Identification of Nitric Oxide Responsive Genes in the Rudimentary Leaves of Litchi chinensis

Xingyu LU, Houbin CHEN, Zhiqun HU, Biyan ZHOU

Abstract


Litchi (Litchi chinensis Sonn.) is an evergreen woody fruit tree widely cultivated in subtropical and tropical regions. Warm winter and hot spring often leads to abnormal floral differentiation in litchi. Under this condition, the rudimentary leaves in the floral buds expand and the inflorescences will stop developing. Thus, how to promote abortion of rudimentary leaves in litchi inflorescence are important for floral development. Previous study indicated that nitric oxide (NO) produced by sodium nitroprusside (SNP) promoted flowering and abortion of rudimentary leaves in litchi. In the present study, a suppression subtractive hybridization (SSH) was used to identify NO responsive genes. As a result, 16 high homologous ESTs were obtained from the SSH library of the SNP treated rudimentary leaves. The ESTs were classified into three groups. They are disease/defensive, protein destination and storage, and protein synthesis. Quantitative reverse transcription PCR (qRT-PCR) analysis indicated that 6 out of the 7 randomly selected ESTs’expression showed an increasing trend from 0 h to 10 h of SNP treatment. It is suggested that the litchi homologs 18S ribosomal RNA gene, cytochrome P450 like TBP, and the senescence-associated protein, chaperone protein, and a hypothetical protein encoding genes may be involved in the NO-induced senescence in litchi rudimentary leaves. LcERD15-like may be a key gene involved in this process.


Keywords


abortion; nitric oxide; rudimentary leaves; suppression subtractive hybridization

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References


Alves MS, Fontes EPB, Fietto LG (2011a). Early responsive to dehydration 15, a new transcription factor that integrates stress signaling pathways. Plant Signaling and Behavior 6(12):1993-1996.

Alves MS, Reis PAB, Dadalto SP, Faria JAQA, Fontes EPB, Fietto LG (2011b). Anovel transcription factor, ERD15 (Early Responsive to Dehydration 15), connects endoplasmic reticulum stress with an osmotic stress-induced cell death signal. Journal of Biological Chemistry 286:20020-20030.

Beligni MV, Lamattina L (2001). Nitric oxide in plants: the history is just beginning. Plant, Cell and Environment 24(3):267-278.

Besson-Bard A, Astier J, Rasul S, Wawer I, Dubreuil-Maurizi C, Jeandroz S, Wendehenne D (2009). Current view of nitric oxide-responsive genes in plants. Plant Science 177(4):302-309.

Bevan M, Bancroft I, Bent E, Love K, Goodman H, Dean C, ... Chalwatzis N (1998). Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana. Nature 391(6666):485-488.

Chen HB, Huang HB (2005). Low temperature requirements for floral induction in lychee. Acta Horticulture 665:195-202.

Huang HB, Chen HB (2005). A phase approach towards floral formation in lychee. Acta Horticulturae 665:185-194.

Kariola T, Brader G, Helenius E, Li J, Heino P, Palva ET (2006). Early responsive to dehydration 15, a negative regulator of abscisic acid responses in Arabidopsis. Plant Physiology 142(4):1559-1573.

Kiyosue T, Yamaguchi-Shinozaki K, Shinozaki K (1994). Cloning of cDNAs for genes that are early-responsive to dehydration stress (ERDs) in Arabidopsis thaliana L.: identification of three ERDs as HSP cognate genes. Plant Molecular Biology 25(5):791-798.

Lindermayr C, Saalbach G, Durner J (2005). Proteomic identification of S-nitrosylated proteins in Arabidopsis. Plant Physiology 137(3):921-930.

Liu WW, Chen HB, Lu XY, Rahman MJ, Zhong S, Zhou BY (2015). Identification of nitric oxide responsive genes in the floral buds of Litchi chinensis. Biologia Plantarum 59(1): 115-122.

Liu WW, Kim HJ, Chen HB, Lu XY, Zhou BY (2013). Identification of MV-generated ROS responsive EST clones in floral buds of Litchi chinensis Sonn. Plant Cell Reports 32(9):1361-1372.

Livak KJ, Schmittgen TD (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2-DELTADELTACT method. Methods 25 (4):402-408.

Lu X, Kim H, Zhong S, Chen H, Hu Z, Zhou B (2014). De novo transcriptome assembly for rudimentary leaves in Litchi chinesis Sonn. and identification of differentially expressed genes in response to reactive oxygen species. BMC Genomics 15:805.

Menzel CM, Simpson DR (1988). Effect of temperature on growth and flowering of litchi (Litchi chinensis Sonn.) cultivars. Journal of Horticultural Science 63(2):349-360.

Palavan-Unsal N, Arisan D (2009). Nitric oxide signalling in plants. The Botanical Review 75(2):203-229.

Polverari A, Molesini B, Pezzotti M, Buonaurio R, Marte M, Delledonne M (2003). Nitric oxide-mediated transcriptional changes in Arabidopsis thaliana. Molecular Plant Microbe Interactions 16(12):1094-1105.

Wang Y, Lin A, Loake GJ, Chu C (2013a). H2O2-induced leaf cell death and the crosstalk of reactive nitric/oxygen species. Journal of Integrative Plant Biology 55(3):202-208.

Wang Y, Loake GJ, Chu C (2013b). Cross-talk of nitric oxide and reactive oxygen species in plant programed cell death. Frontiers in Plant Science 4:314.

Yang HF, Lu XY, Chen HB, Wang C-C, Zhou B-Y (2017). Low temperature-induced leaf senescence and the expression of senescence-related genes in the panicles of Litchi chinensis. Biologia Plantarum 61(2): 315-322.

Zhou B, Chen H, Huang X, Li N, Hu Z, Gao Z, Lu Y (2008). Rudimentary leaf abortion with the development of panicle in litchi: changes in ultrastructure, antioxidant enzymes and phytohormones. Scientia Horticulturae 117(3):288-296.

Zhou B, Li N, Zhang Z, Huang X, Chen H, Hu Z, Pang X, Liu W, Lu Y (2012). Hydrogen peroxide and nitric oxide promote reproductive growth in Litchi chinensis. Biologia Plantarum 56(2):321-329.

Zhou J, Pesacreta TC, Brown RC (1999). RNA isolation without gel formation from oligosaccharide-rich onion epidermis. Plant Molecular Biology Reporter 17(4):397-407.

Ziaf K, Hussain Munis MFH, Samin G, Zhang X, Li J, Zhang J, Ye Z (2016). Characterization of ERD15 gene from cultivated tomato (Solanum lycopersicum). Pakistan Journal of Agricultural Sciences 53(1):27-33.

Ziaf K, Loukehaich R, Gong P, Liu H, Han Q, Wang T, Li H, Ye Z (2011). A multiple stress-responsive gene ERD15 from Solanum pennellii confers stress tolerance in tobacco. Plant and Cell Physiology 52(6):1055-1067.




DOI: http://dx.doi.org/10.15835/nbha46211113

June 1, 2018: Notulae Botanicae Horti Agrobotanici Cluj-Napoca in Scopus – Elsevier CiteScore 2017=0.78, Horticulture; Agronomy and Crop Science; Plant Science


 
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