Cloning and Functional Characterization of CsUGD2 in Cucumber (Cucumis sativus L.)
UGD gene encodes UDP-glucose dehydrogenase (UGD) which is a key enzyme in the biosynthesis of cell wall, and it catalyses the irreversible oxidation of UDP-glucose (UDP-Glc) into UDP-glucuronic acid (UDP-GlcA). In cucumber, the expression level of CsUGD2 genes was higher in phloem tissues of pedicel and fruit than that in stalk. This study investigated the function of CsUGD2 in cucumber by different methods. Structure analysis indicated that CsUGD2 gene only has an exon with a length of 1,443 bp. Protein alignment suggested that UGD protein was highly conservative in different species. Phylogenetic analysis showed that CsUGD2 protein and CmoUGD2 protein form a same clade which is far away from UGDs in Arabidopsis. Real-time fluorescence quantitative analysis of CsUGD2 in different tissues of cucumber in the same period showed that CsUGD2 expressed highest in the root of cucumber. When we transformed CsUGD2 into wild type Arabidopsis, the roots of transgenic plants were shorter and the flowering time was delayed. These results suggested that CsUGD2 may play an important role in the development of cucumber roots and only act on the development of cucumber fruit when compared with other plants fruits. However, the role of CsUGD2 in regulating the growth and development process of cucumber need to further study.
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Amino SI, Takeuchi Y, Komamine A (1985). Changes in enzyme activities involved in formation and interconversion of UDP-sugars during the cell cycle in a synchronous culture of Catharanthus roseus. Plant Physiology 64(1):111-117.
Bindschedler LV, Wheatley E, Gay E, ... Bolwell GP (2005). Characterisation and expression of the pathway from UDP-glucose to UDP-xylose in differentiating tobacco tissue. Plant Molecular Biology 57(2):285-301.
Clough SJ, Bent AF (1998). Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant Journal 16(6):735-743.
Desprez T, Vernhettes S, Fagard M, ... Hofte H (2002). Resistance against herbicide isoxaben and cellulose deficiency caused by distinct mutations in same cellulose synthase isoform CESA6. Plant Physiology 128(2):482-490.
Hubber DJ (1983). Polyuronide degradation and hemicellulose modifications in ripening tomato fruit. Journal of the American Society for Horticultural Science 108:405-409.
Jarvis MC (1984). Structure and properties of pectin gels in plant cell walls. Plant Cell and Environment 7(3):153-164.
Klinghammer M, Tenhaken R (2007). Genome-wide analysis of the UDP-glucose dehydrogenase gene family in Arabidopsis, a key enzyme for matrix polysaccharides in cell walls. Journal of Experimental Botany 58(13):3609-3621.
Lamesch P, Berardini TZ, Li D, David S, Christopher W, Rajkumar S, ... Margarita GH (2012). The Arabidopsis Information Resource (TAIR): improved gene annotation and new tools. Nucleic Acids Research 40 (D1):D1202-D1210.
Oka T, Jigami Y (2006). Reconstruction of de novo pathway for synthesis of UDP-glucuronic acid and UDP-xylose from intrinsic UDP-glucose in Saccharomyces cerevisiae. FEBS Journal 273(12):2645-2657.
Paterson AH, Bowers JE, Bruggmann R, Dubchak I, Grimwood J, Gundlach H, ... Poliakov A (2009). Sorghum bicolor genome and the diversification of grasses. Nature 457(7229):551-556.
Peter R (1991). Cellulose biosynthesis and function in bacteria. Microbiology Reviews 55(1):35-58.
Robertson D, Beech I, Bolwell GP (1995). Regulation of the enzymes of UDP-sugar metabolism during differentiation of French bean. Phytochemistry 39(1):21-28.
Sakurai N (1991). Cell wall functions in growth and development-a physical and chemical point of view. Journal of Plant Research 104(1075):235-251.
Seitz B, Klos C, Wurm M, Tenhaken R (2000). Matrix polysaccharide precursors in Arabidopsis cell walls are synthesized by alternate pathways with organ-specific expression patterns. The Plant Journal 21(6):537-546.
Siddique S, Sobczak M, Tenhaken R, Grundler FMW, Bohlmannet H (2012). Cell wall ingrowths in nematode induced syncytia require UGD2 and UGD3. Plos One 7(7):e41515-e41526.
Sieberth V, Rigg GP, Roberts IS, Jann K (1995). Expression and characterization of UDPGlc dehydrogenase (KfiD), which is encoded in the type-specific region 2 of the Escherichia coli k5 capsule genes. Journal of Bacteriology 177(15):4562-4565.
Tenhaken R (1996). Cloning of an enzyme that synthesizes a key nucleotide-sugar precursor of hemicellulose biosynthesis from soybean: UDP-Glucose dehydrogenase. Plant Physiology 112(3):1127-1134.
Wen F, Zhu Y, Hawes MC (1999). Effect of pectin methylesterase gene expression on pea root development. Plant Cell 11(6):1129-1140.
Xu W, Purugganan MM, Polisensky DH, Antosiewicz DM, Fry SC, Braam J (1995). Arabidopsis TCH4, regulated by hormones and the environment, encodes a xyloglucan endotransglycosylase. Plant Cell 7(10):1555-1567.
Zhang B, Zambryski PC (2010). Divergent metabolome and proteome suggest functional independence of dual phloem transport systems in cucurbits. Proceedings of the National Academy of Sciences USA 107(30):13532-13537.
Zhao J, Li Y, Ding L, Yan S, Liu M, Jiang L, ... Liu R (2015). Phloem transcriptome signatures underpin the physiological differentiation of the pedicel, stalk and fruit of cucumber(Cucumis sativus L.). Plant and Cell Physiology 57 (1):19-34.
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