Preliminary Phytochemical Analysis and Antioxidant, Antibacterial Activities of <i>Crocus alatavicus</i> from Kazakhstan

  • Dariya SATYBALDIYEVA Al-Farabi Kazakh National University, Faculty of Biology and Biotechnology, Al-Farabi 71/6, Almaty 050040
  • Valentina MURSALIYEVA Institute of Plant Biology and Biotechnology, Laboratory of Clonal Propagation, Timiryazev 45, Almaty 050040
  • Izbassar RAKHIMBAYEV Institute of Plant Biology and Biotechnology, Laboratory of Clonal Propagation, Timiryazev 45, Almaty 050040
  • Bolatkhan ZAYADAN Al-Farabi Kazakh National University, Faculty of Biology and Biotechnology, Al-Farabi 71/6, Almaty 050040
  • Ramazan MAMMADOV Pamukkale University, Faculty of Science and Letters, Kinikli 20017, Denizli, Turkey

Abstract

Phytochemical analysis of C. alatavicus revealed the presence of phenols, flavonoids, anthocyanins, carotenoids, amino acids and carbohydrates. The flavonoid, amino acids and carotenoid contents were higher in aerial part (1.50%, 7.49% and 9.78mg%, respectively) than in bulb (0.43%, 3.88% and 0.91 mg%, respectively). Total phenolic content (TPC), total antioxidant (TAA), 2.2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and antibacterial activities of water, methanol, ethanol and dichloromethane extracts from aerial part and bulb were tested. TPC ranged from 13.63 to 72.29 mg gallic acid equivalents (GAE)/g extract. The maximum TAA were observed in ethanol (61.34%) and methanol extracts (46.13%) from aerial part with a high TPC (72.29 and 62.37 mgGAE/g extract, respectively). Ethanol extracts from aerial part and bulb had good scavenger of DPPH radicals (65.5% and 54.08%, respectively) with an IC50 387 and 447 µg/ml. Ethanol extract from aerial part was most effective against gram-positive bacterial strains S. aureus, B. subtilis and B. cereus. Biological activities of the extracts were correlated with the TPC. It can be deduced that ethanol and methnol extracts of C. alatavicus contains useful potent bioactive compounds with antioxidant and antimicrobial activities.

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Published
2015-12-10
How to Cite
SATYBALDIYEVA, D., MURSALIYEVA, V., RAKHIMBAYEV, I., ZAYADAN, B., & MAMMADOV, R. (2015). Preliminary Phytochemical Analysis and Antioxidant, Antibacterial Activities of <i>Crocus alatavicus</i&gt; from Kazakhstan. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 43(2), 343-348. https://doi.org/10.15835/nbha43210089
Section
Research Articles