Qualitative and Quantitative Study of Quercetin and Glycyrrhizin in In Vitro Culture of Liquorice (Glycyrrhiza glabra L.) and Elicitation with AgNO3

Authors

  • Farnaz TAHOORI Islamic Azad University, Science and Research Branch, Department of Biology, Tehran, Iran
  • Ahmad MAJD Islamic Azad University, North Tehran Branch, Department of Biology, Tehran, Iran
  • Taher NEJADSATTARI Islamic Azad University, Science and Research Branch, Department of Biology, Tehran, Iran
  • Hamideh OFOGHI Iranian Research Organization for Science and Technology (IROST), Department of Biotechnology, Tehran, Iran
  • Alireza IRANBAKHSH Islamic Azad University, Science and Research Branch, Department of Biology, Tehran, Iran

DOI:

https://doi.org/10.15835/nbha47111275

Keywords:

anti-ethylene, biotechnology, elicitor, flavonoids, high performance liquid chromatography, non-biological stresses, terpenoids

Abstract

Liquorice (Glycyrrhiza glabra L.) is a plant that has been considered for a long time due to its valuable secondary metabolites. This study was conducted to obtain quercetin and glycyrrhizin under controlled conditions and the use of silver nitrate (AgNO3) as an elicitor to increase their production. For this purpose, the seeds were cultured in MS media containing various concentrations of AgNO3 (0, 2, 4, 8 and 10 mg L-1). Quercetin in the aerial parts extract of three-month seedlings prepared with methanol solvent 95% and acetic acid (9:1), and glycyrrhizin in the root extract of four-month seedlings prepared with ethanolic extract (30%) were evaluated qualitatively and quantitatively using HPLC. The results obtained from three replications showed the presence of quercetin and glycyrrhizin in all samples. The amount of quercetin in all samples treated with AgNO3 was significantly higher than control (P≤0.05) and this increase was higher at concentrations of 8 and 10 mg L-1 in comparison with other concentrations. Glycyrrhizin content increased under the influence of different concentrations of AgNO3 as compared to the control; however, this increase was not significant. Our results clearly showed that this method is a practical method to produce and elicit more these compounds with medicinal value.

References

Agati G, Azzarello E, Pollastri S, Tattini M (2012). Flavonoids as antioxidants in plants: location and functional significance. Plant Science 196:67-76.

Bais HP, Sudha GS, Ravishankar GA (2000). Putrescine and silver nitrate influences shoot multiplication, in vitro flowering and endogenous titers of polyamines in Cichorium intybus L. cv. Lucknow local. Journal of Plant Growth Regulation 19(2):238-248.

Bota C, Deliu C (2011). The effect of copper sulphate on the production of flavonoids in Digitalis lanata cell cultures. Farmacia 59(1):113-118.

Bourgaud F, Gravot A, Milesi S, Gontier E (2001). Production of plant secondary metabolites: a historical perspective. Plant Science 161(5):839-851.

Chondrogianni N, Kapeta S, Chinou I, Vassilatou K, Papassideri I, Gonos ES (2010). Anti-ageing and rejuvenating effects of quercetin. Experimental Gerontology 45(10):763-771.

Curtis RW (1981). Light requirement for AgNO3 inhibition of ethrel-induced leaf abscission from cuttings of Vigna radiata. Plant Physiology 68(6):1249-1252.

Dastgir G, Rizvi MA (2016). Glycyrrhiza glabra L. (Liquorice). Pakistan Journal of Pharmaceutical Sciences 29(5):1727-1733.

D??az J, Bernal A, Pomar F, Merino F (2001). Induction of shikimate dehydrogenase and peroxidase in pepper (Capsicum annuum L.) seedlings in response to copper stress and its relation to lignification. Plant Science 161(1):179-188.

Dmitriensko SG, Kudrinskaya VA, Apyari VV (2012). Methods of extraction, preconcentration and determination of quercetin. Journal of Analytical Chemistry 67:299-311.

Edwards RL, Lyon T, Litwin SE, Rabovsky A, Symons JD, Jalili T (2007). Quercetin reduces blood pressure in hypertensive subjects. The Journal of Nutrition 137(11):2405-2411.

Feild TS, Lee DW, Holbrook NM (2001). Why leaves turn red in autumn. The role of anthocyanins in senescing leaves of red-osier dogwood. Plant Physiology 127(2):566-574.

Fryzova R, Pohanka M, Martinkova P, Cihlarova H, Brtnicky, M, Hladky J, Kynicky J (2018). Oxidative stress and heavy metals in plants. Reviews of Environmental Contamination and Toxicology Volume 245:129-156.

Fuente CDL, Ortega-Ortiz H, Benavides-Mendoza A, Sandoval-Rangel A (2014). Effect of the application of silver nitrate on antioxidant status in watermelon plants. Pakistan Journal of Botany 46(5):1843-1846.

Fukai T, Cai B-s, Maruno K, Miyakawa Y, Konishi M, Nomura T (1998). An isoprenylated flavanone from Glycyrrhiza glabra and rec-assay of licorice phenols. Phytochemistry 49(7):2005-2013.

