Physiological and Biochemical Responses of Common Bush Bean to Drought

Alefsi David SÁNCHEZ-REINOSO, Gustavo Adolfo LIGARRETO-MORENO, Hermann RESTREPO-DÍAZ

Abstract


Agriculture has been adversely affected by the low water availability resulting from climate change, creating environmental stress for the common bean (Phaseolus vulgaris L.). A growth room experiment was performed to evaluate the physiological and biochemical responses of the several bush bean genotypes to water deficit conditions. Plants in soil with 20 g∙L-1 polyethylene glycol 6000 (PEG) were subjected to drought for 15 d. The levels of photosynthesis, stomatal conductance and transpiration in all genotypes decreased by approximately 65% under water deficit conditions compared with the corresponding values in the controls. Water use efficiency was enhanced by water deficit conditions, with ʻBiancaʼ plants exhibiting the highest values (28.08 µmol∙mol-1), followed by ʻNUA35ʼ, ʻBachueʼ and ʻCerinzaʼ (20.46, 20.11 and 18.21 µmol∙mol-1, respectively). The ʻBiancaʼ plants exhibited a lower relative tolerance index (50%), and water deficit increased the levels of leaf photosynthetic pigments, chlorophyll and carotenoids in this genotype by approximately 100%. The photosynthetic efficiency, which was evaluated using the Fv/Fm ratio and rapid light-derived parameters (the maximum electron transport rate and a light saturation parameter), decreased due to water deficit conditions, particularly in the ʻBiancaʼ plants, in which these parameters were reduced by approximately 60%. The proline and malondialdehyde (MDA) contents were increased by the addition of PEG, primarily in the ʻBacatáʼ and ʻBiancaʼ plants. In conclusion, our results suggest that rapid light-response curves can be useful for characterizing genotypes because they represent an easy and non-destructive tool for understanding acclimatization mechanisms under water deficit conditions. In addition, all genotypes exhibited susceptibility to water deficit conditions, and the most susceptible genotype was ʻBiancaʼ, as reflected by a significant reduction in the electron transport rate and the presence of oxidative damage (high MDA content and electrolyte leakage), suggesting that this cultivar could not adapt well to landscaping situations in which periods of extreme water deficit can be expected.


Keywords


lipid peroxidation; photosynthesis; proline; rapid light-response curves; relative tolerance index

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References


Bates L, Waldren R, Teare I (1973). Rapid determination of free proline for water-stress studies. Plant and Soil 39:205-207.

Beebe S, Rao IM, Cajiao C, Grajales M (2008). Selection for drought resistance in common bean also improves yield in phosphorus limited and favorable environments. Crop Science 48:582-592.

Beebe S, Rao IM, Mukankusi C, Buruchara R (2012). Improving resource use efficiency and reducing risk of common bean production in Africa, Latin America and the Caribbean. In: Hershey C (Ed). Issues in tropical agriculture. I. Eco-efficiency: From vision to reality. International Center for Tropical Agriculture (CIAT), Cali, Colombia pp 117-134.

Beebe SE, Rao IM, Blair MW, Acosta-Gallegos JA (2013). Phenotyping common beans for adaptation to drought. Frontiers in Physiology 4:1-20.

Bradley RS, Vuille M, Diaz HF, Vergara W (2006). Threats to water supplies in the tropical Andes. Science 312:1755-1756.

Chaves MM, Pereira JS, Maroco J, Rodrigues ML, Ricardo CPP, Osorio ML, Carvalho I, Faria T, Pinheiro C (2002). How plants cope with water stress in the field? Photosynthesis and growth. Annals of Botany 89:907-916.

Clavijo-Sánchez N, Flórez-Velasco N, Restrepo-Díaz H (2015). Potassium Nutritional Status Affects Physiological Response of Tamarillo Plants (Cyphomandra betacea Cav.) to Drought Stress. Journal of Agricultural Science and Technology 17:1839-1849.

