Antioxidant Activities of the Methanol Extracts of Various Parts of Phalaenopsis Orchids with White, Yellow, and Purple Flowers

Hoang Chinh NGUYEN, Kuan-Hung LIN, Meng-Yuan HUANG, Chi-Ming YANG, Tin-Han SHIH, Tung-Chuan HSIUNG, Yen-Chang LIN, Fun-Chi TSAO

Abstract


Phalaenopsis (Phal.) orchids including white, yellow, and purple flowers are some of the most important commercial orchids worldwide. These flowering plants can be considered to be promising sources of antioxidants since several medicinal orchids were shown to have potential antioxidant activities. The antioxidant activities and several secondary metabolite compounds of the methanolic extracts of four parts (the root, pedicel, leaf, and flower) of three hybrids of white (Phal. ‘City More’), yellow (Phal. ‘Sogo Meili’), and purple (Phal. ‘Queen Beer’) flowering orchids were investigated. Results showed that the highest levels of chlorophyll a and chlorophyll b were respectively obtained in leaf extracts of white and purple orchids, whereas carotenoid showed the highest content in the flower extract of the yellow orchid. Among all tested extracts, flavonoids and anthocyanin demonstrated the highest levels in the flower extract of the purple orchid, whereas the highest level of polyphenols was observed in the flower extract of the yellow orchid. The leaf extract of the white orchid was the most effective extract with a 50% inhibitory concentration in the DPPH-scavenging activity assay, while the highest ferrous iron-chelating effect was observed in flower extracts of the yellow orchid and purple orchid, and the pedicel extract of the purple orchid. In the reducing power assay, the flower extract of the white orchid showed the most potent extract, followed by the leaf extract of the yellow orchid and the flower extract of the purple orchid. Relationships between flower colors and antioxidant activities of these orchids showed them to be potential sources of antioxidants for both medicinal use and stress-tolerance in these orchids.


Full Text:

PDF

References


Beta T, Nam S, Dexter JE, Sapirstein HD (2017). Phenolic content and antioxidant activity of pearled wheat and roller-milled fractions. LWT-Food Science and Technology 78:151-159.

Bhattacharyya P, Kumaria S, Job N, Tandon P (2015a). Phyto-molecular profiling and assessment of antioxidant activity within micropropagated plants of Dendrobium thyrsiflorum: A threatened, medicinal orchid. Plant Cell Tissue and Organ Culture 122:535-550.

Bhattacharyya P, Kumaria S, Tandon P (2015b). Applicability of ISSR and DAMD markers for phyto-molecular characterization and association with some important biochemical traits of Dendrobium nobile, an endangered medicinal orchid. Phytochemistry 117:306-316.

Bhattacharyya P, Kumaria S, Tandon P (2016). High frequency regeneration protocol for Dendrobium nobile: A model tissue culture approach for propagation of medicinally important orchid species. South Africa Journal of Botany 104:232-243.

Brewer M (2011). Natural antioxidants: Sources, compounds, mechanisms of action, and potential applications. Comprehensive Reviews in Food Science and Food Safety 10:221-247.

Bulpitt C (2005). The uses and misuses of orchids in medicine. QJM: An International Journal of Medicine 98:625-631.

Caleja C, Barros L, Antonio AL, Oliveira MBP, Ferreira IC (2017). A comparative study between natural and synthetic antioxidants: Evaluation of their performance after incorporation into biscuits. Food Chemistry 216:342-346.

Chao PY, Lin SY, Lin KH, Liu YF, Hsu JI, Yang CM, Lai JY (2014). Antioxidant activity in extracts of 27 indigenous Taiwanese vegetables. Nutrients 6:2115-2130.

Chen C (2015). Application of growth models to evaluate the microenvironmental conditions using tissue culture plantlets of Phalaenopsis Sogo Yukidian ‘V3’. Scientia Horticulturae 191:25-30.

Chen CL, Tsai WH, Chen CJ, Pan TM (2016). Centella asiatica extract protects against amyloid ?1-40-induced neurotoxicity in neuronal cells by activating the antioxidative defence system. Journal of Traditional Complementary Medicine 6:362-369.

Chinsamy M, Finnie J, Van Staden J (2014). Anti-inflammatory, antioxidant, anti-cholinesterase activity and mutagenicity of South African medicinal orchids. South Africa Journal of Botany 91:88-98.

de la Rosa-Manzano E, Andrade JL, García-Mendoza E, Zotz G, Reyes-García C (2015). Photoprotection related to xanthophyll cycle pigments in epiphytic orchids acclimated at different light microenvironments in two tropical dry forests of the Yucatan Peninsula, Mexico. Planta 242:1425-1438.

Dinis TC, Madeira VM, Almeida LM (1994). Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Archives of Biochemistry and Biophysics 315:161-169.

Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocker P, Vidal N (2006). Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chemistry 97:654-660.

Giri L, Dhyani P, Rawat S, Bhatt ID, Nandi SK, Rawal RS, Pande V (2012). In vitro production of phenolic compounds and antioxidant activity in callus suspension cultures of Habenaria edgeworthii: A rare Himalayan medicinal orchid. Industrial Crops Production 39:1-6.

Guo WJ, Lin YZ, Lee N (2012). Photosynthetic light requirements and effects of low irradiance and daylength on Phalaenopsis amabilis. Journal of American Society of Horticulture Science 137:465-472.

He X, Fang J, Wang X, Zhao Z, Chang Y, Guo H, Huang L (2016). Bletilla striata: Medicinal uses, phytochemistry and pharmacological activities. Journal of Ethnopharmacology 195:20-38.

Hsing HX, Lin YJ, Tong CG, Li MJ, Chen YJ, Ko SS (2016). Efficient and heritable transformation of Phalaenopsis orchids. Botanical Study 57:30.