Groppa MD, Tomaro ML, Benavides MP (2007). Polyamines and heavy metal stress: the antioxidant behavior of spermine in cadmium-and copper-treated wheat leaves. Biometals 20(2):185-195.

Harwansh R, Patra K, Pareta S, Singh J, Biswas R (2011). Pharmacological studies on Glycyrrhiza glabra: a review. Pharmacologyonline 2(1):1032-1038.

Jaimand K, Ahrabi Asli H, Behrad Z (2013). Extraction and determination of quercetin and kampferol in Foeniculum vulgare Mill. Iranian Journal of Medicinal and Aromatic plants 29:681-691.

Kanwal H, Sherazi BA (2017). Herbal medicine: Trend of practice, perspective, and limitations in Pakistan. Asian Pacific Journal of Health Sciences 4(4):6-8.

Karaogul E, Parlar P, Parlar H, Alma MH (2016). Enrichment of the glycyrrhizic acid from licorice roots (Glycyrrhiza glabra L.) by isoelectric focused adsorptive bubble chromatography. Journal of Analytical Methods in Chemistry 7201740.

Khalaf I, Vlase L, Lazar D, Corciova A, Ivanescu B, Lazar MI (2010). HPLC-UV-MS study of polyphenols from Glycyrrhiza glabra. Farmacia 58(4):416-421.

Kubalt K (2016). The role of phenolic compounds in plant resistance. Biotechnology and Food Science 80(2):97-108.

Kumar S, Pandey AK (2013). Chemistry and biological activities of flavonoids: an overview. The Scientific World Journal 162750.

Kumar V, Parvatam G, Ravishankar GA (2009). AgNO3: A potential regulator of ethylene activity and plant growth modulator. Electronic Journal of Biotechnology 12(2):8-9.

Larson AJ, Symons JD, Jalili T (2010). Quercetin: a treatment for hypertension? – A review of efficacy and mechanisms. Pharmaceuticals 3(1):237-250.

Liao J, Qu B, Zheng N (2016). Extraction of glycyrrhizic acid from Glycyrrhiza uralensis using ultrasound and its process extraction model. Applied Sciences 6(11):319.

Maalik A, Khan FA, Mumtaz A, Mehmood A, Azhar S, Atif M, Tariq I (2014). Pharmacological applications of quercetin and its derivatives: a short review. Tropical Journal of Pharmaceutical Research 13(9):1561-1566.

Mierziak J, Kostyn K, Kulma A (2014). Flavonoids as important molecules of plant interactions with the environment. Molecules 19(10):16240-16265.

Mlcek J, Jurikova T, Skrovankova S, Sochor J (2016). Quercetin and its anti-allergic immune response. Molecules 21(5):623.

Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15(3): 473-497.

Nagaich U, Gulati N, Chauhan S (2016). Antioxidant and Antibacterial Potential of Silver Nanoparticles: Biogenic Synthesis Utilizing Apple Extract. Journal of Pharmaceutics 7141523.

Naik P, Al-Khayri J (2016). Impact of abiotic elicitors on in vitro production of plant secondary metabolites: a review. Journal of Advanced Research in Biotechnology 1(2):1-7.

Nammi S, Boini MK, Lodagala SD, Behara RBS (2003). The juice of fresh leaves of Catharanthus roseus Linn. reduces blood glucose in normal and alloxan diabetic rabbits. BMC Complementary and Alternative Medicine 3(1):4.

Panche AN, Diwan AD, Chandra SR (2016). Flavonoids: an overview. Journal of Nutritional Science 5, e47.

Patel H, Krishnamurthy R (2013). Elicitors in plant tissue culture. Journal of Pharmacognosy and Phytochemistry 2(2):60-65.

Perry J, Shin D, Getzoff E, Tainer J (2010). The structural biochemistry of the superoxide dismutases. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics 1804(2):245-262.

Racchi ML (2013). Antioxidant defenses in plants with attention to Prunus and Citrus spp. Antioxidants 2(4):340-369.

Raja R, Sreenivasulu M (2015). Medicinal plants secondary metabolites used in pharmaceutical importance – An overview. World Journal of Pharmacy and Pharmaceutical Sciences 4(4):436-447.

Ramirez-Estrada K, Vidal-Limon H, Hidalgo D, Moyano E, Golenioswki M, Cusidó RM, Palazon J (2016). Elicitation, an effective strategy for the biotechnological production of bioactive high-added value compounds in plant cell factories. Molecules 21(2):182.

Ranganathan V, Punniamurthy N (2013). Estimation of phenol contents in Glycirrhiza glabra by thin layer chromatography and spectrophotometry. International Journal of Agricultural Sciences and Veterinary Medicine 1(3):24-27.

Rates SMK (2001). Plants as source of drugs. Toxicon 39(5):603-613.

Schäfer H, Wink M (2009). Medicinally important secondary metabolites in recombinant microorganisms or plants: progress in alkaloid biosynthesis. Biotechnology Journal 4(12):1684-1703.

