Determination of the Effect of Humic Acid on Growth and Development Parameters of Parsley (Petroselinum sativum Hoffm.) Grown in Boron Soil



Boron is an important micronutrient, required for all plant growth, and critical for high yield and quality of crops. The aim of the present research was to determine the effects of boron on pot-grown parsley (Petroselinum sativum Hoffm.). The experimental design consisted of four treatments using Hoagland-Arnon (1950) nutrient solutions with two different boron concentrations (B1 - 15 ppm and B2 - 150 ppm), each with and without 10 ml humic acid addition (HB1 and HB2), and controls with full strength Hoagland-Arnon solutions. Growth analyses of the parsley revealed that 15 ppm boron application caused an increase in root length leaf fresh and dry weight root fresh and dry weight and leaf area compared to control values. 150 ppm B (B2) concentration decreased all growth parameters compared to controls. The two humic acid treatments (HB1 and HB2) did not increase any of those growth parameters either in controls (C) or in the two boron (B1 and B2) concentrations. Analysis by (ICP-MS) revealed that B content in the leaves increased gradually in B1 and B2, as well as in both humic treatments where in HB2 it increased to 99.38% compared to B1. In the leaves, Mn, Zn and Fe contents behaved the same as B, increasing in all treatments, with the amounts in HB2 being significantly greater than in C, B1 and B2 leaves.


boron; growth; humic acid; parsley; Petroselinum sativum; nutrient uptake

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Abdel-Mawgoud ASA, Gameh MA, Abdel-Aziz SH, El-Sayed MM (2007). Wheat water relations at various irrigation regimes with modern irrigation systems under climatic condition of Assiut governorate, Upper Egypt. Journal of Agricultural Science 32(7):6051- 6066.

Adani F, Genevini P, Zaccheo P, Zocchi G (1998). The effect of commercial humic acid on tomato plant growth and mineral nutrition. Journal of Plant Nutrition 21:561-575.

Adiloglu A, Adiloglu S (2006). The effect of boron (B) application on the growth and nutrient content of maize in zinc deficient soils. Bulgarian Journal of Agricultural Science 12:387-392.

Ahmed N, Abid M, Ahmad F (2008). Boron toxicity in irrigated cotton (Gossypium hirsutum L.). Pakistan Journal of Botany 40:2443-2445.

Akinci S, Buyukkeskin T, Eroglu AE, Erdogan BE (2009). The effect of humic acid on nutrient composition in broad bean (Vicia faba L.) roots. Notulae Scientia Biologicae 1(1):1-8.

Akinci S (2011). Humik Asitler, Bitki Büyümesi ve Besleyici Alımı [Humic Acids Plant Growth and Nutrient Uptake]. Marmara Üniversitesi Fen Bilimleri Dergisi 23(1):46-56 (In Turkish).

Arancon NQ, Lee S, Edwards A, Atiyeh R (2003). Effects of humic acids derived from cattle, food and paper-waste vermicomposts on growth of greenhouse plants. Pedobiologia 47:741-744.

Aref F (2011). Concentration of zinc and boron in corn leaf as affected by zinc sulfate and boric acid fertilisers in a deficient soil. Life Science Journal 8:26-31.

Atiyeh RM, Lee S, Edwards CA, Arancon NQ, Metzger JD (2002). The influence of humic acids derived from earthworm-processed organic wastes on plant growth. Bioresource Technology 84(1):7-14.

Bayrak H, Onder M, Gezgin S (2005). Bor uygulamasınının nohut (Cicer arietinum L. ) cesitlerinde verim ve bazi verim unsurlarına etkileri [Effects on yield and some yield components of boron application in chickpea (Cicer arietinum L.) varieties]. Selçuk Üniversitesi Ziraat Fakültesi Dergisi 19(35):66-74 (In Turkish).

Bidegain RA, Kaemmerer M, Guiresse M, Hafidi M, Rey F, Morard P, Revel JC (2000). Effects of humic substances from composted or chemically decomposed poplar sawdust on mineral nutrition of ryegrass. Journal of Agricultural Sciences 134:259-267.

Brown PH, Hu H (1996). Phloem mobility of boron is species dependent. Evidence for phloem mobility in sorbitol rich species. Annals of Botany 77:497-505.

Brown PH, Bellaloui N, Wimmer MA, Bassil ES, Ruiz J, Hu H, Pfeffer H, Dannel F, Romheld VV (2002). Boron in plant biology. Plant Biology 4:205-223.

