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Transcriptome sequencing of two Kentucky bluegrass (Poa pratensis L.) genotypes in response to heat stress

Qiong LI, Yali HE, Mingyue TU, Junhui YAN, Liangliang YU, Weiwei QI, Xiaojun YUAN

Abstract


Kentucky bluegrass (Poa pratensis L.) (KBG) is a major cool-season turfgrass. However, as its complex genetic background and production modes, limited genomic and transcriptomic information of KBG was known so far. In this study, a transcriptome study between wild type material Ninglan (summer stress sensitive) and cultivar material KBG03 (summer stress tolerant) was conducted, under optimal (25 °C) and high (40 °C) temperatures. A total of 81.42 Gb clean reads were generated and de novo assembled into 110,784 unigenes with an average length of 1,105 bp. About 50% KBG unigenes were similar to the non-redundant (NR) database. Among the NR BLASTx top hits, 27.47% unigenes were matched to Brachypodium distachyon. After heat stress, a massive amount of unigenes showed significantly differential expression in both genotypes. After 2h heat stress, more specially up-regulated differentially expressed unigenes (DEGs) and less down-regulated DEGs were detected in Ninglan than in KBG03. At 24h, the expression of 1710 and 730 unigenes were increased and decreased uniquely in Ninglan, and 1361 up-regulated DEGs and 757 down-regulated DEGs were just found in KBG03. More heat shock proteins (HSPs) and heat shock transcription factors (HSFs) DEGs were also identified at 2h than 24h in both genotypes. In addition, by Gene Ontology (GO) enrichment analysis, three core terms (“protein folding”, “response to heat”, and “response to hydrogen peroxide”) of biological process (BP) ontology were found in both genotypes under different heat stress time. The DEGs shared in both genotypes might be related to the basic mechanism of thermal response in KBG.

 

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 1, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI number will become active after the article will be included in the complete issue.


Keywords


heat stress; transcriptome sequencing; turfgrass; unigenes

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References


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DOI: http://dx.doi.org/10.15835/nbha47111365

June 1, 2018: Notulae Botanicae Horti Agrobotanici Cluj-Napoca in Scopus – Elsevier CiteScore 2017=0.78, Horticulture; Agronomy and Crop Science; Plant Science


 
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