Seca AM, Pinto DC (2018). Plant secondary metabolites as anticancer agents: successes in clinical trials and therapeutic application. International Journal of Molecular Sciences 19(1):263.

Sewelam N, Kazan K, Schenk PM (2016). Global plant stress signaling: reactive oxygen species at the cross-road. Frontiers in Plant Science 7:187.

Shabani L, Ehsanpour AA, Asghari G, Emami J (2009). Glycyrrhizin production by in vitro cultured Glycyrrhiza glabra elicited by methyl jasmonate and salicylic acid. Russian Journal of Plant Physiology 56(5):621-626.

Shakeran Z, Keyhanfar M, Asghari G, Ghanadian M (2015). Improvement of atropine production by different biotic and abiotic elicitors in hairy root cultures of Datura metel. Turkish Journal of Biology 39(1):111-118.

Sharma P, Dubey RS (2005). Lead toxicity in plants. Brazilian Journal of Plant Physiology 17(1):35-52.

Sharma P, Jha AB, Dubey RS, Pessarakli M (2012). Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of Botany 217037.

Singh B, Mungara P, Nivsarkar M, Anandjiwala S (2009). HPTLC densitometric quantification of glycyrrhizin, glycyrrhetinic acid, apigenin, kaempferol and quercetin from Glycyrrhiza glabra. Chromatographia 70(11-12):1665.

Smetanska I (2008). Production of secondary metabolites using plant cell cultures. Food Biotechnology 111:187-228.

Sytar O, Kumar A, Latowski D, Kuczynska P, Strzalka K, Prasad M (2013). Heavy metal-induced oxidative damage, defense reactions, and detoxification mechanisms in plants. Acta Physiologiae Plantarum 35(4):985-999.

Tamimi SM (2015). Effects of ethylene inhibitors, silver nitrate (AgNO3), cobalt chloride (CoCl2) and aminooxyacetic acid (AOA), on in vitro shoot induction and rooting of banana (Musa acuminata L.). African Journal of Biotechnology 14:2511-2516.

Tholl D (2015). Biosynthesis and biological functions of terpenoids in plants. In: Schrader J, Bohlmann J (Eds). Biotechnology of isoprenoids. Advances in Biochemical Engineering/Biotechnology. Springer, Cham 148:63-106.

Tian M, Yan H, Row KH (2008). Extraction of glycyrrhizic acid and glabridin from licorice. International Journal of Molecular Sciences 9(4):571-577.

Tiwari R, Rana C (2015). Plant secondary metabolites: a review. International Journal of Engineering Research and General Science 3:661-670.

Treutter D (2006). Significance of flavonoids in plant resistance: a review. Environmental Chemistry Letters 4(3):147.

Tripathy BC, Oelmüller R (2012). Reactive oxygen species generation and signaling in plants. Plant Signaling & Behavior 7(12):1621-1633.

Tumova L, Polivkova D (2006). Effect of AgNO3 on the production of flavonoids by the culture of Ononis arvensis L. in vitro. Ceska Slov Farm 55(4):186-188.

Turhan H (2004). The effect of silver nitrate (ethylene inhibitor) on in vitro shoot development in potato (Solanum tuberosum L.). Biotechnology 3(1):72-74.

Vildova A, Hendrychova H, Kubes J, Tumova L (2016). Influence of AgNO3 Treatment on the Flavonolignan production in cell suspension culture of Silybum marianum (L.) Gaertn. World Academy of Science, Engineering and Technology, International Journal of Biological, Biomolecular, Agricultural, Food and Biotechnological Engineering 8(8):959-962.

Wink M (2010). Function and biotechnology of plant secondary metabolites. Annual Plant Reviews 39.

Wong SP, Leong LP, Koh JHW (2006). Antioxidant activities of aqueous extracts of selected plants. Food Chemistry 99(4):775-783.

Yang L, Wen KS, Ruan X, Zhao YX, Wei F, Wang Q (2018). Response of plant secondary metabolites to environmental factors. Molecules 23(4):762.

Zeka K, Ruparelia K, Arroo RR, Budriesi R, Micucci M (2017). Flavonoids and their metabolites: prevention in cardiovascular diseases and diabetes. Diseases 5(3):19.

Zhang Y (2004). Cancer-preventive isothiocyanates: measurement of human exposure and mechanism of action. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 555(1):173-190.

Zhao J, Fujita K, Sakai K (2005). Oxidative stress in plant cell culture: a role in production of ??thujaplicin by Cupresssus lusitanica suspension culture. Biotechnology and Bioengineering 90(5):621-631.

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Published

2018-10-16

How to Cite

TAHOORI, F., MAJD, A., NEJADSATTARI, T., OFOGHI, H., & IRANBAKHSH, A. (2018). Qualitative and Quantitative Study of Quercetin and Glycyrrhizin in In Vitro Culture of Liquorice (Glycyrrhiza glabra L.) and Elicitation with AgNO3. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47(1), 143–151. https://doi.org/10.15835/nbha47111275

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Research Articles
CITATION
DOI: 10.15835/nbha47111275

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