Brown PH, Shelp BJ (1997). Boron mobility in plants. Plant and Soil 193: 85-101.

BUGEM (2017). BUGEM General Directorate of Plant Production. Turkish agricultural statistics for 2017. Republic of Turkey Ministry of Agriculture and Forestry Ankara. Retrieved 2017 April 13 from (In Turkish).

Carter MR, Gregorich EG (2008). Soil Sampling and Methods of Analysis (2nd ed). CRC Press, Boca Raton.

Cartwright B, Tiller KG, Zarcinas BA, Spouncer LR (1983). The chemical assessment of the boron status of soils. Australian Journal of Soil Research 2:321-332.

Cikili Y, Samet H, Dursun S (2013). Effects of potassium treatment on alleviation of boron toxicity in cucumber plant (Cucumis sativus L). Soil-Water Journal 2:719-726.

Cimrin KM, Karaca S, Bozkurt MA (2001). Mısır bitkisinin gelisimi ve beslenmesi üzerine hümik asit ve NPK uygulamalarının etkisi [The effect of NPK and humic acid applications on growth and nutrition of corn plant (Zea mays L.)]. Tarim Bilimleri Dergisi 7(2):95-100 (In Turkish).

Dannel F, Pfeffer H, Romheld V (2000). Characterization of root boron pools, boron uptake and boron translocation in sunflower using the stable isotope 10B and 11B. Australian Journal of Plant Physiology 156:756-761.

Dannel F, Pfeffer H, Romheld V (2002). Update on boron in higher plant-Uptake, primary translocation and compartmentation. Plant Biology 4:193-204.

David PP, Nelson PV, Sanders DC (1994). A humic acid improves growth of tomato seedling in solution culture. Journal of Plant Nutrition 17:173-184.

Dursun A, Guvenc I, Turan M (1999). Macro and micro nutrient contents of seedlings andtheir effects on seedling growth in relation to humic acid application. In: Anac D, Martin-Prevel P (Eds). Improved Crop Quality by Nutrient Management. Kluwer Academic Publishers, Dordrecht, Boston, London.

Eaton FM (1944). Deficiency, toxicity, and accumulation of boron in plants. Journal of Agricultural Research 69: 237-277.

Eyheraguibel B, Silvestre J, Morard P (2008). Effects of humic substances derived from organic waste enhancement on the growth and mineral nutrition of maize. Bioresource Technology 99:4206-4212.

Fagbenro JA, Agboola AA (1993). Effect of different levels of humic acid on the growth and nutrient uptake of teak seedlings. Journal of Plant Nutrition 16:1465-1483.

Fageria NK, Baligar VC, Jones CA (2011). Growth and mineral nutrition of field crops, 3rd edition. CRC Press, Boca Raton.

Ferrara G, Pacifigo A, Simeone P, Ferrara E (2007). Preliminary study on the effects of foliar applications of humic acids on Italia table grape. XXXth Worl Congress of Vine and Wine, Budapest, Hungary (Vol 165).

Gezgin S, Hamurcu M (2006). The importance of the nutrient elements interaction and the interactions between boron with the other nutrients elements in plant nutrition. Selçuk University, Yearbook of the Faculty of Agriculture, pp 24-31 (In Turkish).

Goldberg S (1997). Reactions of boron with soils. Plant and Soil 193:35-48.

Gunes A, Alpaslan M, Cikili Y, Ozcan H (2000). The effect of zinc on alleviation of boron toxicity in tomato plants (Lycopersicon esculentum L.). Turkish Journal of Agriculture and Forestry 24:505-509.

Gupta UC (1983). Boron deficiency and toxicity symptoms for several crops as related to tissue boron levels. Journal of Plant Nutrition 6:387-395.

Gupta UC (1993). Boron and its role in crop production. CRC Press: Boca Raton, FL.

Gupta UC, Jame YW, Campbell CA, Leyshon AJ, Nocholaivhuk W (1985). Boron toxicity and deficiency: A review. Canadian Journal of Soil Science 65(3):381-409.

Gupta UC (2006). Chapter 8. Boron. In: Hand book of plant nutrition. Barker AV, Pilbeam DJ (Eds) CRC Press, Boca Raton, FL, USA pp 241-277.

