Callus Induction in Baru (Dipteryx alata Vog.) Explants

Authors

  • Rodrigo Kelson S. REZENDE Federal University of Grande Dourados, Faculty of Agrarian Sciences, Highway Dourados - Itahum, Km 12 - University City, mailbox 364 - Zip Code 79804-970, Dourados, MS (BR)
  • Ana Maria N. SCOTON Federal University of Grande Dourados, Faculty of Agrarian Sciences, Highway Dourados - Itahum, Km 12 - University City, mailbox 364 - Zip Code 79804-970, Dourados, MS (BR)
  • Maílson V. JESUS Federal University of Grande Dourados, Faculty of Agrarian Sciences, Highway Dourados - Itahum, Km 12 - University City, mailbox 364 - Zip Code 79804-970, Dourados, MS (BR)
  • Zeva V. PEREIRA Federal University of Grande Dourados, Faculty of Biological and Environmental Sciences, Highway Dourados - Itahum, Km 12 - University City, mailbox 364 - Zip Code 79804-970, Dourados, MS (BR)
  • Fernanda PINTO Federal University of Grande Dourados, Faculty of Biological and Environmental Sciences, Highway Dourados - Itahum, Km 12 - University City, mailbox 364 - Zip Code 79804-970, Dourados, MS (BR)

DOI:

https://doi.org/10.15835/nbha47111366

Keywords:

Cerrado; Dipteryx alata Vog.; micropropagation; plant growth regulators

Abstract

Baru (Dipteryx alata Vog.) is a species with great economic and environmental potential; it has popular acceptance, besides being a very productive species. Alternative propagation methods are important for species maintenance and exploration. Thus, micropropagation emerged as an alternative technique, providing genetic stability and the production of a large number of seedlings. The aim of the present investigation was to develop a callus induction protocol for in vitro baru explants. The tested explants were nodal, internodal and foliar segments. The explants were disinfected for 30 seconds in 70% alcohol (v/v) and 2 minutes in sodium hypochlorite (1.25% active chlorine). This was followed by triple washing. The inoculation was carried out in test tubes containing 15 mL MS medium (30 g L-1 sucrose, 6 g L-1 agar and 100 mg L-1 ascorbic acid) supplemented with 2.0 mg L-1 naphthalene acetic acid (NAA). The solution also contained 0.0, 2.5 or 5.0 mg L-1 of 6-benzylaminopurine (BAP) with the pH adjusted to 5.8. In the incubation phase, the explants were cultured for seven days in the dark and then subjected to a photoperiod of 16 hours (43 µmol m-2 s-1) at 25 ± 2 °C. The treatments were studied with 2.5, 5.0, 7.5 or 10.0 mg L-1 BAP additions to the MS. Callus formation, contamination and oxidation evaluations were undertaken. The results obtained when using 2.0 mg L-1 NAA concluded that such a treatment should be used to induce callogenesis from nodal explants, while for the tested baru leaf explants, the best results for callus formation were given by the combination of 2.0 mg L-1 NAA with 2.5 mg L-1 of BAP to.

References

Arakaki AH, Scheidt GN, Portella AC, Arruda EJ, Costa RB (2009). O baru (Dipteryx alata Vog.) como alternativa de sustentabilidade em área de fragmento florestal do Cerrado, no Mato Grosso do Sul [The baru (Dipteryx alata Vog.) as a sustainability alternative in a forest fragment area of the Cerrado, in Mato Grosso do Sul]. Interações (Campo Grande) 10(1):31-39.

Araruna EC, Oliveira JPR, Pereira VJ, Asmar SA, Melo B (2017). Salt concentrations in culture media for the development of Dipteryx alata in vitro. Pesquisa Agropecuaria Brasileira 52(12):1295-1300.

Bray E, Bailey-Serres J, Weretilnyk E (2000). Responses to abiotic stresses. In: Buchanan B, Gruissem W, Jones R (Eds). Biochemistry and molecular biology of plants. American Society of Plant Physiologists, Maryland pp 1158-1203.

