Novel Zinc Finger-Homeodomain Gene from Barley (HvZFHD1) is Differentially Regulated During Spike Development and under Hormonal Treatments and Abiotic Stresses

Authors

  • Saeid ABU-ROMMAN Al-Balqa' Applied University, Faculty of Agricultural Technology, Department of Biotechnology, Al-Salt 19117 (JO)
  • Khaldoun AL-HADID The University of Jordan, Faculty of Science, Department of Biological Sciences, Amman 11942 (JO)

DOI:

https://doi.org/10.15835/nbha45110612

Abstract

Plant zinc finger-homeodomains (ZFHDs) are transcriptional factors that play an important role in regulating plant growth and development. Several ZFHD genes were cloned and characterized in many plant species. In the present study, a full-length cDNA sequence of ZFHD gene was cloned from barley (termed as HvZFHD1) using reverse transcription polymerase chain reaction (RT-PCR). The sequence analysis showed that the HvZFHD1 was 1477 bp in length, and contained a complete open reading frame (ORF) of 1161 bp. The deduced protein is composed of 386 amino acids, with a predicted molecular weight of 40.46 kDa and a theoretical isoelectric point of 8.5. Multiple sequence alignment indicated that HvZFHD1 protein shared high identity with ZFHD proteins from wheat, maize, and rice. The predicted HvZFHD1 protein contained the characteristic putative zinc finger domain in the N-terminus and a DNA binding homeodomain in the C-terminus. The expression level of HvZFHD1 was investigated using qRT-PCR during spike development and in response to exogenous phytohormones and abiotic stresses. The results showed that the expression level of HvZFHD1 was fluctuated during spike development with higher expression during anthesis, medium milk, late milk, and early dough stages. The expression of barley ZFHD1 was strongly responsive to abscisic acid treatment and was up-regulated in seedlings treated with methyl jasmonate, salicylic acid, and ethephone. In addition, the expression levels of HvZFHD1 were increased by dehydration, salinity, and heat stress, but not affected by cold stress. The expression patterns of HvZFHD1 suggest that it might play a role in flowering and flower development and is involved in plant responses to abiotic stresses.

Downloads

Published

2017-06-10

How to Cite

ABU-ROMMAN, S., & AL-HADID, K. (2017). Novel Zinc Finger-Homeodomain Gene from Barley (HvZFHD1) is Differentially Regulated During Spike Development and under Hormonal Treatments and Abiotic Stresses. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 45(1), 89–96. https://doi.org/10.15835/nbha45110612

Issue

Section

Research Articles
CITATION
DOI: 10.15835/nbha45110612