Cryopreservation of Coffea arabica L. Zygotic Embryos by Vitrification

  • Rodrigo Therezan de FREITAS Universidade Federal de Lavras (UFLA), Departamento de Biologia, Setor de Fisiologia Vegetal, Campus Universitário, 372000-000, Lavras MG
  • Renato PAIVA Universidade Federal de Lavras (UFLA), Departamento de Biologia, Setor de Fisiologia Vegetal, Campus Universitário, 372000-000, Lavras MG
  • Thais Silva SALES Universidade Federal de Lavras (UFLA), Departamento de Biologia, Setor de Fisiologia Vegetal, Campus Universitário, 372000-000, Lavras MG
  • Diogo Pedrosa Corrêa da SILVA Universidade Federal de Lavras (UFLA), Departamento de Biologia, Setor de Fisiologia Vegetal, Campus Universitário, 372000-000, Lavras MG
  • Michele Valquíria dos REIS Universidade Federal de Lavras (UFLA), Departamento de Biologia, Setor de Fisiologia Vegetal, Campus Universitário, 372000-000, Lavras MG
  • Ana Cristina de SOUZA Universidade Federal de Lavras (UFLA), Departamento de Biologia, Setor de Fisiologia Vegetal, Campus Universitário, 372000-000, Lavras MG
  • Sttela Dellyzete Veiga Franco da ROSA EMBRAPA, Avenida W3 Norte (Final), Parque Estação Biológica, 70770-901 Brasília DF

Abstract

As a consequence of the difficulty in conventional coffee seed storage, biotechnological alternatives such as cryopreservation have been investigated. The objective of this study was to develop a protocol for the cryopreservation of Coffea arabica L. (cv. ‘Catuaí Vermelho’ - IAC 144) zygotic embryos by vitrification. For the cryopreservation study, the embryos were immersed in Plant Vitrification Solution 2 at different times (0, 10, 25, 50, 100, and 250 min) and two temperatures (0 and 25 °C). Subsequently, the best thawing time was determined in a water bath (1, 3, 5 minutes or directly in Recovery Solution). An anatomical study was conducted on non-stored and stored embryos, with or without the use of Plant Vitrification Solution 2. The immersion in cryoprotectant solution for 100 min at 0 °C allows embryo cryopreservation. Embryos can be directly thawed in Recovery Solution after storage in liquid nitrogen. It was observed that Plant Vitrification Solution 2 reduced internal water content in the cells, allowing subsequent embryo growth resumption.

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Published
2016-12-14
How to Cite
FREITAS, R. T. de, PAIVA, R., SALES, T. S., SILVA, D. P. C. da, REIS, M. V. dos, SOUZA, A. C. de, & ROSA, S. D. V. F. da. (2016). Cryopreservation of Coffea arabica L. Zygotic Embryos by Vitrification. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 44(2), 445-451. https://doi.org/10.15835/nbha44210553
Section
Research Articles