In vitro Antifungal Activity of a New Bioproduct Obtained from Grape Seed Proanthocyanidins on Botrytis cinerea Mycelium and Spores

Ancu?a NECHITA, Razvan V. FILIMON, Roxana M. FILIMON, Lucia-Cintia COLIBABA, Daniela GHERGHEL, Doina DAMIAN, Rodica PA?A, Valeriu V. COTEA


Botrytis cinerea is a necrotrophic fungus that affects over 200 plant species. In vineyards, this pathogen is responsible for one of the most important diseases, commonly known as botrytis bunch rot or grey mould. Keeping infection under control with synthetic fungicides leads to an increased biological resistance of pathogen populations. An alternative way to synthetic products is to obtain natural fungicides by using bioactive compounds of plants. This study focuses on the antifungal properties of a new bioproduct obtained from polymeric proanthocyanidins extracted from grape seeds of Feteasc? neagr? variety. The bioproduct in solid state presented a total content of polyphenols of 0.625 mg GAE mg-1, a polyphenolic index of 17.40 and an antioxidant activity of 91.27% scavenged DPPH. The bioproduct with polyphenolic structure showed a moderate effect on the radial growth of fungal mycelium, at EC50 values between 11.23 and 12.15 mg mL-1. Effective antifungal activity was showed in the inhibition of spore germination, where the EC50 values varied from 1.14 to 1.47 mg mL-1. These in vitro results sustain the possibility of including the bioproduct in the category of natural fungicides for biological control against Botrytis cinerea fungus.


biological activity; minimum fungicidal concentration; natural fungicide; necrotrophic fungus; polyphenolic compounds

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June 1, 2018: Notulae Botanicae Horti Agrobotanici Cluj-Napoca in Scopus ElsevierCiteScore 2017=0.78,Horticulture; Agronomy and Crop Science; Plant Science