Modulation of the UV-B-induced Oxidative Stress and Apoptosis in HaCaT Cell Line with Calluna vulgaris Extract

  • Piroska VIRAG The Oncology Institute “Prof. Dr. Ion Chiricuta”, 34-36 Republicii, 400015 Cluj-Napoca
  • Ioana C. BRIE The Oncology Institute “Prof. Dr. Ion Chiricuta”, 34-36 Republicii, 400015 Cluj-Napoca
  • Claudia C. BURZ The Oncology Institute “Prof. Dr. Ion Chiricuta”, 34-36 Republicii, 400015 Cluj-Napoca
  • Corina B. TATOMIR The Oncology Institute “Prof. Dr. Ion Chiricuta”, 34-36 Republicii, 400015 Cluj-Napoca
  • Tudor HODOR “Iuliu Hatieganu” University of Medicine and Pharmacy, 13 Emil Isac, 400023 Cluj-Napoca
  • Remus ORĂȘAN “Iuliu Hatieganu” University of Medicine and Pharmacy, 13 Emil Isac, 400023 Cluj-Napoca
  • Hana Petra DECEAN “Iuliu Hatieganu” University of Medicine and Pharmacy, 13 Emil Isac, 400023 Cluj-Napoca
Keywords: antioxidant capacity; apoptosis; Bax-α, Bcl-xL; common heather; ROS

Abstract

The reactive oxygen species (ROS) production due to ultraviolet B (UV-B) exposure is extremely harmful to the skin. It causes lesions of DNA, proteins and lipids and leads to cellular death. In the present study the UV-B-induced ROS and subsequent apoptosis in the human keratinocyte cell line (HaCaT) were counterbalanced by a plant extract with antioxidant capacity. Some molecules modulated by common heather (Calluna vulgaris) (CV) extract through which this may exert its photoprotective effects were also identified. The ROS were evaluated with CM-H2DCFDA assay, while apoptosis and Bax-α/Bcl-xL molecules with ELISA. The extract was standardized according to its polyphenolic content and the most important biologically active compounds, such as hyperozid, quercetin, isoquercetin, kampferol were evidenced by high-performance liquid chromatography. The UV-B induced ROS production occurred at its highest level at 2 h after the exposure of the HaCaT cells, while apoptosis later, at 4 h. The most significant changes in Bax-α and Bcl-XL proteins induced by UV-B, as well as the highest effect of the extract on apoptosis, were both registered at 4 h. The CV extract decreased concentration- and time-dependently the UV-B-induced ROS production and prevented apoptosis. These effects of CV occurred, at least to a certain extent, due to the modulation of Bax-α/Bcl-XL proteins. These findings suggest that skin cells could be protected from some of the UV-B-induced harmful effects by the administration of the CV extract, which may be further exploited as a potential photoprotective agent.

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Published
2015-12-10
How to Cite
VIRAG, P., BRIE, I. C., BURZ, C. C., TATOMIR, C. B., HODOR, T., ORĂȘAN, R., & DECEAN, H. P. (2015). Modulation of the UV-B-induced Oxidative Stress and Apoptosis in HaCaT Cell Line with Calluna vulgaris Extract. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 43(2), 313-319. https://doi.org/10.15835/nbha43210000
Section
Research Articles