Buechel S, Leibfried A, To JP, Zhao Z, Andersen SU, Kieber JJ, Lohmann JU (2010). Role of A-type Arabidopsis response regulators in meristem maintenance and regeneration. European Journal of Cell Biology 89(2-3):279-284.

Candil RFM, Arruda EJ, Arakaki AH (2007). O Cumbaru (Dipteryx alata Vog.), o desenvolvimento local e a sustentabilidade biológica no assentamento Andalucia, Nioaque/MS [The Cumbaru (Dipteryx alata Vog.), local development and the biological sustainability in the Andalucia Settling, Nioaque/MS]. Interações (Campo Grande) 8(1):75-80.

Chaibub AA, Sena APA, Mesquita FL, Maeshima FHS, Oliveira SA, Faria PR, Sibov ST, Araújo LG (2016). Fungos endofíticos associados às amêndoas do baru (Dipteryx alata Vog.) durante a germinação e multiplicação in vitro [Endophytic fungi associated with baru (Dipteryx alata Vog.) almonds during in vitro germination and multiplication]. Conpeex. Retrieved 2016 June 07 from http: //www. ufg.br/conpeex/2009.

Cortleven A, Nitschke S, Klaumünzer M, Abdelgawad H, Asard H, Grimm B, Riefler M, Schmülling T (2014). A novel protective function for cytokinin in the light stress response is mediated by the AHK2 and AHK3 receptors. Plant Physiology 164:1470-1483.

Dutra LF, Wendling I, Brondani GE (2009). A micropropagação de eucalipto [The eucalyptus micropropagation]. Pesquisa Florestal Brasileira 58:49-59.

Eldessoky DS, Ismail RM, Hadi-Abdel A, Abdallah NA (2014). Establishment of regeneration and transformation system of sugarcane cultivar GT54-9 (C9). GM Crops 2(2):126-134.

Ferreira DF (2011). SISVAR (Sistema para análise de variância [Variance Analysis System]). Federal University of Lavras (Sciences Exact Departament-DEX). (CD-ROM).

Fetzer DL, Cruz PN, Hamerski F, Corazza ML (2018). Extraction of baru (Dipteryx alata vogel) seed oil using compressed solvents technology. The Journal of Supercritical Fluids 137:23-33.

Grattapaglia D, Machado MA (1998). Micropropagação [Micropro-pagation]. In: Torres AC, Caldas LS, Buso JA (Eds). Cultura de tecidos e transformação genética de plantas [Tissue culture and genetic transformation of plants]. Embrapa-SPI/Embrapa-CNPH, Brasília pp 183-260.

Larson LCRS (2014). Estratégias de propagação de barueiro (Dipteryx alata Vog.) e jatobazeiro do cerrado (Hymenaea stigonocarpa MART.) [Baru propagation strategies (Dipteryx alata Vog.) and jatobas of the Cerrado (Hymenaea stigonocarpa MART.). PhD Thesis, Paulista State University.

Lemos MRB, Siqueira EMA, Arruda SF, Zambiazi RC (2012). The effect of roasting on the phenolic compounds and antioxidant potential of baru nuts [Dipteryx alata Vog.]. Food Research International 48(2):592-597.

Lloyd G, McCown B (1980). Commercially feasible micropropagation of montaim laurel, Kalmia latifolia, by use of shoot tip culture. Combined Proceedings 30:421-427.

Lorenzi H (1998). Árvores brasileiras: manual de identificação e cultivo de plantas arbóreas nativas do Brasil [Brazilian trees: identification and cultivation of native tree plants in Brazil]. Plantarum, São Paulo.

Mendonça RC, Felfili JM, Walter BMT, Silva MG Jr, Rezende AV, Filgueiras TS, Nogueira PE, Fagg CW (2008). Flora vascular do bioma Cerrado: checklist com 12.356 espécies [Flora vascular do Bioma Cerrado: Checklist with 12,356 species]. In: Sano SM, Almeida SP, Ribeiro JF (Eds). Cerrado: ecologia e flora [Cerrado: ecology and flora]. Embrapa Cerrados, Planaltina pp 421-1279.

Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. ?Physiologia Plantarum 15(3):473-497.

Oliveira KS, Freire FAM, Aloufa MAI (2016). Efeito de 6-benzilaminopurina e ácido naftalenoacético sobre a propagação in vitro de Hancornia speciosa Gomes [6-benzylaminopurine and naphthaleneacetic acid effect on the in vitro propagation of Hancornia speciosa Gomes]. Floresta 46(3):335-342.

Pasqual M, Ramos JD, Hoffman A, Carvalho GR (1998). Reguladores de crescimento [Growth regulators]. In: Meios de cultura [Culture mediums]. UFLA/FAEPE, Lavras, cap. 3 pp 37-84.

Pott A, Pott VJ (2003). Plantas nativas potenciais para sistemas agroflorestais em Mato Grosso do Sul [Potential native plants for agroforestry systems in Mato Grosso do Sul]. In: Seminário sistemas agroflorestais e desenvolvimento sustentável [Seminar on agroforestry systems and sustainable development], Embrapa Gado de Corte, Campo Grande, CD-ROM. Retrieved 2018 March 07 from http://saf.cnpgc.embrapa.br/publicacoes/03.pdf.

Schuch MW, Erig AC (2005). Micropropagação e plantas frutíferas [Micropropagation and fruit plants]. In: Fachinello JC, Hoffmann A, Nachtigal JC (Eds). Propagação de plantas frutíferas [Fruits plants propagation]. Embrapa, Brasília pp 155-173.

Silva HFJ, Asmar SA, Oliveira RC, Luz JMQ, Melo B (2016). Alternative supplements and MS medium concentrations in the in vitro establishment of Dipteryx alata VOG. Bioscience Journal 32(5):1138-1146.

Silva LC (2012). Germinação, estabelecimento e multiplicação in vitro de Eugenia dysenterica DC. e Dipteryx alata Vogel, espécies frutíferas do Cerrado [Germination, establishment and in vitro multiplication of Eugenia dysenterica DC. and Dipteryx alata Vogel, fruit species of the Cerrado]. MSc Dissertation, Federal University of Goiás.

Soares FP, Paiva R, Alvarenga AA, Nogueira RC, Emrich EB, Martinotto C (2007). Organogênese direta em explantes caulinares de mangabeira (Hancornia speciosa Gomes) [Direct organogenesis in mangabeira stem explants (Hancornia speciosa Gomes). Revista Brasileira de Biociencias 5(S2):723-725.

Su YH, Liu YB, Zhang XS (2011). Auxin-cytokinin interaction regulates meristem development. Molecular Plant 4(4):616-625.

Taiz L, Zeiger E (2013). Fisiologia vegetal [Plant Physiology]. Artmed, Porto Alegre.

Torres AC, Caldas LS, Buso JÁ (1998). Cultura de tecidos e transformação genética de plantas [Tissue culture and genetic transformation of plants]. EMBRAPA/CBAB, Brasília.

Vieira RF, Agostini-Costa TS, Silva DB, Sano SM, Ferreira FR (2016). Espécies alimentícias nativas da região Centro-Oeste [Native species of the Central-West region]. In: Vieira RF, Camillo J, Coradin L (Eds). Espécies nativas da flora brasileira de valor econômico atual ou potencial: Plantas para o futuro: Região Centro-Oeste [Native species of Brazilian flora of current or potential economic value: Plants for the future: Central-West Region]. MMA, Brasília pp 107-351.

Werner T, Schmuulling T (2009). Cytokinin action in plant development. Current Opinion in Plant Biology 12(5):527-538.

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Published

2018-12-21

How to Cite

REZENDE, R. K. S., SCOTON, A. M. N., JESUS, M. V., PEREIRA, Z. V., & PINTO, F. (2018). Callus Induction in Baru (Dipteryx alata Vog.) Explants. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47(2), 538–543. https://doi.org/10.15835/nbha47111366

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DOI: 10.15835/nbha47111366

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