Hamurcu M, Gezgin S (2007). Bor ve çinko uygulamasının bazı bodur fasulye (Phaseolus vulgaris L.) genotiplerinin biyolojik verim değerlerine etkisi [Effect of boron and zinc applications on biomass values of dwarf bean (Phaseolus vulgaris L.) genotypes]. Selçuk Üniversitesi Ziraat Fakültesi Dergisi 21(41):11-22 (In Turkish).

Herrera-Rodríguez MB, González-Fontes A, Rexach J, Camacho-Cristóbal JJ, Maldonado JM, Navarro-Gochicoa MT (2010). Role of boron in vascular plants and response mechanisms to boron stresses. Plant Stress 4:115-122.

Hoagland DR, Arnon DI (1950). The water-culture method for growing plants without soil. California Agricultural Experimental Station Circular 347 (Univ California, Berkeley).

Hoffman GL, Nikols DJ, Stuhec S, Wilson RA (1993). Evaluation of leonardite (humalite) resources of Alberta. Open File Report 1993–18 [Online]. Alberta Research Council. Available:

Hosseini SM, Maftoun M, Karimian N, Rounaghi A, Emam Y (2007). Effect of zinc × boron interaction on plant growth and tissue nutrient concentration of corn. Journal of Plant Nutrition 30:773-781.

Hu H, Brown P (1997). Absorption of boron by plant roots. Plant Soil 193:49-58.

Jame YW, Nicholaichuk W, Leyshon AJ, Campbell CA (1982). Boron concentration in the soil solution under irrigation: A theoretical analysis. Canadian Journal of Soil Science 62:461-470.

Kabata-Pendias A, Pendias H (1999). Biogeochemistry of trace elements, 2nd ed. Wyd. Nauk PWN, Warsaw.

Kabata-Pendias A, Pendias H (2001). Trace elements in soils and plants, 3rd Edn. CRC Press, Boca Raton, Florida, USA.

Kacar B (1972). Chemical analyses of soil and plant. Press of Ankara University Agricultural Faculty 53 (In Turkish).

Kacar B, Katkat AV, Ozturk S (2006). Bitki Fizyolojisi [Plant Physiology]. 2. Baski Nobel Yayin Dagitim. Yayin No: 848 (In Turkish).

Karaman MR, Turan M, Yıldırım E, Gunes A, Esringu A, Demirtaş A, Gürsoy A, Dizman M, Tutar A, Kilinc H (2012). Ca ve B-Humat bileşiklerinin domates (Lycopersicon esculentum L.) bitkisinin verim parametreleri ile klorofil ve stoma gecirgenligi üzerine etkilerinin belirlenmesi [Determination of effects calcium and boron humate on tomato (Lycopersicon esculentum L.) yield parameters, chlorophyll and stomatal conductivity]. Sakarya Üniversitesi Fen-Edebiyat Dergisi 14(1):177-185 (In Turkish).

Kolsarici O, Kaya MD, Day S, Ipek A, Uranbey S (2005). Effects of humic acid rate on emergence and seedling growth of sunflower (Helianthus annuus L.). Ziraat Fakultesi Dergisi, Akdeniz Universitesi (2):151-155 (In Turkish).

Kord M, Derakhshan L, Memarian H, Tajabadipour A (2010). Effects of high boron concentration on boron uptake and growth of pistachio seedlings. 19th World Congress of Soil Science, Soil Solutions for a Changing World 1-6 August 2010, Brisbane, Australia pp 150-153.

Kulikova NA, Stepanova EV, Koroleva OV (2005). Mitigating activity of humic substances: Direct influence on biota. In: Use of humic substances to remediate polluted environments: From theory to practice. NATO Science Series IV: Erath and Environmental Series, Perminova IV (Eds). Kluwer Academic Publishers, USA, pp 285- 309.

Lacey A, Davies S (2009). Boron toxicity in WA soils. WA Department of Agriculture and Food Farmnote, 388/2009. Government of Western Australia.

Loomis WD, Durst RW (1991). Boron and cell walls. In: Randall DD, Blevins DG, Miles CD (Eds). Current topics in plant biochemistry and physiology, Vol 10. University of Missouri, Columbia, pp 149-178.

Mackowiak C, Grossl P, Bugbee B (2001). Beneficial effects of humic acid on micronutrient availability to wheat. Soil Science Society of America Journal 65:1744-1750.

