarum (Dunal)- an important anticancer medicinal plant. American Journal of Plant Science 5(3):403-408.
Mamidala P, Swamy Nanna R (2009). Influence of antibiotics on regeneration efficiency in tomato. Plant Omics 2(4):135-140.
Meng Q, Liu Z, Zhang Y, Liu C, Ren F, Feng H (2014). Effects of antibiotics on in vitro – cultured cotyledons. In Vitro Cellular and Developmental Biology-Plant 50(4):436-441.
Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15(3):473-497.
Naderi D, Askari-Khorasgani O, Mahmoudi E (2016). Cefotaxime and benzyladenine improve melon regeneration. Iranian Journal of Biotechnology 14 (1):56.
Naing AH, Park KI, Lim SH, Kim CHK (2014). Appropriate choice of antibiotics for plant regeneration and optimization of selective agents to be used in genetic transformation of chrysanthemum. Plant Omics 7(4): 237-243.
Pantahula CS, Mahadev MDN, Naidu CV (2014). The stimulatory effects of the antimicrobial agents bavistin, cefotaxime and kanamycin on in vitro plant regeneration of Centella asiatica (L.) – an important antijaundice medicinal plant. American Journal Plant Sciences 5(3):279-285.
Saporta R, De La Torre F, Segura A, Vidal JR (2014). Toxic effect of antibiotics in grapevine (Vitis vinifera 'Albariño') for embryo emergence and transgenic plant regeneration from embryogenic cell suspension. Vitis 53(2):89-94.
Shaikh S, Fatima J, Shakil S, Rizvi SMD, Kamal MA (2015). Antibiotic resistance and extended spectrum beta-lactamases: Types, epidemiology and treatment. Saudi Journal of Biological Sciences 22(1):90-101.
Sharma C, Aggarwal G, Srivastava DK (2012). Effect of antibiotic kanamycin on cultured cotyledon and hypocotyl tissues of tomato (Solanum lycopersicum cv. ‘Solan vajr’). International Journal of Agriculture Environment and Biotechnology 5(2):77-82.
Sun S, XP, Xing XJ, Xu XY, Cheng J, … Xing GM (2015). Agrobacterium-mediated transformation of tomato (Lycopersicon esculentum L. cv. ‘Hezuo908’) with improved efficiency. Biotechnology and Biotechnological Equipment 29(5): 861-868.
Tambarussi EV, Rogalski M, Nogueira FTS, Brondani GE, De Martin VF, Carrer H (2015). Influence of antibiotics on indirect organogenesis of teak. Annals of Forest Research 58(1):177-183.
Wei T, Harris L, Newton RJ (2003). Influences of antibiotics on plantlet regeneration via organogenesis in loblolly pine (Pinus taeda L.). Journal of Forestry Research 14(3):185-190.
Zhang BH, Liu F, Li ZH, Wang HM, Yao CB (2001). Effects of kanamycin on tissue culture and somatic embryogenesis in cotton. Plant Growth Regulation 33(2):137-149.
Open Access Journal:
The journal allows the author(s) to retain publishing rights without restriction. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author.