Architectural Diversity of the Cuticle and Epidermis of Native and Hybrid Tomato Fruit Genotypes and the Relation to Polygalacturonase Expression

  • Cristián VELA-HINOJOSA Universidad Autónoma Metropolitana Iztapalapa, Biotechnology PhD program, Av. San Rafael Atlixco 186, Col. Vicentina, CP 09310. México D.F.
  • Claudia BARBOSA-MARTÍNEZ Universidad Autónoma Metropolitana Iztapalapa, Departamento de Biología. Av. San Rafael Atlixco 186, Col. Vicentina, CP 09310. México D.F.
  • Héctor B. ESCALONA-BUENDÍA Universidad Autónoma Metropolitana Iztapalapa, Departamento de Biotecnología. Av. San Rafael Atlixco 186, Col. Vicentina, CP 09310. México D.F.
  • José Alberto MENDOZA-ESPINOZA Universidad Autónoma de la Ciudad de México, Colegio de Ciencias y Humanidades, Calzada Ermita Iztapalapa 4163. CP 09620. México D.F.
  • Ricardo LOBATO-ORTÍZ Colegio de Postgraduados, Departamento de Recursos Genéticos y Productividad, Campus Montecillo Km 36.5 Carretera México-Texcoco. CP 56230. Estado de México
  • Juan Enrique RODRÍGUEZ-PÉREZ Universidad Autónoma Chapingo, Departamento de Fitotecnia, Km. 38.5 Carretera México-Texcoco. CP 56230 Chapingo. Estado de México
  • Juan Manuel VILLA-HERNÁNDEZ Universidad Autónoma Metropolitana Iztapalapa, Departamento de Ciencias de la Salud, Av. San Rafael Atlixco 186, Col. Vicentina, CP 09310. México D.F.
  • Laura J. PÉREZ-FLORES Universidad Autónoma Metropolitana Iztapalapa, Departamento de Ciencias de la Salud, Av. San Rafael Atlixco 186, Col. Vicentina, CP 09310. México D.F.

Abstract

The tomato (Solanum lycopersicum L.) fruit has been widely studied because of its high consumption, nutritional value, and well-characterized genome. It also represents a good model for studying the structure and function of the cuticle, a protective film that is deposited on the surface of outer epidermal cell walls, and affects the integrity and firmness of the fruit, and, therefore, its commercialization. To evaluate the differences in their structure and function, we characterized the morphology of the cuticle and the epidermis and their relationship to firmness, brightness, weight loss, and transcript levels of polygalacturonase (PGA) in four hybrid genotypes and four native genotypes of different origins, shapes, and colors. Our results show a different architecture of the epidermis and cell wall among the genotypes. Native genotypes showed irregular-shaped epidermal cells and a thicker epidermis. Anticlinal pegs were also present to only one-third of the depth of the epidermal cells. The hybrid fruit surface was smoother compared to native genotypes, and it was associated with a higher level of brightness and a less weight loss. A negative correlation between firmness and PGA transcript levels was found. The chartreuse yellow genotype was the firmest, and it had the lowest levels of PGA expression. Meanwhile, the black genotype displayed the lowest weight loss and stem scar diameter. Our results suggest that several morphological features of the cuticle and epidermis have been modified through selection of the tomato fruit, altering quality parameters, such as weight loss and firmness.

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Published
2018-01-01
How to Cite
VELA-HINOJOSA, C., BARBOSA-MARTÍNEZ, C., ESCALONA-BUENDÍA, H. B., MENDOZA-ESPINOZA, J. A., LOBATO-ORTÍZ, R., RODRÍGUEZ-PÉREZ, J. E., VILLA-HERNÁNDEZ, J. M., & PÉREZ-FLORES, L. J. (2018). Architectural Diversity of the Cuticle and Epidermis of Native and Hybrid Tomato Fruit Genotypes and the Relation to Polygalacturonase Expression. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 46(1), 45-51. https://doi.org/10.15835/nbha46111001
Section
Research Articles