Genetic Diversity of Salvia Species Assessed by ISSR and RAPD Markers

  • Masoumeh YOUSEFIAZARKHANIAN PhD student
  • Ali ASGHARI Associate Professor
  • Jafar AHMADI Professor
  • Behvar ASGHARI Assistant Professor
  • Ali Ashraf JAFARI Professor

Abstract

The genus Salvia includes an enormous assemblage of nearly 1,000 species dispersed around the world. Due to possible threats to this genus, there is an immediate requirement to evaluate the diversity of its wild populations. ISSR and RAPD molecular techniques were used to evaluate the genetic relationships among twenty-one ecotypes of eight Salvia species. Amplification of genomic DNA using 23 primers (15 RAPD and eight ISSR) produced 280 bands, of which 91% were polymorphic. The results of marker parameters showed no clear difference between two marker systems. It was generally observed that both ISSR and RAPD markers had similar efficiency in detecting genetic polymorphisms with remarkable ability to differentiate the closely related ecotypes of Salvia. Nei’s similarity coefficients for these techniques ranged from 0.48 to 0.98. Based on the results of clustering, PCoA and AMOVA, the genetic diversity between and within species was confirmed. So, conservation and domestication of the genus Salvia must be due to levels of genetic variations.

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Author Biographies

Masoumeh YOUSEFIAZARKHANIAN, PhD student
University of Mohaghegh Ardabili, Faculty of Agricultural and Natural Resources, Daneshgah St. Ardabil, Iran.
Ali ASGHARI, Associate Professor
University of Mohaghegh Ardabili, Faculty of Agricultural and Natural Resources, Daneshgah St. Ardabil, Iran.
Jafar AHMADI, Professor
Imam Khomeini International University, Faculty of Agriculture and Natural Resources, Qazvin, Iran
Behvar ASGHARI, Assistant Professor
Imam Khomeini International University, Faculty of Agriculture and Natural Resources, Qazvin, Iran
Ali Ashraf JAFARI, Professor
Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO), Tehran
Published
2016-12-14
How to Cite
YOUSEFIAZARKHANIAN, M., ASGHARI, A., AHMADI, J., ASGHARI, B., & JAFARI, A. A. (2016). Genetic Diversity of Salvia Species Assessed by ISSR and RAPD Markers. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 44(2), 431-436. https://doi.org/10.15835/nbha44210391
Section
Research Articles