Malik KA, Azam F (1985). Effects of humic acids on wheat (Triticum aestivum L.) seedling growth. Environmental and Experimental Botany 25:245-252.

Marschner H (1995). Mineral Nutrition of Higher Plants, 2nd. Academic Press, London.

Mead R, Curnow RN (1983). Statistical methods in agricultural and experimental biology. Chapman and Hall, London.

Mohamed H (2012). Effects of humic acid and calcium forms on dry weight and nutrient uptake of maize plant under saline condition. Australian Journal of Basic and Applied Sciences 6(8):597-604.

Nable RO, Banuelos GS, Paull JG (1997). Boron toxicity. Plant and Soil 193:181-198.

Nable RO, Cartwright B, Lance RC (1990). Genotypic differences in boron accumulation in barley: Relative susceptibilities to boron deficiency and toxicity. In: El Bassam N, Dambroth M, Loughman B (Eds). Genetic Aspects of Plant Mineral Nutrition. Kluwer Academic Publishers, Dordrecht, The Netherlands pp 243-251.

Papadakis IE, Dimassi KN, Bosabalidis AM, Therios IN, Patakas A, Giannakoula A (2004). Boron toxicity in ‘Clementine’ mandarin plants grafted on two rootstocks. Plant Science 166:539-547.

Parvaiz A, Prasad M (2012). Abiotic stress responses in plants. Springer Science+Business Media. Library of Congress Control Number: 2011940823.

Pettit RE (2004). Organic matter, humus, humate, humic acid, fulvic acid and humin: their importance in soil fertility and plant health. Retrieved 2017 January 20 from

Power PP, Woods WG (1997). The chemistry of boron and its speciation in plants. Plant Soil 193:1-13.

Rajaie M, Ejraie AK, Owliaie HR, Tavakoli AR (2009). Effect of zinc and boron interaction on growth and mineral composition of lemon seedlings in a calcareous soil. International Journal of Plant Production 3(1):39-49.

Reid R (2010). Can we really increase yields by making crop plants tolerant to boron toxicity? Plant Science 178:9-11.

Reid RJ, Hayes JE, Post A, Stangoulis JCR, Graham RD (2004). A critical analysis of the causes of boron toxicity in plants. Plant Cell and Environment 25:1405-1414.

Savasturk O (2008). Effects of soil- and foliar-applied humic acids and phosphorus on the growth and mineral nutrient content of eggplant. MSc thesis, Ankara University, Graduate School of Natural and Applied Sciences, Ankara, Turkey.

Sharif M, Khattak RA, Sarir MS (2002). Effect of different levels of lignitic coal derived humic acid on growth of maize plants. Communications in Soil Science and Plant Analysis 33(19-20):3567-3580.

Shorrocks V (1997). The occurrence and correction of boron deficiency. Plant and Soil 193:121-148.

Sinha P, Jain R, Chatterjee C (2000). Interaction effect of boron and zinc on growth and metabolism of mustard. Communications in Soil Science and Plant Analysis 3:41-49.

Stangoulis JC, Reid RJ, Brown PH, Graham RD (2001). Kinetic analysis of boron transport in Chara. Planta 213:142-146.

Stevenson FJ (1994). Humus chemistry: Genesis, composition, reactions. 2nd. Edition, John Wiley and Sons, Inc, New York. 285.

Szabolcs I (1989). Salt affected soils. CRC Press, Boca Raton, Florida, USA.

Tan KH, Nopamornbodi V (1979). Effects of different levels of humic acids on nutrient content and growth of corn (Zea mays L.). Plant and Soil 51:283-287.

Tanaka M, Fujiwara T (2007). Physiological roles and transport mechanisms of boron: perspectives from plants. Pflügers Archiv-European Journal of Physiology 456:671-677.

Tipping E (2002). Cation binding by humic substances. Cambridge University Press, Cambridge, U.K. No 12.

van Goor BJ, van Lune P (1980). Redistribution of potassium, boron, magnesium and calcium in apple trees determined by an indirect method. Physiologia Plantarum 48:21-26.

Wilcox LV (1960). Boron injury to plants, Agriculture information bulletin no. 211 Agricultural Research Service. United States Department of Agriculture, Washington, D.C.

Yau SK, Saxena MC (1997). Variation in growth development and yield of durum wheat in response to high soil boron I: Average effects. Australian Journal of Agricultural Research 48:945-949.


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