Costa-Franca MG, Pham-Thi AT, Pimentel C, Pereyra-Rossiello RO, Zuily-Fodil Y, Laffray D (2000). Differences in growth and water relations among Phaseolus vulgaris cultivars in response to induced drought stress. Environmental and Experimental Botany 43:227-237.

Dai A (2011). Drought under global warming: A review. WIREs Climate Change 2:45-65.

Darkwa K, Ambachewa D, Mohammed H, Asfawa A, Blair MW (2016). Evaluation of common bean (Phaseolus vulgaris L.) genotypes for drought stress adaptation in Ethiopia. The Crop Journal 4:367-376.

De Laat D, Colombo C, Chiorato A, Carbonell S (2014). Induction of ferritin synthesis by water deficit and iron excess in common bean (Phaseolus vulgaris L.). Molecular Biology Reports 41:1427-1435.

Dutta Gupta S, Auge RM, Denchev PD, Conger BV (1995). Growth, proline accumulation and water relations in NaCl-selected and non-selected callus lines of Dactylis glomerata. Environmental and Experimental Botany 35:83-92.

Farooq M, Wahid A, Kobayashi N, Fujita D, Basra SMA (2009). Plant drought stress: Effects, mechanisms and management. Agronomy for Sustainable Development 29:185-212.

Fernandez CGJ (1993). Effective selection criteria for assessing plant stress tolerance. In: Kuo CG (Ed). Adaptation of food crops to temperature and water stress: Proceedings of an international symposium. Asian Vegetable Research and Development Centre, Shanhua, Taiwan pp 257-270.

George S, Minhas NM, Jatoi SA, Siddiqui SU, Ghafoor A (2015). Impact of polyethylene glycol on proline and membrane stability index for water stress regime in tomato (Solanum lycopersicum). Pakistan Journal of Botany 47:835-844.

Ghanbari AA, Mousavi SH, Mousapour-Gorji A, Rao I (2013). Effects of water stress on leaves and Seeds of bean (Phaseolus vulgaris L.). Turkish Journal of Field Crops 18:73-77.

Hodges DM, DeLong JM, Forney CF, Prange RK (1999). Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta 207:604-611.

Keyvan S (2010). The effects of drought stress on yield, relative water content, proline, soluble carbohydrates and chlorophyll of bread wheat cultivars. Journal of Animal and Plant Science 8:1051-1060.

Kiani-Pouya A, Rasouli F (2014). The potential of leaf chlorophyll content to screen bread-wheat genotypes in saline condition. Photosyntetica 52:288-300.

Lanna AC, Mitsuzono ST, Terra TGR, Vianello RP, Carvalho MAF (2016). Physiological characterization of common bean (Phaseolus vulgaris L.) genotypes, water-stress induced with contrasting response towards drought. Australian Journal of Crop Science 10:1-6.

Liu, H., Sultan MARF, Zhao HX (2013). The screening of water stress tolerant wheat cultivars with physiological indices. Global Journal Of Biodiversity Science And Management 3:211-218.

Lobell DB, Gourdji SM (2012). The influence of climate change on global crop productivity. Plant Physiology 160:1686-1697.

Lu C, Zhang J (1999). Effects of water stress on photosystem II photochemistry and its thermostability in wheat plants. Journal of Experimental Botany 50:1199-1206.

Maxwell, K, Jhonson GN (2000). Chlorophyll fluorescence-a practical guide. Journal of Experimental Botany 51:659-668.

Mathobo R, Maraisa D, Steyn JM (2017). The effect of drought stress on yield, leaf gaseous exchange andchlorophyll fluorescence of dry beans (Phaseolus vulgaris L.). Agricultural Water Management 180:118-125.

Munoz-Perea CG, Allen RG, Westermann DT, Wright JL, Singh SP (2007). Water use efficiency among dry bean landraces and cultivars in drought-stressed and non-stressed environments. Euphytica 155:393-402.

Naser L, Kourosh V, Bahman K, Reza A (2010). Soluble sugars and proline accumulation play a role as effective indices for drought tolerance screening in Persian walnut (Juglans regia L.) during germination. Fruits 65:97-112.

