Cloning and Expression Analysis of a Squalene Epoxidase Gene from Ginkgo biloba

  • Li ZHU Yangtze University, College of Horticulture and Gardening, 434025, Jingzhou
  • Liang-Qiong MA Yangtze University, College of Horticulture and Gardening, 434025, Jingzhou
  • Feng XU Yangtze University, College of Horticulture and Gardening, 434025, Jingzhou
  • Xiao-Juan YAN Yangtze University, College of Horticulture and Gardening, 434025, Jingzhou
  • Wei-Wei ZHANG Yangtze University, College of Horticulture and Gardening, 434025, Jingzhou

Abstract

Squalene epoxidase is a key enzyme involved in triterpene saponins biosynthetic pathways in plants. In this study, the SE gene was isolated from Ginkgo biloba by RT-PCR, which designated as GbSE, GenBank accession number: KY751713. The length of GbSE gene is 1646 bp, contains an open reading frame of 1617 bp encoding 538 amino acids. The theoretical molecular weight and pI of GbSE protein was 58.3 kDa and 8.35, respectively. Sequence multiple alignment found that GbSE protein had high homology with SE proteins from other plants, including several highly conservative motifs and amino acids. Phylogenetic tree analysis showed that Ginkgo biloba SE belonged to the gymnospermous group, and was closely related to the SE protein of Picea sitchensis. A study of gene expression analysis indicated that the GbSE gene was highly expressed in stems, low expressed in fruits. The cDNA sequence of GbSE gene was cloned from G. biloba and the expression level of this gene was analyzed. This work can provide some theoretical basis for the research on the molecular mechanism of triterpenoid saponin biosynthesis.

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Author Biography

Wei-Wei ZHANG, Yangtze University, College of Horticulture and Gardening, 434025, Jingzhou

Published
2018-01-01
How to Cite
ZHU, L., MA, L.-Q., XU, F., YAN, X.-J., & ZHANG, W.-W. (2018). Cloning and Expression Analysis of a Squalene Epoxidase Gene from Ginkgo biloba. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 46(1), 39-44. https://doi.org/10.15835/nbha46110868
Section
Research Articles