Effect of Curcumin on Oxidative Stress in a Model of Turpentine Induced Acute Experimental Inflammation

  • Andrei CONEAC “Iuliu Hațieganu” University of Medicine and Pharmacy, Histology Department, 8 Victor Babeș Street, 400012 Cluj-Napoca
  • Meda Sandra ORASAN “Iuliu Hațieganu” University of Medicine and Pharmacy, Physiopathology Department, 8 Victor Babeș Street, 400012 Cluj-Napoca
  • Daniel Corneliu LEUCUTA “Iuliu Hațieganu” University of Medicine and Pharmacy, Department of Medical Informatics and Biostatistics, 8 Victor Babeș Street, 400012 Cluj-Napoca
  • Nicoleta DECEA “Iuliu Hațieganu” University of Medicine and Pharmacy, Physiology Department, 8 Victor Babeș Street, 400012 Cluj-Napoca
  • Miuta FILIP “Iuliu Hațieganu” University, Raluca Ripan Institute for Research in Chemistry, 30 Fântânele Street, 400294 Cluj-Napoca
  • Carmen Mihaela MIHU “Iuliu Hațieganu” University of Medicine and Pharmacy, Histology Department, 8 Victor Babeș Street, 400012 Cluj-Napoca
  • Adriana MURESAN “Iuliu Hațieganu” University of Medicine and Pharmacy, Physiology Department, 8 Victor Babeș Street, 400012 Cluj-Napoca
  • Remus Ioan ORASAN “Iuliu Hațieganu” University of Medicine and Pharmacy, Physiology Department, 8 Victor Babeș Street, 400012 Cluj-Napoca
  • Marioara MOLDOVAN “Iuliu Hațieganu” University, Raluca Ripan Institute for Research in Chemistry, 30 Fântânele Street, 400294 Cluj-Napoca

Abstract

Curcumin, a natural phenolic compound is an anti-tumor agent with anti-inflammatory and anti-oxidant properties. The aim of this research was to evaluate oxidative stress levels, the antioxidant activity and Curcumin concentrations by high performance liquid chromatography (HPLC) in an acute experimental inflammation induced by Turpentine oil (intramuscular 0.6 mg kg-1 body weight) and to compare a prophylactic versus a therapeutic regimen of Curcumin (oral suspension of 150 mg Curcumin kg-1 rat weight). Sixteen adult male Wistar rats were assigned to four groups: Control, Group I (Curcumin only), Group II (Curcumin administration, then induced inflammation after 1 hour) and Group III (induced inflammation then Curcumin administration after 2 hours). Oxidative stress was assessed by measuring serum malondialdehide and carbonylated proteins, while systemic and local total antioxidant capacity was determined by ABTS. Local tissue changes (muscle, kidney, liver) were analysed using histopathology. Results showed that acute inflammation significantly increased lipid peroxidation in Groups II and III compared to Control and Group I. A significantly reduced total antioxidant capacity (ATBS) was present in serum and kidney in Group II, also in muscle and kidney in Group III. ABTS levels were significantly increased only in the liver tissue of the animals in Groups II and III with induced inflammation as compared to Group I. This study proved the potential of Curcumin in reducing oxidative stress in both prophylactic and therapeutic regimens.

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Published
2017-06-10
How to Cite
CONEAC, A., ORASAN, M. S., LEUCUTA, D. C., DECEA, N., FILIP, M., MIHU, C. M., MURESAN, A., ORASAN, R. I., & MOLDOVAN, M. (2017). Effect of Curcumin on Oxidative Stress in a Model of Turpentine Induced Acute Experimental Inflammation. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 45(1), 65-74. https://doi.org/10.15835/nbha45110361
Section
Research Articles