Evaluation of Genetic Diversity by DNA Barcoding of Local Tomato Populations from North-Western Romania

  • Marin CĂPRAR Biological Research Center, Botanical Garden “Vasile Fati” Jibou, 16 Wesselenyi Miklos Str., 455200 Jibou
  • Cristina M. COPACI Biological Research Center, Botanical Garden “Vasile Fati” Jibou, 16 Wesselenyi Miklos Str., 455200 Jibou
  • Diana M. CHENDE Biological Research Center, Botanical Garden “Vasile Fati” Jibou, 16 Wesselenyi Miklos Str., 455200 Jibou
  • Oana SICORA Biological Research Center, Botanical Garden “Vasile Fati” Jibou, 16 Wesselenyi Miklos Str., 455200 Jibou
  • Radu ȘUMĂLAN Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael Ist of Romania” from Timisoara, Faculty of Horticulture and Forestry, 119 Aradului Str., 300645 Timisoara
  • Cosmin SICORA Biological Research Center, Botanical Garden “Vasile Fati” Jibou, 16 Wesselenyi Miklos Str., 455200 Jibou

Abstract

Tomato is one of the most important crops worldwide. DNA barcoding is a molecular based method that has been successfully used for species identification, but a few studies have used this method for cultivated varieties identification. The aim of this study was to test the utility of DNA barcoding for the identification of five local salt tolerant tomato varieties and two commercial varieties. To assess the genetic diversity of tomato varieties, the non-coding plastid trnH-psbA intergenic spacer and three plastid regions (rbcL, rpoC1, rpoB) were used. Based on the sequence variation of the trnH-psbA barcode, three haplotypes were detected among the seven tomato varieties. A neighbor-joining tree was generated and separated the local tomato varieties from the commercial varieties into two distinct clusters. We found very low levels of variation in the chosen plastial markers, but additional markers could be tested in order to assess the utility of DNA barcodes in tomato varieties identification.

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Published
2017-06-10
How to Cite
CĂPRAR, M., COPACI, C. M., CHENDE, D. M., SICORA, O., ȘUMĂLAN, R., & SICORA, C. (2017). Evaluation of Genetic Diversity by DNA Barcoding of Local Tomato Populations from North-Western Romania. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 45(1), 276-279. https://doi.org/10.15835/nbha45110601
Section
Research Articles