Huang MY, Lin KH, Lu CC, Chen LR, Hsiung TC, Chang WT (2017). The intensity of blue light-emitting diodes influences the antioxidant properties and sugar content of oyster mushrooms (Lentinus sajor-caju). Scientia Horticulturae 218:8-13.

Huang SS, Su SY, Chang JS, Lin HJ, Wu WT, Deng JS, Huang GJ (2016). Antioxidants, anti-inflammatory, and antidiabetic effects of the aqueous extracts from Glycine species and its bioactive compounds. Botanical Study 57:38.

Huang YC, Chang YH, Shao YY (2006). Effects of genotype and treatment on the antioxidant activity of sweet potato in Taiwan. Food Chemistry 98:529-538.

Hübner A, Neto A, Sobreira F, Pinto C, Dario M, Lourenço F, Baby A, Bacchi E (2016). Phytochemistry, antioxidant activity, and sunscreen efficacy of hydroethanolic extract of Cabernet Sauvignon grape pomace (Vitis vinifera L.). Planta Medicine 81:227.

Ledford H, Niyogi K (2005). Singlet oxygen and photo-oxidative stress management in plants and algae. Plant Cell Environment 28:1037-1045.

Mancinelli AL, Yang CPH, Lindquist P, Anderson O, Rabino I (1975). Photocontrol of anthocyanin synthesis III. The action of streptomycin on the synthesis of chlorophyll and anthocyanin. Plant Physiology 55:251-257.

Pant B (2013). Medicinal orchids and their uses: Tissue culture a potential alternative for conservation. Africa Journal of Plant Science 7:448-467.

Pokorný J (2007). Are natural antioxidants better–and safer–than synthetic antioxidants? European Journal of Lipid Science Technology 109:629-642.

Porra R, Thompson W, Kriedemann P (1989). Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: Verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochim Biophys Acta-Bioenergetics 975:384-394.

Prasad KN, Yang B, Shi J, Yu C, Zhao M, Xue S, Jiang Y (2010). Enhanced antioxidant and antityrosinase activities of longan fruit pericarp by ultra-high-pressure-assisted extraction. Journal of Pharmaceutical and Biomedical Analysis 51:471-477.

Prasad KN, Yang B, Yang S, Chen Y, Zhao M, Ashraf M, Jiang Y (2009). Identification of phenolic compounds and appraisal of antioxidant and antityrosinase activities from litchi (Litchi sinensis Sonn.) seeds. Food Chemistry 116:1-7.

Pulido R, Bravo L, Calixto FS (2000). Antioxidant activity of dietary polyphenolsas determined by a modified ferric reducing / antioxidant power assay. Journal of Agricultural Food Chemistry 48:3396-3402.

Rashmi K, Shweta S, Sudeshna C, Vrushala P, Kekuda TP, Raghavendra H (2015). Antibacterial and radical scavenging activity of selected orchids of Karnataka, India. Science, Technology and Arts Research Journal 4:160-164.

Rumbaoa RG, Cornago DF, Geronimo IM (2009). Phenolic content and antioxidant capacity of Philippine sweet potato (Ipomoea batatas) varieties. Food Chemistry 113:1133-1138.

Schuster R, Zeindl L, Holzer W, Khumpirapang N, Okonogi S, Viernstein H, Mueller M (2017). Eulophia macrobulbon – an orchid with significant anti-inflammatory and antioxidant effect and anticancerogenic potential exerted by its root extract. Phytomedicine 24:157-165.

Sikora E, Cieslik E, Leszczynska T, Filipiak-Florkiewicz A, Pisulewski PM (2008). The antioxidant activity of selected cruciferous vegetables subjected to aquathermal processing. Food Chemistry 107:55-59.

Stajner D, Popovic BM, Kapor A, Boza P, Stajner M (2010). Antioxidant and scavenging capacity of Anacamptis pyrimidalis L. – pyrimidal orchid from Vojvodina. Phytotherapy Research 24:759-763.

Sun A, Liu J, Pang S, Lin J, Xu R (2016). Two novel phenanthraquinones with anti-cancer activity isolated from Bletilla striata. Bioorganic & Medicinal Chemistry Letters 26:2375-2379.

Takahashi S, Badger M (2011). Photoprotection in plants: a new light on photosystem II damage. Trends in Plant Science 16:53-60.

Tatsuzawa F, Ichihara K, Shinoda K, Miyoshi K (2010). Flower colours and pigments in Disa hybrid (Orchidaceae). South African Journal of Botany 76:49-53.

Tsai CC, Chou CH, Wang HV, Ko YZ, Chiang TY, Chiang YC (2015). Biogeography of the Phalaenopsis amabilis species complex inferred from nuclear and plastid DNAs. BMC Plant Biology 15:202.

Yoshiki Y, Kahara T, Okubo K, Sakabe T, Yamasaki T (2001). Superoxide-and 1, 1-diphenyl-2-picrylhydrazyl radical-scavenging activities of soyasaponin ? g related to gallic acid. Bioscience, Biotechnology, and Biochemistry 65:2162-2165.

Zahara M, Datta A, Boonkorkaew P (2016). Effects of sucrose, carrot juice and culture media on growth and net CO2 exchange rate in Phalaenopsis hybrid ‘Pink’. Scientia Horticulturae 205:17-24.




DOI: http://dx.doi.org/10.15835/nbha46211038

June 1, 2017: Notulae Botanicae Horti Agrobotanici Cluj-Napoca in CiteScore rank (Scopus – Elsevier) 28/66 in Horticulture
 
http://not-bot-horti-agrobo.blogspot.com/
https://www.facebook.com/NotBotHA
https://twitter.com/NotBotHA