Efficient In Vitro Callus Induction and Plant Regeneration Protocol for Different Polish Tomato Cultivars

  • Aneta GERSZBERG University of Lodz, Department of Genetics, Plant Molecular Biology and Biotechnology, Banacha Street 12/16, 90-237 Lodz
  • Katarzyna HNATUSZKO-KONKA University of Lodz, Department of Genetics, Plant Molecular Biology and Biotechnology, Banacha Street 12/16, 90-237 Lodz
  • Tomasz KOWALCZYK University of Lodz, Department of Genetics, Plant Molecular Biology and Biotechnology, Banacha Street 12/16, 90-237 Lodz
  • Andrzej K. KONONOWICZ University of Lodz, Department of Genetics, Plant Molecular Biology and Biotechnology, Banacha Street 12/16, 90-237 Lodz

Abstract

The major goal of this research was to establish a stable regeneration system for tomato cultivars in order to lay the foundations for the future genetic transformation of the tomato. The regeneration ability of two kinds of explants (cotyledons and hypocotyl segments) was compared for three Polish cultivars of the tomato (Solanum lycopersicum). Explants were cultured on 10 different regeneration media (basal mediums MS or B5, and with a combination of 6-benzylaminopurine (BAP) and indole-3-acetic acid (IAA). It was found that the ability to regenerate was substantially dependent on the cultivars, as well as on the kind of explant. The best explants for inducing shoot regeneration were cotyledons, followed by hypocotyls. It was noticed that the best formulation of the medium for this regeneration from the two types of explants used in this study, is MS with 2 mg/L BA and 0.1 mg/L IAA.  Tomato shoots were transferred to a ½ MS medium and ½ MS complemented with 0.1 mg/L IAA for rooting and all of them responded positively to the rooting medium.

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Published
2016-12-14
How to Cite
GERSZBERG, A., HNATUSZKO-KONKA, K., KOWALCZYK, T., & KONONOWICZ, A. K. (2016). Efficient In Vitro Callus Induction and Plant Regeneration Protocol for Different Polish Tomato Cultivars. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 44(2), 452-458. https://doi.org/10.15835/nbha44210530
Section
Research Articles