Omae H, Kumar A, Shono M (2012). Adaptation to high temperature and water deficit in the common bean (Phaseolus vulgaris L.) during the reproductive period. Journal of Botany 2012:1-6.

Polanía JA, Rao IM, Beebe S, García R (2009). Root development and distribution under drought stress in common bean (Phaseolus vulgaris L.) in a soil tube system. Agronomía Colombiana 27:25-32.

Polania J, Rao IM, Cajiao C, Rivera M, Raatz B, Beebe S (2016). Physiological traits associated with drought resistance in Andean and Mesoamerican genotypes of common bean (Phaseolus vulgaris L.). Euphytica 210:17-29.

Ralph PJ, Polk SM, Moore KA, Orth RJ, Smith, Jr. WO (2002). Operation of the xanthophyll cycle in the seagrass (Zostera marina) in response to variable irradiance. Journal of Experimental Marine Biology and Ecology 271:189-207.

Ramírez-Villegas J, Salazar M, Jarvis A, Navarro-Racines CE (2012). A way forward on adaptation to climate change in Colombian agriculture: perspectives towards 2050. Climatic Change 115:611-628.

Restrepo-Díaz H, Melgar JC, Lombardini L (2010). Ecophysiology of horticultural crops: An overview. Agronomía Colombiana 28:71-79.

Rosales MA, Ocampo E, Rodríguez-Valentín R, Olvera-Carrillo Y, Acosta-Gallegos J, Covarrubias AA (2012). Physiological analysis of common bean (Phaseolus vulgaris L.) cultivars uncovers characteristics related to terminal drought resistance. Plant Physiology and Biochemistry 56:24-34.

Sánchez-Reinoso AD, Garcés-Varón G, Restrepo-Díaz H (2014). Biochemical and physiological characterization of three rice cultivars under different daytime temperature conditions. Chilean Journal of Agricultural Research 74:373-379.

Sánchez-Rodríguez E, Rubio-Wilhelmi M, Cervilla LM, Blasco JJ, Rios JJ, Rosales MA, Romero L, Ruiz JM (2010). Genotypic differences in some physiological parameters symptomatic for oxidative stress under moderate drought in tomato plants. Plant Science 178:30-40.

Sayed OH (2003). Chlorophyll fluorescence as a tool in cereal crop research. Photosynthetica 41:321-330.

Siddiqui MH, Al-Khaishany MY, Al-Qutami MA, Al-Whaibi MH, Grover A, Ali HM, Al-Wahibi MS, Bukhari NA (2015). response of different genotypes of Faba bean plant to drought stress. International Journal of Molecular Sciences 16:10214-10227.

Silva MA, Jifon JL, Da Silva JAG, Sharma V (2007). Use of physiological parameters as fast tools to screen for drought tolerance in sugarcane. Brazilian Journal of Plant Physiology 19:193-201.

Song R, Zhao CY, Liu J, Zhang J, Du YX, Li JZ, Sun HZ, Zhao HB, Zhao QZ (2013). Effect of sulphate nutrition on arsenic translocation and photosynthesis of rice seedlings. Acta Physiologia Plantarum 35:3237-3243.

Wellburn AR (1994). The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. Journal of Plant Physiology 144:307-313.

White JW, Castillo JA (1992). Evaluation of diverse shoot genotypes on selected root genotypes of common bean under soil water deficits. Crop Science 32:762-765.

Xu WZ, Deng XP, Xu BC, Gao ZJ, Ding WL (2014). Photosynthetic activity and efficiency of Bothriochloa ischaemum and Lespedeza davurica in mixtures across growth periods under water stress. Acta Physiologia Plantarum 36:1033-1044.




DOI: http://dx.doi.org/10.15835/nbha46210965

June 1, 2018: Notulae Botanicae Horti Agrobotanici Cluj-Napoca in Scopus – Elsevier CiteScore 2017=0.78, Horticulture; Agronomy and Crop Science; Plant Science


 
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